Abstract:
:Four plasmids encoding restriction and modification (R/M) systems are described that are different in the specificity of their restrictive activity toward the small isometric phage p2 and prolate phage c2. The R/M plasmids were cotransformed into Lactococcus lactis MG1363 with pVS2, encoding resistance to chloramphenicol and erythromycin, to indicate successful transformation events. Analysis of cotransformants showed that three different R/M plasmids could be combined in L. lactis MG1363. The efficiency at which phage plaqued on the transformants decreased as the number of R/M plasmids increased. Some plasmid combinations were unstable suggesting replicon incompatibility.
journal_name
Plasmidjournal_title
Plasmidauthors
Josephsen J,Klaenhammer Tdoi
10.1016/0147-619x(90)90046-fsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
71-5issue
1eissn
0147-619Xissn
1095-9890pii
0147-619X(90)90046-Fjournal_volume
23pub_type
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