Characterization of pPT23B, the plasmid involved in syringolide production by Pseudomonas syringae pv. tomato PT23.

abstract：:Pseudomonas putida is a highly attractive production system for industrial needs. However, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a plasmid expressing a small RNA-based system with the potential to be used for di...

journal_title：Plasmid

authors： Apura P,Saramago M,Peregrina A,Viegas SC,Carvalho SM,Saraiva LM,Arraiano CM,Domingues S

更新日期：2020-05-01 00:00:00

abstract：:Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...

journal_title：Plasmid

authors： Yang X,Daniell H,McFadden B

更新日期：1994-09-01 00:00:00

abstract：:A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-d...

journal_title：Plasmid

authors： Projan SJ,Carleton S,Novick RP

更新日期：1983-03-01 00:00:00

abstract：:pHH6000 is a composite replicon made by the in vitro ligation of the IncP plasmid RP4 to a fragment of bacteriophage lambda capable of autonomous replication. Derivatives were selected in which it had integrated into the Escherichia coli chromosome by homologous recombination with the resident lambda prophage, and pla...

journal_title：Plasmid

authors： Grinter NJ

更新日期：1984-01-01 00:00:00

abstract：:Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...

journal_title：Plasmid

authors： Peters M,Tomikas A,Nurk A

更新日期：2004-11-01 00:00:00

abstract：:This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...

journal_title：Plasmid

authors： Khan SA

更新日期：2005-03-01 00:00:00

abstract：:Recently the plasmid RK2 replication initiation protein, TrfA, has been shown to bind to the beta subunit of DNA Polymerase III (DnaN) via a short pentapeptide with the consensus QL[S/D]LF. A second consensus peptide, the hexapeptide QLxLxL, has also been demonstrated to mediate binding to DnaN. Here we describe the r...

journal_title：Plasmid

authors： Dalrymple BP,Kongsuwan K,Wijffels G

更新日期：2007-01-01 00:00:00

abstract：:Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...

journal_title：Plasmid

authors： Manganelli R,Romano L,Ricci S,Zazzi M,Pozzi G

更新日期：1995-07-01 00:00:00

abstract：:Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...

journal_title：Plasmid

authors： Feng X,Ou LT,Ogram A

更新日期：1997-01-01 00:00:00

abstract：:The nucleotide sequence of a smallest cryptic plasmid pRK10 of Serratia marcescens ACE2 was determined. When compared to the all other plasmids reported so far from S. marcescens in sizes of over 70kb, pRK10 is only 4241bp long with 53% G+C content and has five coding sequences representing a coding percentage of 65.4...

journal_title：Plasmid

authors： Syed Ibrahim K,Bakkiyaraj D,James R,Ganesh Babu T,Karutha Pandian ST

更新日期：2009-11-01 00:00:00

abstract：:Temperate bacteriophage MAV1 is found in certain highly virulent strains of Mycoplasma arthritidis. Integration sites, portions of the right and left prophage ends, and flanking DNA from eight prophages in seven M. arthritidis strains were characterized in this study. attb and attp sites conformed for the most part to...

journal_title：Plasmid

authors： Washburn LR,Miller EJ,Mukherjee S,Dannenbring D

更新日期：2004-07-01 00:00:00

abstract：:CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...

journal_title：Plasmid

authors： Kang N,Choi E,Kim SG,Hwang J

更新日期：2015-09-01 00:00:00

abstract：:Two B/O plasmids were sequenced. The kanamycin resistance plasmid R805a was found in a Salmonella Typhi strain from a 1972 typhoid fever outbreak in Mexico City. pCERC6, which confers resistance to ampicillin, streptomycin and sulphamethoxazole, was found in a commensal E. coli isolated from a healthy adult in Sydney ...

journal_title：Plasmid

authors： Moran RA,Richardson IA,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restrictio...

journal_title：Plasmid

authors： Phillips GJ

更新日期：1999-01-01 00:00:00

abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...

journal_title：Plasmid

authors： Yamamoto T,Hanawa T,Murayama SY,Ogata S

更新日期：1994-09-01 00:00:00

abstract：:The mercury(II)-resistance transposons Tn502 and Tn512 were sequenced and shown to be members of the Tn5053 family. They are currently the sole representatives from the clinical setting and were obtained from geographically disparate Pseudomonas aeruginosa strains. The family is comprised of six novel transposons that...

journal_title：Plasmid

authors： Petrovski S,Blackmore DW,Jackson KL,Stanisich VA

更新日期：2011-01-01 00:00:00

abstract：:Nocardia brasiliensis, is a bacteria that lives as saprophyte in soil and causes a disease called actinomycetoma in both human and animals. Nocardia brasiliensis is an intracellular, facultative bacterium that replicates and survives within host macrophages. The mechanisms involved in the evasion of the microbicidal a...

journal_title：Plasmid

authors： Salinas-Carmona MC,Rocha-Pizaña MR

更新日期：2011-01-01 00:00:00

abstract：:RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...

journal_title：Plasmid

authors： Sotirova VN,Calciano MA,Krueger W,Lalande M

更新日期：2006-05-01 00:00:00

abstract：:Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...

journal_title：Plasmid

authors： Strunnikov AV

更新日期：2006-03-01 00:00:00

abstract：:Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...

journal_title：Plasmid

authors： Bahk JD,Kioka N,Sakai H,Komano T

更新日期：1988-11-01 00:00:00

abstract：:pBTK445 is a newly described large (∼60Kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph Advenella kashmirensis. Based on its minimal replication region, a shuttle vector, pBTKS was constructed which can be used for diverse Alcaligenaceae members. The construct was found to be stab...

journal_title：Plasmid

authors： Dam B

更新日期：2011-03-01 00:00:00

abstract：:The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their ...

journal_title：Plasmid

authors： Davey RB,Bird PI,Nikoletti SM,Praszkier J,Pittard J

更新日期：1984-05-01 00:00:00

abstract：:The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...

journal_title：Plasmid

authors： Song JY,Choi SH,Byun EY,Lee SG,Park YH,Park SG,Lee SK,Kim KM,Park JU,Kang HL,Baik SC,Lee WK,Cho MJ,Youn HS,Ko GH,Bae DW,Rhee KH

更新日期：2003-09-01 00:00:00

abstract：:Pseudomonas syringae pv. phaseolicola (Pph) strain 1302A, a causative agent of halo blight in the common bean Phaseolus vulgaris, contains four native plasmids designated pAV505 (150 kb), pAV506 (50 kb), pAV507 (47 kb) and pAV508 (42 kb). Pph 1302A also contains a 106 kb genomic island PPHGI-1 which shares features wi...

journal_title：Plasmid

authors： Neale HC,Slater RT,Mayne LM,Manoharan B,Arnold DL

更新日期：2013-11-01 00:00:00

abstract：:A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...

journal_title：Plasmid

authors： Qiao M,Gao C,Cai B,Zhang X,Wang S

更新日期：1992-05-01 00:00:00

abstract：:Two independently isolated temperature-sensitive bacteriophage that are specific for enterobacterial hosts harboring HI and HII plasmids were characterized to determine if any identifiable differences existed between them. The traits examined included adsorption pattern of phage to H pili, bacteriophage size, sensitiv...

journal_title：Plasmid

authors： Maher D,Sherburne R,Taylor DE

更新日期：1991-09-01 00:00:00

abstract：:Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...

journal_title：Plasmid

authors： Mitoma Y,Aoki T,Crosa JH

更新日期：1984-11-01 00:00:00

abstract：:The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...

journal_title：Plasmid

authors： Osbourn SE,Turner AK,Grinsted J

更新日期：1995-01-01 00:00:00

abstract：:A new class of binary vectors has been constructed, containing the origin of replication of the W-type plasmid Sa. These vectors are designed to express foreign genes in plants under control of the TR gene 2' promoter or the promoter of a light-inducible ribulose-1,5-bisphosphate carboxylase small subunit gene of Arab...

journal_title：Plasmid

authors： Alliotte T,Zhu LH,Van Montagu M,Inzé D

更新日期：1988-05-01 00:00:00

abstract：:A phagemid vector library for cloning DNA with four nucleotide 5' or 3' overhangs has been constructed. This library is based on the pT7T3 vector (Pharmacia) which is a modification of the phagemid pTZ18U vector. We have chosen pT7T3 as the parent vector because it can be used for Sanger's dideoxy sequencing and for t...

journal_title：Plasmid

authors： Waye MM,Mui F,Hodge K,Li VK

更新日期：1991-07-01 00:00:00