Abstract:
:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the production of a set of stress proteins; which are also induced by heat shock (Yamamoto et al., 1994, Microbiol. Immunol. 38, 295-300). To examine the role of bacterial stress proteins in the adaptation to environmental changes encountered during infectious processes, we have isolated stress-sensitive mutants from Y. enterocolitica in which mini-Tn10 transposon insertions allow bacterial growth at 28 degrees C but prevent growth at an elevated temperature, 39 degrees C. Eight independent insertions were obtained and preliminarily characterized by Southern blot hybridization and morphological analysis.
journal_name
Plasmidjournal_title
Plasmidauthors
Yamamoto T,Hanawa T,Murayama SY,Ogata Sdoi
10.1006/plas.1994.1062subject
Has Abstractpub_date
1994-09-01 00:00:00pages
238-43issue
2eissn
0147-619Xissn
1095-9890pii
S0147-619X(84)71062-6journal_volume
32pub_type
杂志文章相关文献
PLASMID文献大全abstract::A new insertion variant of IS231 family, designated ISBth4, was identified from Bacillus thuringiensis MEX312. ISBth4 is 2046bp in length and is delimited by two 17bp inverted repeats (IR) with one mismatch, flanked by two perfect 11bp direct repeats (DR). ISBth4 contains two open reading frames (ORFs), ORF1 and ORF2,...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.10.002
更新日期:2010-01-01 00:00:00
abstract::The gene(s) for chromosome-mediated beta-lactamase production of Proteus vulgaris GN7919 was cloned into a unique EcoRI site of pACYC184 as an insert of a 14.2-kb fragment, which was further digested into two fragments with EcoRI, 4.9 and 9.3 kb. The restriction enzyme digestion pattern of the recombinant plasmid, des...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90039-4
更新日期:1987-09-01 00:00:00
abstract::Previous explanations of why bacterial genes for certain "optional" traits tend to occur on plasmids rather than chromosomes are based on an outdated misunderstanding of natural selection. They also fail to explain why certain characters that are ubiquitous in some bacterial species tend to occur on plasmids. This pap...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/0147-619x(89)90040-1
更新日期:1989-05-01 00:00:00
abstract::pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(91)90042-u
更新日期:1991-11-01 00:00:00
abstract::Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1013
更新日期:1994-03-01 00:00:00
abstract::The region responsible for the maintenance of the prophage of bacteriophage P7 as a stable, unit-copy plasmid was isolated in a lambda att vector which lysogenizes Escherichia coli as a stable unit-copy plasmid under the control of the P7 replication origin. The P7 plasmid-maintenance region was shown to consist of ad...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90083-7
更新日期:1987-07-01 00:00:00
abstract::Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90035-b
更新日期:1990-03-01 00:00:00
abstract::The nucleotide sequence of two novel plasmids isolated from the extreme thermophilic anaerobic bacterium Anaerocellum thermophilum DSM6725 (A. thermophilum), growing optimally at 70 degrees C, has been determined. pBAS2 was found to be a 3653 bp plasmid with a GC content of 43%, and the sequence revealed 10 open readi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.06.001
更新日期:2004-09-01 00:00:00
abstract::Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1055
更新日期:1994-09-01 00:00:00
abstract::A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimat...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1074
更新日期:1994-11-01 00:00:00
abstract::The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1054
更新日期:1993-11-01 00:00:00
abstract::The stable maintenance of certain plasmids in bacterial populations has contributed significantly to the current worldwide antibiotic resistance (AbR) emergency. IncX plasmids, long underestimated in this regard, have achieved recent notoriety for their roles in transmission of resistance to carbapenem and colistin, t...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2017.02.006
更新日期:2017-05-01 00:00:00
abstract::The chimeric plasmid pBN183 was first constructed in Escherichia coli by ligating the BamHI-digested E. coli plasmid pBR322 and a Bg/II-linearized streptococcal plasmid, pNZ18. The pBN183 transformed E. coli to ApR at a frequency of (8.2 +/- 1.2) x 10(5) colony forming units (CFU)/microgram DNA. Electrotransformation ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90033-7
更新日期:1992-07-01 00:00:00
abstract::An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1998.1366
更新日期:1998-11-01 00:00:00
abstract::Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2002.1565
更新日期:2002-03-01 00:00:00
abstract::Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2005.08.004
更新日期:2006-03-01 00:00:00
abstract::pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.07.003
更新日期:2015-09-01 00:00:00
abstract::Two distinct families of repetitive DNA elements (1.4 and 1.2 kb) were identified from S1 nuclease-treated genomic DNA of four strains of Thiobacillus ferrooxidans. The 1.4-kb fragment hybridized with IST2, an insertion sequence of T. ferrooxidans. The 1.2-kb fragment was cloned and sequenced. The sequence (IST445), 1...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1306
更新日期:1997-01-01 00:00:00
abstract::The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.03.001
更新日期:2015-09-01 00:00:00
abstract::Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90013-3
更新日期:1990-09-01 00:00:00
abstract::Two independently isolated temperature-sensitive bacteriophage that are specific for enterobacterial hosts harboring HI and HII plasmids were characterized to determine if any identifiable differences existed between them. The traits examined included adsorption pattern of phage to H pili, bacteriophage size, sensitiv...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(91)90055-2
更新日期:1991-09-01 00:00:00
abstract::Transformation of Escherichia coli with Dictyostelium discoideum genomic DNA containing integrated shuttle vectors in multicopy, tandemly duplicated format resulted in the establishment of the linear plasmid molecules as circular monomeric replicons. The transformation efficiencies were comparable to those obtained wi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0036
更新日期:1996-09-01 00:00:00
abstract::We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90069-0
更新日期:1983-09-01 00:00:00
abstract::Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1029
更新日期:1994-05-01 00:00:00
abstract::We describe the construction of a series of shuttle vectors for Staphylococcus aureus. In order to determine transcriptional regulation by essential regulators, we constructed promoterless luxABCDE reporter system using a TetR-regulated antisense RNA expression vector, pJYJ909, which is composed of S. aureus plasmid p...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.02.001
更新日期:2009-05-01 00:00:00
abstract::To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2008.09.006
更新日期:2009-01-01 00:00:00
abstract::We have cloned and characterized an insertion sequence from Leuconostoc mesenteroides subsp. cremoris strain DB1165. This element, designated IS1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. The copy nu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90022-3
更新日期:1992-05-01 00:00:00
abstract::The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1045
更新日期:1993-09-01 00:00:00
abstract::Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1467
更新日期:2000-05-01 00:00:00
abstract::Bacillus coagulans is a sporogenic lactic acid bacterium that ferments glucose and xylose, major components of plant biomass, a potential feedstock for cellulosic ethanol. The temperature and pH for optimum rate of growth of B. coagulans (50 to 55 degrees C, pH 5.0) are very similar to that of commercially developed f...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.11.006
更新日期:2007-07-01 00:00:00