Abstract:
:A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb and can be introduced via transformation into Mycobacterium smegmatis with high efficiency. In M. smegmatis the plasmid is stable and apparently present in multiple copies. Bioluminescence (luxA and luxB of Vibrio harveyi and fischeri) has been expressed in M. smegmatis from the aminoglycoside transferase promoter of Tn5. The D29 fragment should carry an origin of replication and some associated genes that act on it since various mutations destroy the ability of this fragment to replicate in M. smegmatis. The fragment was localized on the D29 genome map.
journal_name
Plasmidjournal_title
Plasmidauthors
David M,Lubinsky-Mink S,Ben-Zvi A,Ulitzur S,Kuhn J,Suissa Mdoi
10.1016/0147-619x(92)90059-jkeywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
267-71issue
3eissn
0147-619Xissn
1095-9890journal_volume
28pub_type
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