Cloning and expression of the gene(s) for chromosome-mediated beta-lactamase production of Proteus vulgaris in Escherichia coli.

abstract：:The 4.2-kb cryptic plasmid pVA380-1 has been used as a vector for the cloning of antibiotic resistance genes directly in streptococci, and in the construction of Escherichia coli/Streptococcus shuttle vectors. The results of subcloning experiments located the basic replicon of pVA380-1 within a 2.5-kb region. The nucl...

journal_title：Plasmid

authors： LeBlanc DJ,Lee LN,Abu-Al-Jaibat A

更新日期：1992-09-01 00:00:00

abstract：:Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging fro...

journal_title：Plasmid

authors： Fornari CS,Watkins M,Kaplan S

更新日期：1984-01-01 00:00:00

abstract：:The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...

journal_title：Plasmid

authors： Hefford MA,Teather RM,Forster RJ

更新日期：1993-01-01 00:00:00

abstract：:Most Acinetobacter plasmids are genus specific but their properties have not been investigated. Small plasmids with Rep_3 family replication initiation proteins and iterons are common in Acinetobacter baumannii and often carry antibiotic resistance genes and toxin-antitoxin systems. A RepAci1 plasmid, carrying the car...

journal_title：Plasmid

authors： Blackwell GA,Hall RM

更新日期：2019-05-01 00:00:00

abstract：:DNA replication initiation has been well-characterized; however, studies in the past few years have shown that there are still important discoveries to be made. Recent publications concerning the bacterial DnaA protein have revealed how this replication initiator, via interaction with specific sequences within the ori...

journal_title：Plasmid

authors： Zabrocka E,Wegrzyn K,Konieczny I

更新日期：2014-11-01 00:00:00

abstract：:Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....

journal_title：Plasmid

authors： Dumans AT,Carvalho MF,Coelho A

更新日期：1989-11-01 00:00:00

abstract：:Plasmid pT181 is a small multicopy plasmid from Staphylococcus aureus that belongs to incompatibility group 3 and expresses two distinct types of incompatibility, Inc3A and Inc3B. Inc3A incompatibility is expressed by the primary replication control determinant, copA, which specifies two small transcripts, RNA I and R...

journal_title：Plasmid

authors： Highlander SK,Novick RP

更新日期：1990-01-01 00:00:00

abstract：:The replication (rep) regions of small plasmids from three Sinorhizobium meliloti strains were cloned by marker rescue. Two unique replication regions were identified, one of which was common to two different strains. Plasmid pBB83 carried a 7.2 kbp rep region from a 42 kbp plasmid, and pBB84 carried a 4.5 kbp rep reg...

journal_title：Plasmid

authors： Watson RJ,Heys R

更新日期：2006-03-01 00:00:00

abstract：:The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...

journal_title：Plasmid

authors： Guay GG,Khan SA,Rothstein DM

更新日期：1993-09-01 00:00:00

abstract：:The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into...

journal_title：Plasmid

authors： Jobling MG,Peters SE,Ritchie DA

更新日期：1988-09-01 00:00:00

abstract：:Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...

journal_title：Plasmid

authors： Strunnikov AV

更新日期：2006-03-01 00:00:00

abstract：:We report the characterization of Tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the R-plasmid pGD10. Tn602 is highly homologous to the previously characterized Tn903, present on the R-plasmid R6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-I (hom...

journal_title：Plasmid

authors： Stibitz S,Davies JE

更新日期：1987-05-01 00:00:00

abstract：:Nonviral gene delivery has gained a lot of interest as a promising approach for gene therapy. Despite intensive studies and much progress the outcome of nonviral vectors has remained significantly weaker than that of viral vectors. A weak transfection efficiency of nonviral gene transfection is still limiting their in...

journal_title：Plasmid

authors： Soininen P,Hanzlíková M,Paukkunen M,Lecklin A,Männistö PT,Raasmaja A

更新日期：2010-01-01 00:00:00

abstract：:A new insertion variant of IS231 family, designated ISBth4, was identified from Bacillus thuringiensis MEX312. ISBth4 is 2046bp in length and is delimited by two 17bp inverted repeats (IR) with one mismatch, flanked by two perfect 11bp direct repeats (DR). ISBth4 contains two open reading frames (ORFs), ORF1 and ORF2,...

journal_title：Plasmid

authors： Wang J,Chaudhuri RR,Ellar DJ

更新日期：2010-01-01 00:00:00

abstract：:We report the construction of two filamentous fungi Trichoderma reesei expression vectors, pWEF31 and pWEF32. Both vectors possess the hygromycin phosphotransferase B gene expression cassette and the strong promoter and terminator of the cellobiohydrolase 1 gene (cbh1) from T. reesei. The two newly constructed vectors...

journal_title：Plasmid

authors： Lv D,Wang W,Wei D

更新日期：2012-01-01 00:00:00

abstract：:Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...

journal_title：Plasmid

authors： Peijnenburg AC,Breed PV,Bron S,Venema G

更新日期：1989-05-01 00:00:00

abstract：:Four plasmids encoding restriction and modification (R/M) systems are described that are different in the specificity of their restrictive activity toward the small isometric phage p2 and prolate phage c2. The R/M plasmids were cotransformed into Lactococcus lactis MG1363 with pVS2, encoding resistance to chlorampheni...

journal_title：Plasmid

authors： Josephsen J,Klaenhammer T

更新日期：1990-01-01 00:00:00

abstract：:The plasmid pMSP3535 is a popular vector for nisin-inducible expression of heterologous genes in lactic acid bacteria. However, the maximum protein expression level achievable with pMSP3535 is relatively low. In an effort to increase expression we modified pMSP3535 to create a high expression variant termed pMSP3535H2...

journal_title：Plasmid

authors： Kim JH,Mills DA

更新日期：2007-11-01 00:00:00

abstract：:Escherichia coli pcnB gene was discovered as a regulator of ColE1 plasmid copy number about 20 years ago. Further studies indicated that this gene codes for poly(A) polymerase I (PAP I). However, although importance of RNA polyadenylation has been demonstrated and biochemical properties of PAP I have been studied exte...

journal_title：Plasmid

authors： Jasiecki J,Wegrzyn G

更新日期：2006-03-01 00:00:00

abstract：:A new cryptic plasmid from a multi-resistant, multi-plasmid clinical strain of Escherichia coli has been isolated. The sequence of the 4072-base-pair pIGWZ12 (GenBank Accession No. DQ311641) was determined and analyzed. Two open-reading frames that code for proteins involved in plasmid mobilization and initiation of r...

journal_title：Plasmid

authors： Zaleski P,Wolinowska R,Strzezek K,Lakomy A,Plucienniczak A

更新日期：2006-11-01 00:00:00

abstract：:Two independently isolated temperature-sensitive bacteriophage that are specific for enterobacterial hosts harboring HI and HII plasmids were characterized to determine if any identifiable differences existed between them. The traits examined included adsorption pattern of phage to H pili, bacteriophage size, sensitiv...

journal_title：Plasmid

authors： Maher D,Sherburne R,Taylor DE

更新日期：1991-09-01 00:00:00

abstract：:The construction of a set of new plasmids that are suitable as general cloning vectors in Escherichia coli and Agrobacterium tumefaciens is described. Plasmid pUCD2 is amplifiable in E. coli, replicates in a wide range of gram-negative hosts and contains a number of useful restriction endonuclear cleavage sites and an...

journal_title：Plasmid

authors： Close TJ,Zaitlin D,Kado CI

更新日期：1984-09-01 00:00:00

abstract：:This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...

journal_title：Plasmid

authors： Khan SA

更新日期：2005-03-01 00:00:00

abstract：:The effects of the single-strand origins (SSOs) of plasmid pMV158 on (i) the conversion of its single-stranded (ss) replication intermediates to double-stranded (ds) plasmid DNA and (ii) its maintenance were analyzed. The rolling-circle plasmid pMV158, which replicates via ssDNA intermediates, contains two single-stra...

journal_title：Plasmid

authors： Meijer WJ,van der Lelie D,Venema G,Bron S

更新日期：1995-03-01 00:00:00

abstract：:Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence ...

journal_title：Plasmid

authors： Tabata A,Deutsch D,Otsuka S,Verratti K,Tomoyasu T,Nagamune H,Fischetti VA

更新日期：2018-01-01 00:00:00

abstract：:Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...

journal_title：Plasmid

authors： Manganelli R,Romano L,Ricci S,Zazzi M,Pozzi G

更新日期：1995-07-01 00:00:00

abstract：:Despite the prevalence of Arthrobacter in the environment little is known about their plasmids, or the capacity of Arthrobacter plasmids to mediate horizontal gene transfer. In this study, we compared eight plasmids from five Arthrobacter strains in order to identify putative core maintenance genes for replication, se...

journal_title：Plasmid

authors： Jerke K,Nakatsu CH,Beasley F,Konopka A

更新日期：2008-03-01 00:00:00

abstract：:A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-d...

journal_title：Plasmid

authors： Projan SJ,Carleton S,Novick RP

更新日期：1983-03-01 00:00:00

abstract：:The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...

journal_title：Plasmid

authors： Bartosik D,Baj J,Piechucka E,Waker E,Wlodarczyk M

更新日期：2002-09-01 00:00:00

abstract：:Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...

journal_title：Plasmid

authors： Jo C,Jo SA

更新日期：2001-01-01 00:00:00