IS1473, a putative insertion sequence identified in the plasmid pAO1 from Arthrobacter nicotinovorans: isolation, characterization, and distribution among Arthrobacter species.

abstract：:Corynebacterium glutamicum is an industrial microorganism for production of amino acids. However, the metabolic engineering in C. glutamicum has been retarded due to lack of suitable vectors. In this study, we have constructed a shuttle vector pDXW-10 which harbors a large multiple cloning site suitable for cloning mu...

journal_title：Plasmid

authors： Xu D,Tan Y,Shi F,Wang X

更新日期：2010-09-01 00:00:00

abstract：:Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....

journal_title：Plasmid

authors： Dumans AT,Carvalho MF,Coelho A

更新日期：1989-11-01 00:00:00

abstract：:By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sens...

journal_title：Plasmid

authors： Maas R

更新日期：1983-11-01 00:00:00

abstract：:Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...

journal_title：Plasmid

authors： Peters M,Tomikas A,Nurk A

更新日期：2004-11-01 00:00:00

abstract：:We report the characterization of Tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the R-plasmid pGD10. Tn602 is highly homologous to the previously characterized Tn903, present on the R-plasmid R6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-I (hom...

journal_title：Plasmid

authors： Stibitz S,Davies JE

更新日期：1987-05-01 00:00:00

abstract：:A combined physical and functional map of plasmid RP4 is presented including the sites for 18 restriction endonucleases. Several cleavage sites of SphI, BalI, and ApaI are suitable for the dissection of the transfer gene regions. Recombinant plasmids containing RP4 SphI fragments were constructed to assist in localizi...

journal_title：Plasmid

authors： Lanka E,Lurz R,Fürste JP

更新日期：1983-11-01 00:00:00

abstract：:A transient expression system has been developed to compare the relative efficiency of expression of various vaccinia virus DNA sequences containing transcriptional regulatory elements. A plasmid vector was constructed containing both the Escherichia coli galactokinase gene (galK) and the guanine phosphoribosyltransfe...

journal_title：Plasmid

authors： Shepard B,Panicali D,Huang C

更新日期：1987-07-01 00:00:00

abstract：:Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...

journal_title：Plasmid

authors： Mesas JM,Rodríguez MC,Alegre MT

更新日期：2001-09-01 00:00:00

abstract：:pIP501 and pAM beta 1 are promiscuous plasmids from gram-positive bacteria which carry structurally and genetically related replication functions (L. Jannière, A. Gruss and S.D. Ehrlich, 1993, in "Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (A.L. Sonenshein, J.A...

journal_title：Plasmid

authors： Pujol C,Ehrlich SD,Jannière L

更新日期：1994-01-01 00:00:00

abstract：:The sequences of three transfer RNAs from mosquito cell mitochondria, tRNAArgUCG, tRNAAspGUC, and tRNAIleGAU, determined using a combination of rapid ladder and fingerprinting procedures are reported. These were compared with hamster mitochondrial tRNAArgUCG and tRNAAspGUC determined similarly, and a bovine mitochondr...

journal_title：Plasmid

authors： HsuChen CC,Cleaves GR,Dubin DT

更新日期：1983-07-01 00:00:00

abstract：:The structural and functional properties of mercury resistance determinants cloned from a series of independently isolated conjugative plasmids were compared with those of the prototype HgR determinants from Tn501 and plasmid R100 (containing Tn21). Restriction endonuclease mapping classified the HgR determinants into...

journal_title：Plasmid

authors： Jobling MG,Peters SE,Ritchie DA

更新日期：1988-09-01 00:00:00

abstract：:The ability of some bacteria to grow in the presence of high concentrations of tellurium compounds has been recognized for almost 100 years. Since then, interest in this phenomenon has generated a slow but steady trickle of literature. In the past few years, the use of modern techniques in molecular biology has led to...

journal_title：Plasmid

authors： Walter EG,Taylor DE

更新日期：1992-01-01 00:00:00

abstract：:Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and...

journal_title：Plasmid

authors： Wehnert GU,Abratt VR,Goodman HJ,Woods DR

更新日期：1990-03-01 00:00:00

abstract：:Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...

journal_title：Plasmid

authors： Caufield PW,Shah G,Hollingshead SK,Parrot M,Lavoie MC

更新日期：1990-09-01 00:00:00

abstract：:A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective mediu...

journal_title：Plasmid

authors： Xia XS,Aathithan S,Oswiecimska K,Smith AR,Bruce IJ

更新日期：1998-01-01 00:00:00

abstract：:Even though Vibrio cholerae is a well-known human pathogen, it is also a normal member of aquatic habitats. Within this environment it often forms biofilms on the chitin-containing exoskeleton of crustaceans and their molts. Chitin not only serves as nutrient source but also induces a developmental program called natu...

journal_title：Plasmid

authors： De Souza Silva O,Blokesch M

更新日期：2010-11-01 00:00:00

abstract：:Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...

journal_title：Plasmid

authors： Feng X,Ou LT,Ogram A

更新日期：1997-01-01 00:00:00

abstract：:We present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pKLH2 from an environmental bacterium, Acinetobacter calcoaceticus, isolated from a mercury mine. The pKLH2 mer operon has essentially the same gene organization as that of Tn21 and Tn501 from clinical bacteria. The pKLH...

journal_title：Plasmid

authors： Kholodii GY,Gorlenko Z,Lomovskaya OL,Mindlin SZ,Yurieva OV,Nikiforov VG

更新日期：1993-11-01 00:00:00

abstract：:CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...

journal_title：Plasmid

authors： Kang N,Choi E,Kim SG,Hwang J

更新日期：2015-09-01 00:00:00

abstract：:The cloning of the finO region from the antibiotic-resistance plasmid R6-5 is reported. On the basis of DNA deletion analysis and Tn5 transposon insertional mutagenesis of finO+ chimeric plasmids, finO has been located within the coordinates 94.0-94.85 on the R6-5 map. A 32,000-Da polypeptide (32K), which is encoded w...

journal_title：Plasmid

authors： Cheah KC,Ray A,Skurray R

更新日期：1984-11-01 00:00:00

abstract：:Temperate bacteriophage MAV1 is found in certain highly virulent strains of Mycoplasma arthritidis. Integration sites, portions of the right and left prophage ends, and flanking DNA from eight prophages in seven M. arthritidis strains were characterized in this study. attb and attp sites conformed for the most part to...

journal_title：Plasmid

authors： Washburn LR,Miller EJ,Mukherjee S,Dannenbring D

更新日期：2004-07-01 00:00:00

abstract：:Functional analysis of cloned genes often makes use of complementation after introducing these genes into cells of a mutant strain. Problems with this self-cloning step in the cyanobacterium Anacystis nidulans R2 have been encountered, which were mainly due to recombinational instability of gene and vector after trans...

journal_title：Plasmid

authors： Kuhlemeier CJ,Hardon EM,van Arkel GA,van de Vate C

更新日期：1985-11-01 00:00:00

abstract：:We isolated a small multicopy cryptic plasmid, pNHK101, from Thermus sp. TK10 for use as a replicon of a Thermus expression vector. The nucleotide sequence of pNHK101 revealed that this plasmid was 1564bp long, with a total G+C content of 66.8%, which was in agreement with that of Thermus genomic DNA. The sequence did...

journal_title：Plasmid

authors： Kobayashi H,Kuwae A,Maseda H,Nakamura A,Hoshino T

更新日期：2005-07-01 00:00:00

abstract：:Bacterial chromosomes segregate via a partition apparatus that employs a score of specialized proteins. The SMC complexes play a crucial role in the chromosome partitioning process by organizing bacterial chromosomes through their ATP-dependent chromatin-compacting activity. Recent progress in the composition of these...

journal_title：Plasmid

authors： Strunnikov AV

更新日期：2006-03-01 00:00:00

abstract：:We report herein the isolation and molecular characterization of pBGR1, the first native plasmid isolated from the genus Bartonella. Cloning and sequencing revealed a 2725-base pair (bp) cryptic plasmid comprising two open reading frames of considerable length, which were designated rep and mob. The regions containing...

journal_title：Plasmid

authors： Seubert A,Falch C,Birtles RJ,Schulein R,Dehio C

更新日期：2003-01-01 00:00:00

abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

journal_title：Plasmid

authors： Taylor DE,Brose EC

更新日期：1985-01-01 00:00:00

abstract：:RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...

journal_title：Plasmid

authors： Sotirova VN,Calciano MA,Krueger W,Lalande M

更新日期：2006-05-01 00:00:00

abstract：:The stable maintenance of certain plasmids in bacterial populations has contributed significantly to the current worldwide antibiotic resistance (AbR) emergency. IncX plasmids, long underestimated in this regard, have achieved recent notoriety for their roles in transmission of resistance to carbapenem and colistin, t...

journal_title：Plasmid

authors： Bustamante P,Iredell JR

更新日期：2017-05-01 00:00:00

abstract：:The expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in heart allografts is an important mechanism in response to ischemia/reperfusion (I/R) injury which represents the single major non-immunologic factor implicated in pathogenesis of chronic graft dysfunction (CGD). Adenoviral mediated overexpression of HIF...

journal_title：Plasmid

authors： Wei L,Lu J,Feng L,Li S,Shan J,Li Y

更新日期：2010-01-01 00:00:00

abstract：:The gene(s) for chromosome-mediated beta-lactamase production of Proteus vulgaris GN7919 was cloned into a unique EcoRI site of pACYC184 as an insert of a 14.2-kb fragment, which was further digested into two fragments with EcoRI, 4.9 and 9.3 kb. The restriction enzyme digestion pattern of the recombinant plasmid, des...

journal_title：Plasmid

authors： Maejima T,Ohya Y,Mitsuhashi S,Inoue M

更新日期：1987-09-01 00:00:00