Abstract:
:pSK41 is a prototypical 46-kb conjugative multiresistance plasmid of Staphylococcus aureus. The pSK41 replication initiation protein (Rep) is rate-limiting for plasmid replication, and its expression is negatively regulated by a small, non-coding antisense transcript, RNAI, that is complementary to the rep mRNA leader region. In this study, enzymatic probing was used to verify the predicted secondary structures of RNAI and its target RNA. We demonstrated that two stem-loop structures of RNAI, SLRNAI-II and SLRNAI-III, were important for inhibition. A putative U-turn motif detected in the loop of SLrep-I (5'-UUGG-3') was analysed for its significance to RNAI-mediated inhibition in vivo and Northern blotting suggested that rep mRNA was processed. Taken together, these observations support our previously proposed model but also raise new questions about the replication control mechanism.
journal_name
Plasmidjournal_title
Plasmidauthors
Kwong SM,Firth Ndoi
10.1016/j.plasmid.2015.01.002subject
Has Abstractpub_date
2015-03-01 00:00:00pages
17-25eissn
0147-619Xissn
1095-9890pii
S0147-619X(15)00013-Xjournal_volume
78pub_type
杂志文章相关文献
PLASMID文献大全abstract::A simple mathematical model of drug-induced plasmid elimination (curing) considering density-dependent growth rates and plasmid transfers is presented. It describes nonlinear population dynamics of conjugative plasmids during in vitro curing experiments in batch culture. The model was tested on kinetics of acridine or...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90052-8
更新日期:1989-03-01 00:00:00
abstract::A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/ Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0011
更新日期:1996-03-01 00:00:00
abstract::Unequal crossing-over is involved in genetic duplication and deletion in such diverse genetic systems as Drosophila, bacteria, and animal viruses. It is proposed to be involved in the form of unequal sister chromatid exchange in gene amplification in cultured animal cells and during carcinogenesis. Studies of the proc...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(85)90042-3
更新日期:1985-05-01 00:00:00
abstract::We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90069-0
更新日期:1983-09-01 00:00:00
abstract::We report the construction of two filamentous fungi Trichoderma reesei expression vectors, pWEF31 and pWEF32. Both vectors possess the hygromycin phosphotransferase B gene expression cassette and the strong promoter and terminator of the cellobiohydrolase 1 gene (cbh1) from T. reesei. The two newly constructed vectors...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2011.10.002
更新日期:2012-01-01 00:00:00
abstract::A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90026-7
更新日期:1992-05-01 00:00:00
abstract::We have constructed a pUC19-based multipurpose ATG vector in Schizosaccharomyces pombe with higher copy number and mitotic stability possible with commonly used vectors. The vector, having an NdeI site in its polylinker to provide ATG site for expression, carries a greatly truncated version of URA3 gene, URA3m, of Sac...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2012.07.001
更新日期:2012-11-01 00:00:00
abstract::The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is require...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2018.01.001
更新日期:2018-01-01 00:00:00
abstract::Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1467
更新日期:2000-05-01 00:00:00
abstract::Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a low-copy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization expe...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(85)90072-1
更新日期:1985-09-01 00:00:00
abstract::The incompatibility region of the Streptomyces plasmid pSL1 was identified as a 240-bp segment, though some other function from the vector plasmid was also necessary. A 540-bp fragment including the 240-bp region was enough for full expression of incompatibility. Inserted mutation analysis led to a more detailed locat...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90021-7
更新日期:1987-03-01 00:00:00
abstract::A simple and reliable method for the determination of plasmid copy numbers by direct fluorescence densitometry of ethidium bromide-stained electrophoretic gels was developed. In developing the method, the following parameters were evaluated and controlled: plasmid DNA trapping in the linear chromosomal DNA, staining-d...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90019-7
更新日期:1983-03-01 00:00:00
abstract::The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1008
更新日期:1995-01-01 00:00:00
abstract::Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integra...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1440
更新日期:2000-03-01 00:00:00
abstract::The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1045
更新日期:1993-09-01 00:00:00
abstract::Two distinct families of repetitive DNA elements (1.4 and 1.2 kb) were identified from S1 nuclease-treated genomic DNA of four strains of Thiobacillus ferrooxidans. The 1.4-kb fragment hybridized with IST2, an insertion sequence of T. ferrooxidans. The 1.2-kb fragment was cloned and sequenced. The sequence (IST445), 1...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1306
更新日期:1997-01-01 00:00:00
abstract::We report the mobilization by cointegration of the gonococcal 5.2 kb beta-lactamase plasmid pSJ5.2 in an Escherichia coli background. Transfer of pSJ5.2 was measured by filter mating assays with five different conjugative plasmids from Enterobacteriaceae and the gonococcal 41 kb tet(M). Plasmid pSJ5.2 was mobilized to...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.07.006
更新日期:2007-03-01 00:00:00
abstract::A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90059-j
更新日期:1992-11-01 00:00:00
abstract::The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90019-x
更新日期:1989-09-01 00:00:00
abstract::The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(91)90002-e
更新日期:1991-01-01 00:00:00
abstract::Bacillus coagulans is a sporogenic lactic acid bacterium that ferments glucose and xylose, major components of plant biomass, a potential feedstock for cellulosic ethanol. The temperature and pH for optimum rate of growth of B. coagulans (50 to 55 degrees C, pH 5.0) are very similar to that of commercially developed f...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.11.006
更新日期:2007-07-01 00:00:00
abstract::The chimeric plasmid pBN183 was first constructed in Escherichia coli by ligating the BamHI-digested E. coli plasmid pBR322 and a Bg/II-linearized streptococcal plasmid, pNZ18. The pBN183 transformed E. coli to ApR at a frequency of (8.2 +/- 1.2) x 10(5) colony forming units (CFU)/microgram DNA. Electrotransformation ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90033-7
更新日期:1992-07-01 00:00:00
abstract::We have evaluated the ability of immortalized human fibroblasts to recombine transfected plasmid DNA. A number of cell lines from normal individuals and from patients with DNA damage-processing defects were examined. Two plasmid recombination substrates were derived from pSV2neo and contained nonoverlapping deletions ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90030-9
更新日期:1989-07-01 00:00:00
abstract::pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.07.003
更新日期:2015-09-01 00:00:00
abstract::Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0042
更新日期:1996-11-01 00:00:00
abstract::Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90044-9
更新日期:1989-05-01 00:00:00
abstract::Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techni...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2019.102431
更新日期:2019-09-01 00:00:00
abstract::Two independently isolated temperature-sensitive bacteriophage that are specific for enterobacterial hosts harboring HI and HII plasmids were characterized to determine if any identifiable differences existed between them. The traits examined included adsorption pattern of phage to H pili, bacteriophage size, sensitiv...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(91)90055-2
更新日期:1991-09-01 00:00:00
abstract::Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90038-6
更新日期:1984-11-01 00:00:00
abstract::A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.1272
更新日期:1997-01-01 00:00:00