Abstract:
:The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.
journal_name
Plasmidjournal_title
Plasmidauthors
Chakravartty V,Cronan JEdoi
10.1016/j.plasmid.2015.03.001subject
Has Abstractpub_date
2015-09-01 00:00:00pages
21-6eissn
0147-619Xissn
1095-9890pii
S0147-619X(15)00019-0journal_volume
81pub_type
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