Abstract:
:Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available.
journal_name
Plasmidjournal_title
Plasmidauthors
Scott HN,Laible PD,Hanson DKdoi
10.1016/s0147-619x(03)00030-1keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
74-9issue
1eissn
0147-619Xissn
1095-9890pii
S0147619X03000301journal_volume
50pub_type
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