The plasmid-maintenance functions of the P7 prophage.

abstract：:The gene(s) for chromosome-mediated beta-lactamase production of Proteus vulgaris GN7919 was cloned into a unique EcoRI site of pACYC184 as an insert of a 14.2-kb fragment, which was further digested into two fragments with EcoRI, 4.9 and 9.3 kb. The restriction enzyme digestion pattern of the recombinant plasmid, des...

journal_title：Plasmid

authors： Maejima T,Ohya Y,Mitsuhashi S,Inoue M

更新日期：1987-09-01 00:00:00

abstract：:Plasmid pT181 is a small multicopy plasmid from Staphylococcus aureus that belongs to incompatibility group 3 and expresses two distinct types of incompatibility, Inc3A and Inc3B. Inc3A incompatibility is expressed by the primary replication control determinant, copA, which specifies two small transcripts, RNA I and R...

journal_title：Plasmid

authors： Highlander SK,Novick RP

更新日期：1990-01-01 00:00:00

abstract：:Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjug...

journal_title：Plasmid

authors： Dunn AK,Martin MO,Stabb EV

更新日期：2005-09-01 00:00:00

abstract：:A chimeric plasmid, pRS7Rep (6.1 kb), was constructed using the replication region of pRS7, a large plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli using its pIJ2925 componen...

journal_title：Plasmid

authors： Rodríguez MC,Alegre MT,Martín MC,Mesas JM

更新日期：2015-01-01 00:00:00

abstract：:Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...

journal_title：Plasmid

authors： Peters M,Tomikas A,Nurk A

更新日期：2004-11-01 00:00:00

abstract：:We have modified an E. coli-staphylococcal shuttle vector for use in the general cloning and expression of genes from pathogenic staphylococci in Staphylococcus carnosus. As S. carnosus is non-pathogenic, this expression system will facilitate the study of the roles of individual gene products in the disease process. ...

journal_title：Plasmid

authors： Williams RJ,Nair SP,Henderson B,Holland KT,Ward JM

更新日期：2002-05-01 00:00:00

abstract：:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...

journal_title：Plasmid

authors： Wabiko H,Kagaya M,Sano H

更新日期：1991-01-01 00:00:00

abstract：:The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...

journal_title：Plasmid

authors： Song JY,Choi SH,Byun EY,Lee SG,Park YH,Park SG,Lee SK,Kim KM,Park JU,Kang HL,Baik SC,Lee WK,Cho MJ,Youn HS,Ko GH,Bae DW,Rhee KH

更新日期：2003-09-01 00:00:00

abstract：:To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...

journal_title：Plasmid

authors： Domingues E,Brillet T,Vasseur C,Agier V,Marden MC,Baudin-Creuza V

更新日期：2009-01-01 00:00:00

abstract：:A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...

journal_title：Plasmid

authors： Zhong Z,Caspi R,Mincer T,Helinski D,Knauf V,Boardman K,Wilkinson JE,Shea T,DeLoughery C,Toukdarian A

更新日期：2002-01-01 00:00:00

abstract：:DNA replication initiation has been well-characterized; however, studies in the past few years have shown that there are still important discoveries to be made. Recent publications concerning the bacterial DnaA protein have revealed how this replication initiator, via interaction with specific sequences within the ori...

journal_title：Plasmid

authors： Zabrocka E,Wegrzyn K,Konieczny I

更新日期：2014-11-01 00:00:00

abstract：:The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...

journal_title：Plasmid

authors： Shammat IM,Welker DL

更新日期：1999-05-01 00:00:00

abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

journal_title：Plasmid

authors： Taylor DE,Brose EC

更新日期：1985-01-01 00:00:00

abstract：:A number of plasmid vectors were constructed for high-level gene expression in the dimorphic gram-negative bacterium Caulobacter crescentus. These vectors incorporate the transcription and translation initiation regions of the C. crescentus CB15A rsaA gene, which codes for the abundantly synthesized protein comprising...

journal_title：Plasmid

authors： Bingle WH,Smit J

更新日期：1990-09-01 00:00:00

abstract：:The incompatibility region of the Streptomyces plasmid pSL1 was identified as a 240-bp segment, though some other function from the vector plasmid was also necessary. A 540-bp fragment including the 240-bp region was enough for full expression of incompatibility. Inserted mutation analysis led to a more detailed locat...

journal_title：Plasmid

authors： Urabe H,Shindoh Y,Nakano MM,Ogawara H

更新日期：1987-03-01 00:00:00

abstract：:DNA sequence polymorphism was determined for the microcin V gene cluster encoded on the microcin V plasmids of 12 natural isolates of Escherichia coli. These microcin V gene clusters are similar in DNA sequence, with only 10 of the 683 bp polymorphic. Further, the levels and patterns of microcin V gene cluster polymor...

journal_title：Plasmid

authors： Pinou T,Riley MA

更新日期：2001-07-01 00:00:00

abstract：:In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there ...

journal_title：Plasmid

authors： Estruch F,Pérez-Ortín JE,Matallana E,Franco L

更新日期：1989-03-01 00:00:00

abstract：:Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of ...

journal_title：Plasmid

authors： Valverde V,Duplan H,Knibiehler M,Masson JM

更新日期：1994-07-01 00:00:00

abstract：:Recently the plasmid RK2 replication initiation protein, TrfA, has been shown to bind to the beta subunit of DNA Polymerase III (DnaN) via a short pentapeptide with the consensus QL[S/D]LF. A second consensus peptide, the hexapeptide QLxLxL, has also been demonstrated to mediate binding to DnaN. Here we describe the r...

journal_title：Plasmid

authors： Dalrymple BP,Kongsuwan K,Wijffels G

更新日期：2007-01-01 00:00:00

abstract：:Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techni...

journal_title：Plasmid

authors： Bao SH,Zhang DY,Meng E

更新日期：2019-09-01 00:00:00

abstract：:Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...

journal_title：Plasmid

authors： Mesas JM,Rodríguez MC,Alegre MT

更新日期：2001-09-01 00:00:00

abstract：:We have cloned and characterized an insertion sequence from Leuconostoc mesenteroides subsp. cremoris strain DB1165. This element, designated IS1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. The copy nu...

journal_title：Plasmid

authors： Johansen E,Kibenich A

更新日期：1992-05-01 00:00:00

abstract：:An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...

journal_title：Plasmid

authors： Hoang TT,Kutchma AJ,Becher A,Schweizer HP

更新日期：2000-01-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is require...

journal_title：Plasmid

authors： Goldbeck O,Seibold GM

更新日期：2018-01-01 00:00:00

abstract：:An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...

journal_title：Plasmid

authors： Bradshaw M,Goodnough MC,Johnson EA

更新日期：1998-11-01 00:00:00

abstract：:As part of a study identifying plasmids in Campylobacter, we isolated and sequenced two novel cryptic plasmids from an agricultural isolate of Campylobacter coli. The larger of the two plasmids, p3384, is 3316 bp in length and has a G+C content of 31.18%. A typical origin of replication consisting of five iterons was ...

journal_title：Plasmid

authors： Jesse TW,Pittenger-Alley LG,Englen MD

更新日期：2006-01-01 00:00:00

abstract：:Pseudomonas putida is a highly attractive production system for industrial needs. However, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a plasmid expressing a small RNA-based system with the potential to be used for di...

journal_title：Plasmid

authors： Apura P,Saramago M,Peregrina A,Viegas SC,Carvalho SM,Saraiva LM,Arraiano CM,Domingues S

更新日期：2020-05-01 00:00:00

abstract：:The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that...

journal_title：Plasmid

authors： Chakravartty V,Cronan JE

更新日期：2015-09-01 00:00:00

abstract：:Acinetobacter baumannii is an important nosocomial pathogen that often complicates treatment because of its high level of resistance to antibiotics. Though plasmids can potentially introduce various genes into bacterial strains, compared to other Gram-negative bacteria, information about the unique A. baumannii plasmi...

journal_title：Plasmid

authors： Hamidian M,Ambrose SJ,Hall RM

更新日期：2016-01-01 00:00:00

abstract：:Glycopeptide resistant Enterococcus faecium (GREF) persists on Norwegian poultry farms despite the ban on the growth promoter avoparcin. The biological basis for long-term persistence of avoparcin resistance is not fully understood. This study presents the complete DNA sequence of the E. faecium R-plasmid pVEF3 and fu...

journal_title：Plasmid

authors： Sletvold H,Johnsen PJ,Hamre I,Simonsen GS,Sundsfjord A,Nielsen KM

更新日期：2008-07-01 00:00:00