Abstract：
:Almost 200 scientists from America, Europe, Asia, Australia, and Africa participated in the Plasmid Biology 2004 meeting, which was organized between 15th and 21st September 2004 in Kanoni (Corfu island), Greece. Various aspects of biology of plasmids and other mobile genetic elements were discussed during the meeting, including problems of replication, transfer, stable inheritance, and evolution. Medical and veterinary aspects of plasmids were highlighted as well as other applications of these replicons. It appears that plasmids and other mobile genetic elements are still excellent models in studies of basic biological problems at the molecular level, and their role in medicine and genetic engineering can be enormous. Moreover, studies on ecology of plasmids provide extremely important data that can be used in environment protection as well as in biotechnology. Understanding the importance of studies on plasmids and other mobile genetic elements, participants of the meeting decided to establish the International Society for Plasmid Biology.
journal_name
Plasmidjournal_title
Plasmidauthors
Wegrzyn Gdoi
10.1016/j.plasmid.2004.10.002keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
14-22issue
1eissn
0147-619Xissn
1095-9890pii
S0147-619X(04)00121-0journal_volume
53pub_type
相关文献
PLASMID文献大全abstract：:Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1999.1412
更新日期：1999-09-01 00:00:00
abstract：:The plasmid pMSP3535 is a popular vector for nisin-inducible expression of heterologous genes in lactic acid bacteria. However, the maximum protein expression level achievable with pMSP3535 is relatively low. In an effort to increase expression we modified pMSP3535 to create a high expression variant termed pMSP3535H2...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2007.05.004
更新日期：2007-11-01 00:00:00
abstract：:The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1999.1399
更新日期：1999-05-01 00:00:00
abstract：:The replication (rep) regions of small plasmids from three Sinorhizobium meliloti strains were cloned by marker rescue. Two unique replication regions were identified, one of which was common to two different strains. Plasmid pBB83 carried a 7.2 kbp rep region from a 42 kbp plasmid, and pBB84 carried a 4.5 kbp rep reg...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2005.08.003
更新日期：2006-03-01 00:00:00
abstract：:pSAM2 is an 11-kb plasmid integrated in the Streptomyces ambofaciens ATCC23877 and ATCC15154 genomes and found additionally as a free replicon in an uv derivative. After transfer into S. ambofaciens DSM40697 (devoid of pSAM2) or into Streptomyces lividans, specific integration of pSAM2 occurred very efficiently. A 58-...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(89)90087-5
更新日期：1989-01-01 00:00:00
abstract：:A combined physical and functional map of plasmid RP4 is presented including the sites for 18 restriction endonucleases. Several cleavage sites of SphI, BalI, and ApaI are suitable for the dissection of the transfer gene regions. Recombinant plasmids containing RP4 SphI fragments were constructed to assist in localizi...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(83)90047-1
更新日期：1983-11-01 00:00:00
abstract：:The effects of the single-strand origins (SSOs) of plasmid pMV158 on (i) the conversion of its single-stranded (ss) replication intermediates to double-stranded (ds) plasmid DNA and (ii) its maintenance were analyzed. The rolling-circle plasmid pMV158, which replicates via ssDNA intermediates, contains two single-stra...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1995.1010
更新日期：1995-03-01 00:00:00
abstract：:A novel type I restriction-modification specificity subunit, S. LlaW12I, has been identified on the naturally occurring 8.0-kb plasmid pAW122 in the lactic acid bacterium Lactococcus lactis subsp. cremoris W12. Presence of the HsdS protein together with a complete type I restriction-modification system conferred incre...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.2000.1478
更新日期：2000-09-01 00:00:00
abstract：:The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/s0147-619x(03)00059-3
更新日期：2003-09-01 00:00:00
abstract：:As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2015.10.001
更新日期：2015-11-01 00:00:00
abstract：:By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sens...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(83)90045-8
更新日期：1983-11-01 00:00:00
abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(87)90009-6
更新日期：1987-01-01 00:00:00
abstract：:The Lactococcus lactis group II intron (Ll.ltrB) retrohomes into the ltrB gene at high efficiency. To date, the critical DNA bases recognized in vivo by the Ll.ltrB ribonucleoprotein (RNP) have been exclusively elucidated in Escherichia coli. However, recent evidence indicates host-dependant differences in Ll.ltrB mob...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2007.02.003
更新日期：2007-09-01 00:00:00
abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1062
更新日期：1994-09-01 00:00:00
abstract：:Nucleotide sequence analysis of the Tn4451-deletion derivatives, pJIR47 and pJIR86, which were derived from Escherichia coli and Clostridium perfringens, respectively, showed that the deletion events that led to the formation of these plasmids were identical and precise. The results also showed that the termini of thi...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(88)90055-8
更新日期：1988-03-01 00:00:00
abstract：:The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(89)90007-3
更新日期：1989-11-01 00:00:00
abstract：:A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.2001.1550
更新日期：2002-01-01 00:00:00
abstract：:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(91)90002-e
更新日期：1991-01-01 00:00:00
abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(85)90058-7
更新日期：1985-01-01 00:00:00
abstract：:A restriction enzyme map of the IncFI plasmid ColV2-K94 was generated using EcoRI, BamHI, HindIII, and XhoI; the genetic features of this element were then mapped from previous heteroduplex studies. ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(85)90046-0
更新日期：1985-05-01 00:00:00
abstract：:The chimeric plasmid pBN183 was first constructed in Escherichia coli by ligating the BamHI-digested E. coli plasmid pBR322 and a Bg/II-linearized streptococcal plasmid, pNZ18. The pBN183 transformed E. coli to ApR at a frequency of (8.2 +/- 1.2) x 10(5) colony forming units (CFU)/microgram DNA. Electrotransformation ...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(92)90033-7
更新日期：1992-07-01 00:00:00
abstract：:Naturally occurring plasmids have medical importance given that they frequently code for virulence or antibiotic resistance. In many cases, plasmids impose a fitness cost to their hosts, meaning that the growth rate of plasmid-bearing cells is lower than that of plasmid-free cells. However, this does not fit with the ...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2018.09.009
更新日期：2018-09-01 00:00:00
abstract：:The promiscuous IncPα plasmids RK2 and R995 encode a broad-host-range partition system, whose essential components include the incC and korB genes and a DNA site (O(B)) to which the korB product binds. IncC2, the smaller of the two incC products, is sufficient for stabilization of R995ΔincC. It is a member of the type...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2012.04.003
更新日期：2012-09-01 00:00:00
abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(91)90042-u
更新日期：1991-11-01 00:00:00
abstract：:Fusobacterium nucleatum is a Gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. Genomic analyses of F. nucleatum indicate considerable genetic diversity and a prominent role for horizont...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2009.09.001
更新日期：2010-01-01 00:00:00
abstract：:We have constructed a pUC19-based multipurpose ATG vector in Schizosaccharomyces pombe with higher copy number and mitotic stability possible with commonly used vectors. The vector, having an NdeI site in its polylinker to provide ATG site for expression, carries a greatly truncated version of URA3 gene, URA3m, of Sac...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2012.07.001
更新日期：2012-11-01 00:00:00
abstract：:We report the mobilization by cointegration of the gonococcal 5.2 kb beta-lactamase plasmid pSJ5.2 in an Escherichia coli background. Transfer of pSJ5.2 was measured by filter mating assays with five different conjugative plasmids from Enterobacteriaceae and the gonococcal 41 kb tet(M). Plasmid pSJ5.2 was mobilized to...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/j.plasmid.2006.07.006
更新日期：2007-03-01 00:00:00
abstract：:Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...
journal_title：Plasmid
pub_type： 杂志文章,评审
doi：10.1016/j.plasmid.2014.05.004
更新日期：2014-07-01 00:00:00
abstract：:We have cloned and characterized an insertion sequence from Leuconostoc mesenteroides subsp. cremoris strain DB1165. This element, designated IS1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. The copy nu...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1016/0147-619x(92)90022-3
更新日期：1992-05-01 00:00:00
abstract：:Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...
journal_title：Plasmid
pub_type： 杂志文章
doi：10.1006/plas.1994.1029
更新日期：1994-05-01 00:00:00
