pMH11, A tool for gene disruption and expression analysis in Azorhizobium caulinodans.

abstract：:pBTK445 is a newly described large (∼60Kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph Advenella kashmirensis. Based on its minimal replication region, a shuttle vector, pBTKS was constructed which can be used for diverse Alcaligenaceae members. The construct was found to be stab...

journal_title：Plasmid

authors： Dam B

更新日期：2011-03-01 00:00:00

abstract：:A new class of binary vectors has been constructed, containing the origin of replication of the W-type plasmid Sa. These vectors are designed to express foreign genes in plants under control of the TR gene 2' promoter or the promoter of a light-inducible ribulose-1,5-bisphosphate carboxylase small subunit gene of Arab...

journal_title：Plasmid

authors： Alliotte T,Zhu LH,Van Montagu M,Inzé D

更新日期：1988-05-01 00:00:00

abstract：:Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjug...

journal_title：Plasmid

authors： Dunn AK,Martin MO,Stabb EV

更新日期：2005-09-01 00:00:00

abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

journal_title：Plasmid

authors： Chiang CS,Bremer H

更新日期：1991-11-01 00:00:00

abstract：:The Tra1 region of RP1 from a derivative with Tn7 inserted into the kanamycin resistance determinant was cloned, using EcoRI, into the multicopy vector plasmid pBR325. For one orientation of the cloned fragment the resultant chimeric plasmid was very frequently lost from the cell, but in the other orientation it was m...

journal_title：Plasmid

authors： Watson J,Schmidt L,Willetts N

更新日期：1980-09-01 00:00:00

abstract：:Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...

journal_title：Plasmid

authors： Jo C,Jo SA

更新日期：2001-01-01 00:00:00

abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....

journal_title：Plasmid

authors： Moran RA,Anantham S,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:The effects of mutations have been studied within the apocytochrome b gene on the processing of transcripts from the gene for subunit 1 of cytochrome c oxidase (coxI). Most mutations which affect the expression of the reading frame encoded by the fourth intron of the apocytochrome b gene (bI4) result in a failure to r...

journal_title：Plasmid

authors： Hensgens LA,Van der Horst G,Grivell LA

更新日期：1984-07-01 00:00:00

abstract：:The 4.2-kb cryptic plasmid pVA380-1 has been used as a vector for the cloning of antibiotic resistance genes directly in streptococci, and in the construction of Escherichia coli/Streptococcus shuttle vectors. The results of subcloning experiments located the basic replicon of pVA380-1 within a 2.5-kb region. The nucl...

journal_title：Plasmid

authors： LeBlanc DJ,Lee LN,Abu-Al-Jaibat A

更新日期：1992-09-01 00:00:00

abstract：:Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...

journal_title：Plasmid

authors： Bahk JD,Kioka N,Sakai H,Komano T

更新日期：1988-11-01 00:00:00

abstract：:A suppressor of the mutations which inhibit the establishment of lysogeny in Pseudomonas aeruginosa has been found to be encoded by P. aeruginosa plasmids of four separate incompatibility groups. The wide distribution of this phenotype (designated Sly for suppressor of lysogeny establishment deficiency) indicates that...

journal_title：Plasmid

authors： Mondello FJ,Miller RV

更新日期：1984-03-01 00:00:00

abstract：:A chimeric plasmid, pRS7Rep (6.1 kb), was constructed using the replication region of pRS7, a large plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli using its pIJ2925 componen...

journal_title：Plasmid

authors： Rodríguez MC,Alegre MT,Martín MC,Mesas JM

更新日期：2015-01-01 00:00:00

abstract：:A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...

journal_title：Plasmid

authors： Menéndez C,Igloi GL,Brandsch R

更新日期：1997-01-01 00:00:00

abstract：:Two B/O plasmids were sequenced. The kanamycin resistance plasmid R805a was found in a Salmonella Typhi strain from a 1972 typhoid fever outbreak in Mexico City. pCERC6, which confers resistance to ampicillin, streptomycin and sulphamethoxazole, was found in a commensal E. coli isolated from a healthy adult in Sydney ...

journal_title：Plasmid

authors： Moran RA,Richardson IA,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...

journal_title：Plasmid

authors： Caufield PW,Shah G,Hollingshead SK,Parrot M,Lavoie MC

更新日期：1990-09-01 00:00:00

abstract：:Sphingomonas sp. A1 (strain A1) is capable of directly incorporating macromolecules (e.g., alginate) through the specialized import system--"super-channel." Here, we report the complete DNA sequence and genetic organization of plasmid pA1 from strain A1. Nucleotide sequence analysis revealed that pA1 comprises 46,557 ...

journal_title：Plasmid

authors： Harada KM,Aso Y,Hashimoto W,Mikami B,Murata K

更新日期：2006-07-01 00:00:00

abstract：:By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sens...

journal_title：Plasmid

authors： Maas R

更新日期：1983-11-01 00:00:00

abstract：:Transformation of Escherichia coli with Dictyostelium discoideum genomic DNA containing integrated shuttle vectors in multicopy, tandemly duplicated format resulted in the establishment of the linear plasmid molecules as circular monomeric replicons. The transformation efficiencies were comparable to those obtained wi...

journal_title：Plasmid

authors： Barth C,Wilczynska Z,Pontes L,Fraser DJ,Fisher PR

更新日期：1996-09-01 00:00:00

abstract：:We report here the molecular characterization of pFNL10, a 3990-bp cryptic plasmid of Francisella novicida-like F6168. The plasmid was maintained in F. novicida Utah 112 and F. tularensis LVS strains. We sequenced the entire plasmid and found six open reading frames (ORFs)-ORF1, ORF2, ORF3, ORF4, ORF5, and ORFm. ORF3,...

journal_title：Plasmid

authors： Pomerantsev AP,Golovliov IR,Ohara Y,Mokrievich AN,Obuchi M,Norqvist A,Kuoppa K,Pavlov VM

更新日期：2001-11-01 00:00:00

abstract：:Bacillus mycoides, a member of the Bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. Two plasmids, one from a strain curving to the right (pDx14.2),...

journal_title：Plasmid

authors： Di Franco C,Santini T,Pisaneschi G,Beccari E

更新日期：2005-11-01 00:00:00

abstract：:Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...

journal_title：Plasmid

authors： Mesas JM,Rodríguez MC,Alegre MT

更新日期：2001-09-01 00:00:00

abstract：:Acinetobacter baumannii is an important nosocomial pathogen that often complicates treatment because of its high level of resistance to antibiotics. Though plasmids can potentially introduce various genes into bacterial strains, compared to other Gram-negative bacteria, information about the unique A. baumannii plasmi...

journal_title：Plasmid

authors： Hamidian M,Ambrose SJ,Hall RM

更新日期：2016-01-01 00:00:00

abstract：:It is now known that conjugative pili are determined by representative plasmids for all incompatibility groups in Escherichia coli K-12. They fall into three basic morphological groups, which are described: thin flexible, thick flexible, and rigid filaments or rods. The main thrust of this study, however, has been the...

journal_title：Plasmid

authors： Bradley DE

更新日期：1980-09-01 00:00:00

abstract：:The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...

journal_title：Plasmid

authors： Guay GG,Khan SA,Rothstein DM

更新日期：1993-09-01 00:00:00

abstract：:Borrelia species comprise a unique genus of bacterial pathogens. These organisms contain a segmented genome with up to two dozen plasmids ranging in size from 5 kb up to about 200 kb. The plasmids have also been referred to as mini-chromosomes or essential genetic elements, as some of them carry information important ...

journal_title：Plasmid

authors： Chaconas G,Norris SJ

更新日期：2013-09-01 00:00:00

abstract：:The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...

journal_title：Plasmid

authors： Bartosik D,Baj J,Piechucka E,Waker E,Wlodarczyk M

更新日期：2002-09-01 00:00:00

abstract：:Transposon-insertion mutants with vir- Ti plasmids were characterized and then used in complementation experiments. One of the mutants (LBA 1517) had a mutation in a newly discovered vir locus called virF. The virF mutation led to a strongly diminished virulence on tomato and tobacco, but not on certain other plant sp...

journal_title：Plasmid

authors： Hooykaas PJ,Hofker M,den Dulk-Ras H,Schilperoort RA

更新日期：1984-05-01 00:00:00

abstract：:Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of ...

journal_title：Plasmid

authors： Valverde V,Duplan H,Knibiehler M,Masson JM

更新日期：1994-07-01 00:00:00

abstract：:Naturally occurring plasmids have medical importance given that they frequently code for virulence or antibiotic resistance. In many cases, plasmids impose a fitness cost to their hosts, meaning that the growth rate of plasmid-bearing cells is lower than that of plasmid-free cells. However, this does not fit with the ...

journal_title：Plasmid

authors： Gama JA,Zilhão R,Dionisio F

更新日期：2018-09-01 00:00:00

abstract：:A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...

journal_title：Plasmid

authors： Zhong Z,Caspi R,Mincer T,Helinski D,Knauf V,Boardman K,Wilkinson JE,Shea T,DeLoughery C,Toukdarian A

更新日期：2002-01-01 00:00:00