当前位置： SCI文献检索 > PLASMID期刊下所有文献 > Complete sequence of Enterococcus faecium pVEF3 and the detection of an omega-epsilon-zeta toxin-antitoxin module and an ABC transporter.

Complete sequence of Enterococcus faecium pVEF3 and the detection of an omega-epsilon-zeta toxin-antitoxin module and an ABC transporter.

Abstract：

:Glycopeptide resistant Enterococcus faecium (GREF) persists on Norwegian poultry farms despite the ban on the growth promoter avoparcin. The biological basis for long-term persistence of avoparcin resistance is not fully understood. This study presents the complete DNA sequence of the E. faecium R-plasmid pVEF3 and functional studies of some plasmid-encoded traits (a toxin-antitoxin (TA) system and an ABC transporter) that may be of importance for plasmid persistence. The pVEF3 (63.1 kbp), isolated from an E. faecium strain of poultry origin sampled in Norway in 1999, has 71 coding sequences including the vanA avoparcin/vancomycin resistance encoding gene cluster. pVEF3 encodes the TA system omega-epsilon-zeta, and plasmid stability tests and transcription analysis show that omega-epsilon-zeta is functional in Enterococcus faecalis OGIX, although with decreasing effect over time. The predicted ABC transporter was not found to confer reduced susceptibility to any of the 28 substances tested. The TA system identified in the pVEF-type plasmids may contribute to vanA plasmid persistence on Norwegian poultry farms. However, size and compositional heterogeneity among E. faecium vanA plasmids suggest that additional plasmid maintenance systems in combination with host specific factors and frequent horizontal gene transfer and rearrangement causes the observed plasmid composition and distribution patterns.

journal_name

Plasmid

journal_title

Plasmid

authors

Sletvold H,Johnsen PJ,Hamre I,Simonsen GS,Sundsfjord A,Nielsen KM

doi

10.1016/j.plasmid.2008.04.002

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

75-85

issue

1

eissn

0147-619X

issn

1095-9890

pii

S0147-619X(08)00040-1

journal_volume

60

pub_type

杂志文章

相关文献

PLASMID文献大全
  • A stable Escherichia coli-Mycobacterium smegmatis plasmid shuttle vector containing the mycobacteriophage D29 origin.

    abstract：:A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(92)90059-j

    authors： David M,Lubinsky-Mink S,Ben-Zvi A,Ulitzur S,Kuhn J,Suissa M

    更新日期：1992-11-01 00:00:00

  • Nucleotide sequence of the Pseudomonas aeruginosa insertion sequence IS222: another member of the IS3 family.

    abstract：:Sequence analysis of the Pseudomonas aeruginosa insertion sequence element IS222 revealed it to be 1234 bp in size with 23 bp imperfect terminal inverted repeats. Insertion caused a 5-bp duplication of the insertion site. Two ORFs were identified, one of which, ORFA, could encode a basic (pI 10.5) polypeptide with a m...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1994.1024

    authors： Kropinski AM,Farinha MA,Jansons I

    更新日期：1994-03-01 00:00:00

  • Maintenance of pBR322-derived plasmids without functional RNAI.

    abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(91)90042-u

    authors： Chiang CS,Bremer H

    更新日期：1991-11-01 00:00:00

  • Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58.

    abstract：:The construction of a set of new plasmids that are suitable as general cloning vectors in Escherichia coli and Agrobacterium tumefaciens is described. Plasmid pUCD2 is amplifiable in E. coli, replicates in a wide range of gram-negative hosts and contains a number of useful restriction endonuclear cleavage sites and an...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(84)90057-x

    authors： Close TJ,Zaitlin D,Kado CI

    更新日期：1984-09-01 00:00:00

  • The presence of repeated DNA sequences and a partial restriction map of the pSym of Rhizobium fredii USDA193.

    abstract：:The large, 350-kb Sym (symbiotic) plasmid pRjaUSDA193 of Rhizobium fredii was examined to determine the frequency of repeated sequences present and to produce a physical and genetic map of a large region of the plasmid. A novel hybridization method, the Southern Cross, revealed that the plasmid pRjaUSDA193 contained m...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(86)90077-6

    authors： Masterson RV,Atherly AG

    更新日期：1986-07-01 00:00:00

  • Efficient circularization in Escherichia coli of linear plasmid multimers from Dictyostelium discoideum genomic DNA.

    abstract：:Transformation of Escherichia coli with Dictyostelium discoideum genomic DNA containing integrated shuttle vectors in multicopy, tandemly duplicated format resulted in the establishment of the linear plasmid molecules as circular monomeric replicons. The transformation efficiencies were comparable to those obtained wi...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1996.0036

    authors： Barth C,Wilczynska Z,Pontes L,Fraser DJ,Fisher PR

    更新日期：1996-09-01 00:00:00

  • Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays.

    abstract：:Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2009.11.003

    authors： Miller CA 3rd,Tan X,Wilson M,Bhattacharyya S,Ludwig S

    更新日期：2010-03-01 00:00:00

  • An improved shuttle vector constructed for metabolic engineering research in Corynebacterium glutamicum.

    abstract：:Corynebacterium glutamicum is an industrial microorganism for production of amino acids. However, the metabolic engineering in C. glutamicum has been retarded due to lack of suitable vectors. In this study, we have constructed a shuttle vector pDXW-10 which harbors a large multiple cloning site suitable for cloning mu...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2010.05.004

    authors： Xu D,Tan Y,Shi F,Wang X

    更新日期：2010-09-01 00:00:00

  • The complete sequence and segregational stability analysis of a new cryptic plasmid pIGWZ12 from a clinical strain of Escherichia coli.

    abstract：:A new cryptic plasmid from a multi-resistant, multi-plasmid clinical strain of Escherichia coli has been isolated. The sequence of the 4072-base-pair pIGWZ12 (GenBank Accession No. DQ311641) was determined and analyzed. Two open-reading frames that code for proteins involved in plasmid mobilization and initiation of r...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2006.05.004

    authors： Zaleski P,Wolinowska R,Strzezek K,Lakomy A,Plucienniczak A

    更新日期：2006-11-01 00:00:00

  • A type Ib plasmid segregation machinery of the Advenella kashmirensis plasmid pBTK445.

    abstract：:pBTK445 is a newly described large (∼60Kb), low-copy number, conjugative plasmid indigenous to the sulfur-chemolithoautotroph Advenella kashmirensis. Based on its minimal replication region, a shuttle vector, pBTKS was constructed which can be used for diverse Alcaligenaceae members. The construct was found to be stab...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2010.12.007

    authors： Dam B

    更新日期：2011-03-01 00:00:00

  • Restriction map of virulence plasmid in Yersinia enterocolitica O:3.

    abstract：:Restriction map of the 72-kb virulence plasmid isolated from Yersinia enterocolitica O:3 was generated using EcoRI, BamHI, HindIII, and XbaI restriction enzymes. The mapping was done after cloning all of the 13 BamHI fragments of the plasmid in Escherichia coli. In addition, the restriction enzyme analysis revealed tw...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(86)90062-4

    authors： Pulkkinen L,Granberg I,Granfors K,Toivanen A

    更新日期：1986-11-01 00:00:00

  • Dosage of Tn916 circular intermediates in Enterococcus faecalis.

    abstract：:Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1995.1032

    authors： Manganelli R,Romano L,Ricci S,Zazzi M,Pozzi G

    更新日期：1995-07-01 00:00:00

  • Phylogenetic relationships among Vibrio anguillarum plasmids.

    abstract：:Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(84)90038-6

    authors： Mitoma Y,Aoki T,Crosa JH

    更新日期：1984-11-01 00:00:00

  • IS1473, a putative insertion sequence identified in the plasmid pAO1 from Arthrobacter nicotinovorans: isolation, characterization, and distribution among Arthrobacter species.

    abstract：:A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1996.1272

    authors： Menéndez C,Igloi GL,Brandsch R

    更新日期：1997-01-01 00:00:00

  • Plasmid pSM19035, a model to study stable maintenance in Firmicutes.

    abstract：:pSM19035 is a low-copy-number theta-replicating plasmid, which belongs to the Inc18 family. Plasmids of this family, which show a modular organization, are functional in evolutionarily diverse bacterial species of the Firmicutes Phylum. This review summarizes our understanding, accumulated during the last 20 years, on...

    journal_title：Plasmid

    pub_type： 杂志文章,评审

    doi：10.1016/j.plasmid.2010.04.002

    authors： Lioy VS,Pratto F,de la Hoz AB,Ayora S,Alonso JC

    更新日期：2010-07-01 00:00:00

  • Development of plasmid vector and electroporation condition for gene transfer in sporogenic lactic acid bacterium, Bacillus coagulans.

    abstract：:Bacillus coagulans is a sporogenic lactic acid bacterium that ferments glucose and xylose, major components of plant biomass, a potential feedstock for cellulosic ethanol. The temperature and pH for optimum rate of growth of B. coagulans (50 to 55 degrees C, pH 5.0) are very similar to that of commercially developed f...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2006.11.006

    authors： Rhee MS,Kim JW,Qian Y,Ingram LO,Shanmugam KT

    更新日期：2007-07-01 00:00:00

  • In vitro replication of cyanobacterial plasmids from Synechocystis PCC 6803.

    abstract：:Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1994.1055

    authors： Yang X,Daniell H,McFadden B

    更新日期：1994-09-01 00:00:00

  • Characterization of a novel plasmid-encoded HsdS subunit, S.LlaW12I, from Lactococcus lactis W12.

    abstract：:A novel type I restriction-modification specificity subunit, S. LlaW12I, has been identified on the naturally occurring 8.0-kb plasmid pAW122 in the lactic acid bacterium Lactococcus lactis subsp. cremoris W12. Presence of the HsdS protein together with a complete type I restriction-modification system conferred incre...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.2000.1478

    authors： Madsen A,Westphal C,Josephsen J

    更新日期：2000-09-01 00:00:00

  • Construction of a new polycistronic vector for over-expression and rapid purification of human hemoglobin.

    abstract：:To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2008.09.006

    authors： Domingues E,Brillet T,Vasseur C,Agier V,Marden MC,Baudin-Creuza V

    更新日期：2009-01-01 00:00:00

  • Search for a novel killer toxin in yeast Pichia pastoris.

    abstract：:Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1006/plas.1999.1452

    authors： Banerjee H,Verma M

    更新日期：2000-03-01 00:00:00

  • Development of a new pCAMBIA binary vector using Gateway® technology.

    abstract：:pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2015.07.003

    authors： Leclercq J,Szabolcs T,Martin F,Montoro P

    更新日期：2015-09-01 00:00:00

  • A runaway-replication plasmid pSY343 contains two ssi signals.

    abstract：:Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(88)90033-9

    authors： Bahk JD,Kioka N,Sakai H,Komano T

    更新日期：1988-11-01 00:00:00

  • An improved plasmid size standard, 39R861.

    abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2019.01.002

    authors： Moran RA,Anantham S,Hall RM

    更新日期：2019-03-01 00:00:00

  • New plasmid vector and host system for genetic engineering in Bacillus sphaericus.

    abstract：:A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(92)90026-7

    authors： Qiao M,Gao C,Cai B,Zhang X,Wang S

    更新日期：1992-05-01 00:00:00

  • Restriction endonuclease mapping of R27 (TP117), an incompatibility group HI subgroup 1 plasmid from Salmonella typhimurium.

    abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(85)90058-7

    authors： Taylor DE,Brose EC

    更新日期：1985-01-01 00:00:00

  • Characterization of pES213, a small mobilizable plasmid from Vibrio fischeri.

    abstract：:Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjug...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2005.01.003

    authors： Dunn AK,Martin MO,Stabb EV

    更新日期：2005-09-01 00:00:00

  • Convergent and overlapping transcripts of the Chlamydia trachomatis 7.5-kb plasmid.

    abstract：:Transcription of the 7.5-kb cryptic plasmid of Chlamydia trachomatis serovar L2 was investigated. Faint, diffuse transcripts of about 1.6 and 2.2 kb and intense short transcripts of about 250 and 430 bases were identified by Northern blot analysis. The short transcripts were found to have a common 5' end corresponding...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(92)90056-g

    authors： Fahr MJ,Sriprakash KS,Hatch TP

    更新日期：1992-11-01 00:00:00

  • Mutants of Enterococcus faecalis deficient as recipients in mating with donors carrying pheromone-inducible plasmids.

    abstract：:From Enterococcus faecalis cells containing random chromosomal insertions of Tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. The phage-resistant strains all showed decreased recipient ability (Con-) in broth matings with donors carrying pheromone-inducible plasmids. Thes...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(90)90025-8

    authors： Trotter KM,Dunny GM

    更新日期：1990-07-01 00:00:00

  • A physical and genetic map of the IncFI plasmid ColV2-K94.

    abstract：:A restriction enzyme map of the IncFI plasmid ColV2-K94 was generated using EcoRI, BamHI, HindIII, and XhoI; the genetic features of this element were then mapped from previous heteroduplex studies. ...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/0147-619x(85)90046-0

    authors： Weber PC,Palchaudhuri S

    更新日期：1985-05-01 00:00:00

  • Structural and sequence requirements for the antisense RNA regulating replication of staphylococcal multiresistance plasmid pSK41.

    abstract：:pSK41 is a prototypical 46-kb conjugative multiresistance plasmid of Staphylococcus aureus. The pSK41 replication initiation protein (Rep) is rate-limiting for plasmid replication, and its expression is negatively regulated by a small, non-coding antisense transcript, RNAI, that is complementary to the rep mRNA leader...

    journal_title：Plasmid

    pub_type： 杂志文章

    doi：10.1016/j.plasmid.2015.01.002

    authors： Kwong SM,Firth N

    更新日期：2015-03-01 00:00:00