Tailor-made sRNAs: a plasmid tool to control the expression of target mRNAs in Pseudomonas putida.

abstract：:Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...

journal_title：Plasmid

authors： Miller CA 3rd,Tan X,Wilson M,Bhattacharyya S,Ludwig S

更新日期：2010-03-01 00:00:00

abstract：:By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sens...

journal_title：Plasmid

authors： Maas R

更新日期：1983-11-01 00:00:00

abstract：:The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...

journal_title：Plasmid

authors： Guay GG,Khan SA,Rothstein DM

更新日期：1993-09-01 00:00:00

abstract：:Functional analysis of cloned genes often makes use of complementation after introducing these genes into cells of a mutant strain. Problems with this self-cloning step in the cyanobacterium Anacystis nidulans R2 have been encountered, which were mainly due to recombinational instability of gene and vector after trans...

journal_title：Plasmid

authors： Kuhlemeier CJ,Hardon EM,van Arkel GA,van de Vate C

更新日期：1985-11-01 00:00:00

abstract：:The effects of the single-strand origins (SSOs) of plasmid pMV158 on (i) the conversion of its single-stranded (ss) replication intermediates to double-stranded (ds) plasmid DNA and (ii) its maintenance were analyzed. The rolling-circle plasmid pMV158, which replicates via ssDNA intermediates, contains two single-stra...

journal_title：Plasmid

authors： Meijer WJ,van der Lelie D,Venema G,Bron S

更新日期：1995-03-01 00:00:00

abstract：:Expression vectors for industrial production should be stable and allow tight control of protein synthesis. This is necessary to ensure plasmid transmission to daughter cells in order to achieve a stable population capable of synthesizing high amounts of the target protein. A high-copy-number plasmid, pAE, was previou...

journal_title：Plasmid

authors： Eguia FAP,Ramos HR,Kraschowetz S,Omote D,Ramos CRR,Ho PL,Carvalho E,Gonçalves VM

更新日期：2018-06-01 00:00:00

abstract：:The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their ...

journal_title：Plasmid

authors： Davey RB,Bird PI,Nikoletti SM,Praszkier J,Pittard J

更新日期：1984-05-01 00:00:00

abstract：:pSAM2 is an 11-kb plasmid integrated in the Streptomyces ambofaciens ATCC23877 and ATCC15154 genomes and found additionally as a free replicon in an uv derivative. After transfer into S. ambofaciens DSM40697 (devoid of pSAM2) or into Streptomyces lividans, specific integration of pSAM2 occurred very efficiently. A 58-...

journal_title：Plasmid

authors： Boccard F,Smokvina T,Pernodet JL,Friedmann A,Guérineau M

更新日期：1989-01-01 00:00:00

abstract：:Homology was previously detected between the DNA restriction fragments containing Rhizobium meliloti nodulation genes and the 90-MDa plasmid, p90, of Azospirillum brasilense Sp7. Two DNA loci from Sp7 genome that complement mutations in the exopolysaccharide synthesis genes, exoB and exoC, of R. meliloti were also sho...

journal_title：Plasmid

authors： Onyeocha I,Vieille C,Zimmer W,Baca BE,Flores M,Palacios R,Elmerich C

更新日期：1990-05-01 00:00:00

abstract：:Plasmid pT181 is a small multicopy plasmid from Staphylococcus aureus that belongs to incompatibility group 3 and expresses two distinct types of incompatibility, Inc3A and Inc3B. Inc3A incompatibility is expressed by the primary replication control determinant, copA, which specifies two small transcripts, RNA I and R...

journal_title：Plasmid

authors： Highlander SK,Novick RP

更新日期：1990-01-01 00:00:00

abstract：:Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...

journal_title：Plasmid

authors： Feng X,Ou LT,Ogram A

更新日期：1997-01-01 00:00:00

abstract：:A cell clone (cl.2) having an atypical transformed morphology was isolated from a murine C127 cell culture experimentally infected with a bovine papillomavirus type 1 (BPV-1) virion preparation. cl.2 cells exhibited minimal transformed characteristics and contained multiple copies of a BPV-1 plasmid with a molecular s...

journal_title：Plasmid

authors： Burnett S,Moreno-Lopez J,Pettersson U

更新日期：1988-07-01 00:00:00

abstract：:Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence ...

journal_title：Plasmid

authors： Tabata A,Deutsch D,Otsuka S,Verratti K,Tomoyasu T,Nagamune H,Fischetti VA

更新日期：2018-01-01 00:00:00

abstract：:DNA replication initiation has been well-characterized; however, studies in the past few years have shown that there are still important discoveries to be made. Recent publications concerning the bacterial DnaA protein have revealed how this replication initiator, via interaction with specific sequences within the ori...

journal_title：Plasmid

authors： Zabrocka E,Wegrzyn K,Konieczny I

更新日期：2014-11-01 00:00:00

abstract：:A plasmid vector for cloning in Bacillus sphaericus 1593 was constructed in B. subtilis from two parent plasmids, pBC16 and pBD64. When characterized, the 3.9-MDa chimeric plasmid pNN101 was found to consist of the MspI fragment containing the chloramphenicol acetyltransferase (CAT) gene from pBD64 inserted into an Ms...

journal_title：Plasmid

authors： Norton NB,Orzech KA,Burke WF Jr

更新日期：1985-05-01 00:00:00

abstract：:Bacterial plasmids and phages encode the synthesis of toxic molecules that inhibit protozoan predation. One such toxic molecule is violacein, a purple pigmented, anti-tumour antibiotic produced by the Gram-negative soil bacterium Chromobacterium violaceum. In the current experiments a range of Escherichia coli K12 str...

journal_title：Plasmid

authors： Ahmetagic A,Philip DS,Sarovich DS,Kluver DW,Pemberton JM

更新日期：2011-09-01 00:00:00

abstract：:Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....

journal_title：Plasmid

authors： Dumans AT,Carvalho MF,Coelho A

更新日期：1989-11-01 00:00:00

abstract：:Lactococcus lactis ME2 can transfer a 46-kb plasmid, pTR2030, which encodes abortive phage infection (Hsp) and restriction/modification (R/M) activities. pTR2030 can be detected as a monomeric plasmid in transconjugants at low copy number, but not in ME2. pTR2030-specific probes were cloned and used to determine the l...

journal_title：Plasmid

authors： Hill C,Miller LA,Klaenhammer TR

更新日期：1991-03-01 00:00:00

abstract：:We have constructed shuttle vectors for integration of genes via double homologous recombination into three ectopic sites on the chromosome of Bacillus subtilis. The sites of integration are the pyrD, gltA, and sacA genes located at 139 degrees, 172 degrees, and 333 degrees, respectively, on the chromosome. Integratio...

journal_title：Plasmid

authors： Middleton R,Hofmeister A

更新日期：2004-05-01 00:00:00

abstract：:The incompatibility region of the Streptomyces plasmid pSL1 was identified as a 240-bp segment, though some other function from the vector plasmid was also necessary. A 540-bp fragment including the 240-bp region was enough for full expression of incompatibility. Inserted mutation analysis led to a more detailed locat...

journal_title：Plasmid

authors： Urabe H,Shindoh Y,Nakano MM,Ogawara H

更新日期：1987-03-01 00:00:00

abstract：:Cloning vectors (pFD1001, pFD1192, pFD1194, and pFD1212) were constructed by extension of the host range of a 7.2-kb Rhizobium meliloti cryptic plasmid (pRM1132f) with the ColE1-based plasmids, pBR322, pACYC177, pACYC 184, pSUP301, or pHC179; mobilization was facilitated by introduction of the oriT region from pRK2, a...

journal_title：Plasmid

authors： Froissard D,Bromfield ES,Whitwill S,Barran LR

更新日期：1995-05-01 00:00:00

abstract：:Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...

journal_title：Plasmid

authors： Manganelli R,Romano L,Ricci S,Zazzi M,Pozzi G

更新日期：1995-07-01 00:00:00

abstract：:We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb E...

journal_title：Plasmid

authors： Kawaguchi R,Nagaoka T,Burgess JG,Takeyama H,Matsunaga T

更新日期：1994-11-01 00:00:00

abstract：:The large, 350-kb Sym (symbiotic) plasmid pRjaUSDA193 of Rhizobium fredii was examined to determine the frequency of repeated sequences present and to produce a physical and genetic map of a large region of the plasmid. A novel hybridization method, the Southern Cross, revealed that the plasmid pRjaUSDA193 contained m...

journal_title：Plasmid

authors： Masterson RV,Atherly AG

更新日期：1986-07-01 00:00:00

abstract：:Temperate bacteriophage MAV1 is found in certain highly virulent strains of Mycoplasma arthritidis. Integration sites, portions of the right and left prophage ends, and flanking DNA from eight prophages in seven M. arthritidis strains were characterized in this study. attb and attp sites conformed for the most part to...

journal_title：Plasmid

authors： Washburn LR,Miller EJ,Mukherjee S,Dannenbring D

更新日期：2004-07-01 00:00:00

abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...

journal_title：Plasmid

authors： Yamamoto T,Hanawa T,Murayama SY,Ogata S

更新日期：1994-09-01 00:00:00

abstract：:Escherichia coli strain K12 BM14, carrying plasmid R751 (51.4 kb; Tpr, Tra+, IncP), was used as donor in matings with reference strains of the six Brucella nomenspecies, not known so far to harbor naturally occurring plasmids. R751 was easily transferred to and between Brucella spp., at frequencies ranging between 10(...

journal_title：Plasmid

authors： Verger JM,Grayon M,Chaslus-Dancla E,Meurisse M,Lafont JP

更新日期：1993-03-01 00:00:00

abstract：:The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that...

journal_title：Plasmid

authors： Chakravartty V,Cronan JE

更新日期：2015-09-01 00:00:00

abstract：:Fusobacterium nucleatum is a Gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. Genomic analyses of F. nucleatum indicate considerable genetic diversity and a prominent role for horizont...

journal_title：Plasmid

authors： Claypool BM,Yoder SC,Citron DM,Finegold SM,Goldstein EJ,Haake SK

更新日期：2010-01-01 00:00:00

abstract：:The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the genetically more studied linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the help of an ordered co...

journal_title：Plasmid

authors： Redenbach M,Bibb M,Gust B,Seitz B,Spychaj A

更新日期：1999-11-01 00:00:00