Abstract：
:The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two major open reading frames encoded on the same strand but in different frames. The smaller, ORF1 (435 bp), is preceded by Shine Dalgarno (SD) and Escherichia coli-10, -35 sequences and is predicted to encode an acidic polypeptide of 145 aa. The putative protein shows little homology to known bacterial proteins except in a small region similar to part of the Pre protein (plasmid recombination enzyme) of Bacillus plasmid pTB913. Pre proteins of the pTB913 family, however, show conservation of several amino acids in the amino terminal region of the protein that may be related to function. This pattern of amino acid conservation is not observed in ORF1. The predicted product of ORF2 (849 bp) is a basic protein of 283 as that shows significant similarity to repA gene products of plasmids from gram-negative (but not gram-positive) bacteria. No typical Shine Dalgarno sequence or -10, -35 sequences precede this coding region. While sequence homologous to nick sites of plasmids from gram-positive organisms that replicate via ssDNA intermediates were also detected in pRJF1, the order of the three consecutive inverted repeats typical of nick sites is not conserved. pRJF1, however, does contain an extended region of directly and inversely repeated sequence motifs reminiscent of theta replication origins.
journal_name
Plasmidjournal_title
Plasmidauthors
Hefford MA,Teather RM,Forster RJdoi
10.1006/plas.1993.1008subject
Has Abstractpub_date
1993-01-01 00:00:00pages
63-9issue
1eissn
0147-619Xissn
1095-9890pii
S0147-619X(83)71008-5journal_volume
29pub_type
杂志文章相关文献
PLASMID文献大全abstract：:The stable maintenance of certain plasmids in bacterial populations has contributed significantly to the current worldwide antibiotic resistance (AbR) emergency. IncX plasmids, long underestimated in this regard, have achieved recent notoriety for their roles in transmission of resistance to carbapenem and colistin, t...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2017.02.006
更新日期：2017-05-01 00:00:00
abstract：:The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1999.1399
更新日期：1999-05-01 00:00:00
abstract：:Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2018.01.002
更新日期：2018-01-01 00:00:00
abstract：:We present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pKLH2 from an environmental bacterium, Acinetobacter calcoaceticus, isolated from a mercury mine. The pKLH2 mer operon has essentially the same gene organization as that of Tn21 and Tn501 from clinical bacteria. The pKLH...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1993.1064
更新日期：1993-11-01 00:00:00
abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2019.01.002
更新日期：2019-03-01 00:00:00
abstract：:As part of a study identifying plasmids in Campylobacter, we isolated and sequenced two novel cryptic plasmids from an agricultural isolate of Campylobacter coli. The larger of the two plasmids, p3384, is 3316 bp in length and has a G+C content of 31.18%. A typical origin of replication consisting of five iterons was ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2005.06.001
更新日期：2006-01-01 00:00:00
abstract：:The plasmid pMSP3535 is a popular vector for nisin-inducible expression of heterologous genes in lactic acid bacteria. However, the maximum protein expression level achievable with pMSP3535 is relatively low. In an effort to increase expression we modified pMSP3535 to create a high expression variant termed pMSP3535H2...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2007.05.004
更新日期：2007-11-01 00:00:00
abstract：:Glycopeptide resistant Enterococcus faecium (GREF) persists on Norwegian poultry farms despite the ban on the growth promoter avoparcin. The biological basis for long-term persistence of avoparcin resistance is not fully understood. This study presents the complete DNA sequence of the E. faecium R-plasmid pVEF3 and fu...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2008.04.002
更新日期：2008-07-01 00:00:00
abstract：:We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb E...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1063
更新日期：1994-11-01 00:00:00
abstract：:Almost 200 scientists from America, Europe, Asia, Australia, and Africa participated in the Plasmid Biology 2004 meeting, which was organized between 15th and 21st September 2004 in Kanoni (Corfu island), Greece. Various aspects of biology of plasmids and other mobile genetic elements were discussed during the meeting...
journal_title：Plasmid
pub_type：
doi：10.1016/j.plasmid.2004.10.002
更新日期：2005-01-01 00:00:00
abstract：:Mutations that increase the copy number of the pSC101 replicon have been used for construction of new cloning vectors. Replacement of glutamate at position 93 in RepA yields plasmids that replicate at medium (27 copies/cell) and high (approximately 240 copies/cell) copy numbers. Based on the crystal structure of RepE,...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2008.01.004
更新日期：2008-05-01 00:00:00
abstract：:From Enterococcus faecalis cells containing random chromosomal insertions of Tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. The phage-resistant strains all showed decreased recipient ability (Con-) in broth matings with donors carrying pheromone-inducible plasmids. Thes...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(90)90025-8
更新日期：1990-07-01 00:00:00
abstract：:A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identit...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2006.05.007
更新日期：2006-11-01 00:00:00
abstract：:Recently an insertional mutagenesis procedure has been developed to permit cloning of genes affected in developmental mutants of Dictyostelium discoideum (Kuspa and Loomis, Proc. Natl. Acad. Sci. USA 89, 8803-8807, 1992). In this procedure a plasmid bearing the URA (pyr5-6) gene is linearized with a restriction enzyme...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1995.0003
更新日期：1995-11-01 00:00:00
abstract：:A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/ Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1996.0011
更新日期：1996-03-01 00:00:00
abstract：:Bacillus mycoides, a member of the Bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. Two plasmids, one from a strain curving to the right (pDx14.2),...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2005.05.002
更新日期：2005-11-01 00:00:00
abstract：:We have constructed shuttle vectors for integration of genes via double homologous recombination into three ectopic sites on the chromosome of Bacillus subtilis. The sites of integration are the pyrD, gltA, and sacA genes located at 139 degrees, 172 degrees, and 333 degrees, respectively, on the chromosome. Integratio...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2004.01.006
更新日期：2004-05-01 00:00:00
abstract：:A novel type I restriction-modification specificity subunit, S. LlaW12I, has been identified on the naturally occurring 8.0-kb plasmid pAW122 in the lactic acid bacterium Lactococcus lactis subsp. cremoris W12. Presence of the HsdS protein together with a complete type I restriction-modification system conferred incre...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.2000.1478
更新日期：2000-09-01 00:00:00
abstract：:Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a pu...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1995.9995
更新日期：1995-09-01 00:00:00
abstract：:Recently the plasmid RK2 replication initiation protein, TrfA, has been shown to bind to the beta subunit of DNA Polymerase III (DnaN) via a short pentapeptide with the consensus QL[S/D]LF. A second consensus peptide, the hexapeptide QLxLxL, has also been demonstrated to mediate binding to DnaN. Here we describe the r...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2006.07.005
更新日期：2007-01-01 00:00:00
abstract：:Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2014.05.004
更新日期：2014-07-01 00:00:00
abstract：:Mobile plasmid-encoded elements are DNA segments that are transferred for horizontal gene transfer and that confer adaptive proprieties, as well as virulence and antibiotic and heavy metal resistance to bacteria. The conjugative plasmid pUM505, isolated from a clinical strain of Pseudomonas aeruginosa, possesses a put...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2018.07.003
更新日期：2018-06-01 00:00:00
abstract：:The chimeric plasmid pBN183 was first constructed in Escherichia coli by ligating the BamHI-digested E. coli plasmid pBR322 and a Bg/II-linearized streptococcal plasmid, pNZ18. The pBN183 transformed E. coli to ApR at a frequency of (8.2 +/- 1.2) x 10(5) colony forming units (CFU)/microgram DNA. Electrotransformation ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(92)90033-7
更新日期：1992-07-01 00:00:00
abstract：:The gene(s) for chromosome-mediated beta-lactamase production of Proteus vulgaris GN7919 was cloned into a unique EcoRI site of pACYC184 as an insert of a 14.2-kb fragment, which was further digested into two fragments with EcoRI, 4.9 and 9.3 kb. The restriction enzyme digestion pattern of the recombinant plasmid, des...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90039-4
更新日期：1987-09-01 00:00:00
abstract：:Escherichia coli pcnB gene was discovered as a regulator of ColE1 plasmid copy number about 20 years ago. Further studies indicated that this gene codes for poly(A) polymerase I (PAP I). However, although importance of RNA polyadenylation has been demonstrated and biochemical properties of PAP I have been studied exte...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2005.10.002
更新日期：2006-03-01 00:00:00
abstract：:The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(89)90019-x
更新日期：1989-09-01 00:00:00
abstract：:Transcription of the 7.5-kb cryptic plasmid of Chlamydia trachomatis serovar L2 was investigated. Faint, diffuse transcripts of about 1.6 and 2.2 kb and intense short transcripts of about 250 and 430 bases were identified by Northern blot analysis. The short transcripts were found to have a common 5' end corresponding...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(92)90056-g
更新日期：1992-11-01 00:00:00
abstract：:Bacterial plasmids and phages encode the synthesis of toxic molecules that inhibit protozoan predation. One such toxic molecule is violacein, a purple pigmented, anti-tumour antibiotic produced by the Gram-negative soil bacterium Chromobacterium violaceum. In the current experiments a range of Escherichia coli K12 str...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2011.07.006
更新日期：2011-09-01 00:00:00
abstract：:We isolated a small multicopy cryptic plasmid, pNHK101, from Thermus sp. TK10 for use as a replicon of a Thermus expression vector. The nucleotide sequence of pNHK101 revealed that this plasmid was 1564bp long, with a total G+C content of 66.8%, which was in agreement with that of Thermus genomic DNA. The sequence did...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2004.12.004
更新日期：2005-07-01 00:00:00
abstract：:Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.2002.1565
更新日期：2002-03-01 00:00:00
