Abstract:
:A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This plasmid vector has been transformed into B. sphaericus AS 1.270, AS 1.465, AS 1.469, and 2362, at frequencies of 10(2)-10(3) transformants per microgram of DNA, and is maintained stably under nonselective conditions in these host strains. The presence of pNQ116 in B. sphaericus 2362 does ot interfere with the mosquito larvicidal activity of the organism.
journal_name
Plasmidjournal_title
Plasmidauthors
Qiao M,Gao C,Cai B,Zhang X,Wang Sdoi
10.1016/0147-619x(92)90026-7keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
237-41issue
3eissn
0147-619Xissn
1095-9890journal_volume
27pub_type
杂志文章相关文献
PLASMID文献大全abstract::A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restrictio...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1998.1380
更新日期:1999-01-01 00:00:00
abstract::We report herein the isolation and molecular characterization of pBGR1, the first native plasmid isolated from the genus Bartonella. Cloning and sequencing revealed a 2725-base pair (bp) cryptic plasmid comprising two open reading frames of considerable length, which were designated rep and mob. The regions containing...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00103-8
更新日期:2003-01-01 00:00:00
abstract::The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(87)90009-6
更新日期:1987-01-01 00:00:00
abstract::Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2005.08.004
更新日期:2006-03-01 00:00:00
abstract::Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1500
更新日期:2001-01-01 00:00:00
abstract::Transformation of Escherichia coli with Dictyostelium discoideum genomic DNA containing integrated shuttle vectors in multicopy, tandemly duplicated format resulted in the establishment of the linear plasmid molecules as circular monomeric replicons. The transformation efficiencies were comparable to those obtained wi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0036
更新日期:1996-09-01 00:00:00
abstract::The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1008
更新日期:1993-01-01 00:00:00
abstract::RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2005.11.008
更新日期:2006-05-01 00:00:00
abstract::Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2002.1565
更新日期:2002-03-01 00:00:00
abstract::Small particles of metallic tungsten, known also as tungsten microprojectiles, are routinely used for biotechnological purposes. In such applications, tungsten was observed to affect the integrity of plasmid DNA. Here we present evidence that interaction between tungsten particles and intact circular plasmids pU19, pU...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1475
更新日期:2000-07-01 00:00:00
abstract::A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimat...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1074
更新日期:1994-11-01 00:00:00
abstract::Temperate bacteriophage MAV1 is found in certain highly virulent strains of Mycoplasma arthritidis. Integration sites, portions of the right and left prophage ends, and flanking DNA from eight prophages in seven M. arthritidis strains were characterized in this study. attb and attp sites conformed for the most part to...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.04.004
更新日期:2004-07-01 00:00:00
abstract::Pseudomonas putida is a highly attractive production system for industrial needs. However, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a plasmid expressing a small RNA-based system with the potential to be used for di...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2020.102503
更新日期:2020-05-01 00:00:00
abstract::Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2001.1537
更新日期:2001-09-01 00:00:00
abstract::Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1412
更新日期:1999-09-01 00:00:00
abstract::Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1452
更新日期:2000-03-01 00:00:00
abstract::A new class of binary vectors has been constructed, containing the origin of replication of the W-type plasmid Sa. These vectors are designed to express foreign genes in plants under control of the TR gene 2' promoter or the promoter of a light-inducible ribulose-1,5-bisphosphate carboxylase small subunit gene of Arab...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90043-1
更新日期:1988-05-01 00:00:00
abstract::Corynebacterium glutamicum is an industrial microorganism for production of amino acids. However, the metabolic engineering in C. glutamicum has been retarded due to lack of suitable vectors. In this study, we have constructed a shuttle vector pDXW-10 which harbors a large multiple cloning site suitable for cloning mu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.05.004
更新日期:2010-09-01 00:00:00
abstract::To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirec...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2014.06.001
更新日期:2014-07-01 00:00:00
abstract::Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90044-9
更新日期:1989-05-01 00:00:00
abstract::We present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pKLH2 from an environmental bacterium, Acinetobacter calcoaceticus, isolated from a mercury mine. The pKLH2 mer operon has essentially the same gene organization as that of Tn21 and Tn501 from clinical bacteria. The pKLH...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1064
更新日期:1993-11-01 00:00:00
abstract::The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00100-2
更新日期:2002-09-01 00:00:00
abstract::A procedure for the isolation of closed circular mitochondrial DNA (mtDNA) from cells permeabilized with digitonin is described. Compared to the standard procedure in which cells are broken after osmotic swelling, the digitonin-based procedure is more consistent and results in higher mtDNA yields. ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(86)90083-1
更新日期:1986-07-01 00:00:00
abstract::A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2001.1550
更新日期:2002-01-01 00:00:00
abstract::We isolated a small multicopy cryptic plasmid, pNHK101, from Thermus sp. TK10 for use as a replicon of a Thermus expression vector. The nucleotide sequence of pNHK101 revealed that this plasmid was 1564bp long, with a total G+C content of 66.8%, which was in agreement with that of Thermus genomic DNA. The sequence did...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.12.004
更新日期:2005-07-01 00:00:00
abstract::pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.07.003
更新日期:2015-09-01 00:00:00
abstract::An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1998.1366
更新日期:1998-11-01 00:00:00
abstract::Some strains of the Enterobacteriaceae Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The genes responsible for this disease reside on a large, 155-kb plasmid designated amber disease-as...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(03)00062-3
更新日期:2003-11-01 00:00:00
abstract::The 4.2-kb cryptic plasmid pVA380-1 has been used as a vector for the cloning of antibiotic resistance genes directly in streptococci, and in the construction of Escherichia coli/Streptococcus shuttle vectors. The results of subcloning experiments located the basic replicon of pVA380-1 within a 2.5-kb region. The nucl...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90044-b
更新日期:1992-09-01 00:00:00
abstract::Bacillus mycoides, a member of the Bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. Two plasmids, one from a strain curving to the right (pDx14.2),...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2005.05.002
更新日期:2005-11-01 00:00:00