Abstract:
:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and partial digests. Analysis of a deletion mutant derived by in vitro digestion with PstI and of transfer-defective and tetracycline-sensitive deletion mutants of R27 derived by Tn5 insertion were instrumental in determining the positions of some fragments.
journal_name
Plasmidjournal_title
Plasmidauthors
Taylor DE,Brose ECdoi
10.1016/0147-619x(85)90058-7subject
Has Abstractpub_date
1985-01-01 00:00:00pages
75-7issue
1eissn
0147-619Xissn
1095-9890pii
0147-619X(85)90058-7journal_volume
13pub_type
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