Sequence and analysis of the 46.6-kb plasmid pA1 from Sphingomonas sp. A1 that corresponds to the typical IncP-1beta plasmid backbone without any accessory gene.

abstract：:The promiscuous IncPα plasmids RK2 and R995 encode a broad-host-range partition system, whose essential components include the incC and korB genes and a DNA site (O(B)) to which the korB product binds. IncC2, the smaller of the two incC products, is sufficient for stabilization of R995ΔincC. It is a member of the type...

journal_title：Plasmid

authors： Siddique A,Figurski DH

更新日期：2012-09-01 00:00:00

abstract：:A suppressor of the mutations which inhibit the establishment of lysogeny in Pseudomonas aeruginosa has been found to be encoded by P. aeruginosa plasmids of four separate incompatibility groups. The wide distribution of this phenotype (designated Sly for suppressor of lysogeny establishment deficiency) indicates that...

journal_title：Plasmid

authors： Mondello FJ,Miller RV

更新日期：1984-03-01 00:00:00

abstract：:A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...

journal_title：Plasmid

authors： Menéndez C,Igloi GL,Brandsch R

更新日期：1997-01-01 00:00:00

abstract：:The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...

journal_title：Plasmid

authors： Osbourn SE,Turner AK,Grinsted J

更新日期：1995-01-01 00:00:00

abstract：:We report the characterization of Tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the R-plasmid pGD10. Tn602 is highly homologous to the previously characterized Tn903, present on the R-plasmid R6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-I (hom...

journal_title：Plasmid

authors： Stibitz S,Davies JE

更新日期：1987-05-01 00:00:00

abstract：:Escherichia coli pcnB gene was discovered as a regulator of ColE1 plasmid copy number about 20 years ago. Further studies indicated that this gene codes for poly(A) polymerase I (PAP I). However, although importance of RNA polyadenylation has been demonstrated and biochemical properties of PAP I have been studied exte...

journal_title：Plasmid

authors： Jasiecki J,Wegrzyn G

更新日期：2006-03-01 00:00:00

abstract：:Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...

journal_title：Plasmid

authors： Mitoma Y,Aoki T,Crosa JH

更新日期：1984-11-01 00:00:00

abstract：:We describe the construction of a series of shuttle vectors for Staphylococcus aureus. In order to determine transcriptional regulation by essential regulators, we constructed promoterless luxABCDE reporter system using a TetR-regulated antisense RNA expression vector, pJYJ909, which is composed of S. aureus plasmid p...

journal_title：Plasmid

authors： Yan M,Yu C,Yang J,Ji Y

更新日期：2009-05-01 00:00:00

abstract：:Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging fro...

journal_title：Plasmid

authors： Fornari CS,Watkins M,Kaplan S

更新日期：1984-01-01 00:00:00

abstract：:The replication (rep) regions of small plasmids from three Sinorhizobium meliloti strains were cloned by marker rescue. Two unique replication regions were identified, one of which was common to two different strains. Plasmid pBB83 carried a 7.2 kbp rep region from a 42 kbp plasmid, and pBB84 carried a 4.5 kbp rep reg...

journal_title：Plasmid

authors： Watson RJ,Heys R

更新日期：2006-03-01 00:00:00

abstract：:Tn1935, a 23.5-kb transposon mediating resistance to ampicillin, kanamycin, mercury, spectinomycin, and sulfonamide was isolated from pZM3, an IncFIme virulence plasmid from Salmonella wien. Tn1935 possesses the entire sequence of Tn21 and contains two additional DNA segments of 0.95 and 2.7 kb carrying the ampicillin...

journal_title：Plasmid

authors： Colonna B,Bernardini M,Micheli G,Maimone F,Nicoletti M,Casalino M

更新日期：1988-11-01 00:00:00

abstract：:The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that...

journal_title：Plasmid

authors： Chakravartty V,Cronan JE

更新日期：2015-09-01 00:00:00

abstract：:The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...

journal_title：Plasmid

authors： Song JY,Choi SH,Byun EY,Lee SG,Park YH,Park SG,Lee SK,Kim KM,Park JU,Kang HL,Baik SC,Lee WK,Cho MJ,Youn HS,Ko GH,Bae DW,Rhee KH

更新日期：2003-09-01 00:00:00

abstract：:We have evaluated the ability of immortalized human fibroblasts to recombine transfected plasmid DNA. A number of cell lines from normal individuals and from patients with DNA damage-processing defects were examined. Two plasmid recombination substrates were derived from pSV2neo and contained nonoverlapping deletions ...

journal_title：Plasmid

authors： Timme TL,Wood CM,Moses RE

更新日期：1989-07-01 00:00:00

abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...

journal_title：Plasmid

authors： Caillaud F,Carlier C,Courvalin P

更新日期：1987-01-01 00:00:00

abstract：:The sequences of three transfer RNAs from mosquito cell mitochondria, tRNAArgUCG, tRNAAspGUC, and tRNAIleGAU, determined using a combination of rapid ladder and fingerprinting procedures are reported. These were compared with hamster mitochondrial tRNAArgUCG and tRNAAspGUC determined similarly, and a bovine mitochondr...

journal_title：Plasmid

authors： HsuChen CC,Cleaves GR,Dubin DT

更新日期：1983-07-01 00:00:00

abstract：:A combined physical and functional map of plasmid RP4 is presented including the sites for 18 restriction endonucleases. Several cleavage sites of SphI, BalI, and ApaI are suitable for the dissection of the transfer gene regions. Recombinant plasmids containing RP4 SphI fragments were constructed to assist in localizi...

journal_title：Plasmid

authors： Lanka E,Lurz R,Fürste JP

更新日期：1983-11-01 00:00:00

abstract：:Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....

journal_title：Plasmid

authors： Dumans AT,Carvalho MF,Coelho A

更新日期：1989-11-01 00:00:00

abstract：:Expression vectors for industrial production should be stable and allow tight control of protein synthesis. This is necessary to ensure plasmid transmission to daughter cells in order to achieve a stable population capable of synthesizing high amounts of the target protein. A high-copy-number plasmid, pAE, was previou...

journal_title：Plasmid

authors： Eguia FAP,Ramos HR,Kraschowetz S,Omote D,Ramos CRR,Ho PL,Carvalho E,Gonçalves VM

更新日期：2018-06-01 00:00:00

abstract：:The gene(s) for chromosome-mediated beta-lactamase production of Proteus vulgaris GN7919 was cloned into a unique EcoRI site of pACYC184 as an insert of a 14.2-kb fragment, which was further digested into two fragments with EcoRI, 4.9 and 9.3 kb. The restriction enzyme digestion pattern of the recombinant plasmid, des...

journal_title：Plasmid

authors： Maejima T,Ohya Y,Mitsuhashi S,Inoue M

更新日期：1987-09-01 00:00:00

abstract：:Recently an insertional mutagenesis procedure has been developed to permit cloning of genes affected in developmental mutants of Dictyostelium discoideum (Kuspa and Loomis, Proc. Natl. Acad. Sci. USA 89, 8803-8807, 1992). In this procedure a plasmid bearing the URA (pyr5-6) gene is linearized with a restriction enzyme...

journal_title：Plasmid

authors： Chang WT,Gross JD,Newell PC

更新日期：1995-11-01 00:00:00

abstract：:The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...

journal_title：Plasmid

authors： Hefford MA,Teather RM,Forster RJ

更新日期：1993-01-01 00:00:00

abstract：:Escherichia coli strain K12 BM14, carrying plasmid R751 (51.4 kb; Tpr, Tra+, IncP), was used as donor in matings with reference strains of the six Brucella nomenspecies, not known so far to harbor naturally occurring plasmids. R751 was easily transferred to and between Brucella spp., at frequencies ranging between 10(...

journal_title：Plasmid

authors： Verger JM,Grayon M,Chaslus-Dancla E,Meurisse M,Lafont JP

更新日期：1993-03-01 00:00:00

abstract：:Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...

journal_title：Plasmid

authors： Mesas JM,Rodríguez MC,Alegre MT

更新日期：2001-09-01 00:00:00

abstract：:CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...

journal_title：Plasmid

authors： Kang N,Choi E,Kim SG,Hwang J

更新日期：2015-09-01 00:00:00

abstract：:A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...

journal_title：Plasmid

authors： Zhong Z,Caspi R,Mincer T,Helinski D,Knauf V,Boardman K,Wilkinson JE,Shea T,DeLoughery C,Toukdarian A

更新日期：2002-01-01 00:00:00

abstract：:The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...

journal_title：Plasmid

authors： Guay GG,Khan SA,Rothstein DM

更新日期：1993-09-01 00:00:00

abstract：:A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restrictio...

journal_title：Plasmid

authors： Phillips GJ

更新日期：1999-01-01 00:00:00

abstract：:Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...

journal_title：Plasmid

authors： Miller CA 3rd,Tan X,Wilson M,Bhattacharyya S,Ludwig S

更新日期：2010-03-01 00:00:00

abstract：:Almost 200 scientists from America, Europe, Asia, Australia, and Africa participated in the Plasmid Biology 2004 meeting, which was organized between 15th and 21st September 2004 in Kanoni (Corfu island), Greece. Various aspects of biology of plasmids and other mobile genetic elements were discussed during the meeting...

journal_title：Plasmid

authors： Wegrzyn G

更新日期：2005-01-01 00:00:00