Isolation and characterization of an extrachromosomal element from Nocardia mediterranei.

Abstract:

:Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a low-copy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization experiments showed that pMEA 100 is present in strain LBG A3136 in the free as well as in the integrated form whereas in strain ATCC 13685 only an integrated form was detected. Excision and reintegration in strain LBG A3136 seemed to be site specific. pMEA100 was found to be self-transmissible, eliciting the lethal zygosis phenotype, and is possibly involved in fertility in N. mediterranei.

journal_name

Plasmid

journal_title

Plasmid

authors

Moretti P,Hintermann G,Hütter R

doi

10.1016/0147-619x(85)90072-1

subject

Has Abstract

pub_date

1985-09-01 00:00:00

pages

126-33

issue

2

eissn

0147-619X

issn

1095-9890

pii

0147-619X(85)90072-1

journal_volume

14

pub_type

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