Abstract:
:Identification of the Sym plasmid in Rhizobium phaseoli strain RCC3622 is described. Introduction of this plasmid into R. trifolii or Agrobacterium tumefaciens strains resulted in bacteria capable of forming characteristic spherical root nodules on beans. This Sym plasmid, designated pSym9, was characterized as 275 MDa and nonconjugative. pSym9 was incompatible with the R. trifolii Sym plasmid pSym5, and carries genes determining a melanin-like black pigment. A second plasmid of 135 MDa, pRph3622a, was also transferred from R. phaseoli to R. trifolii and A. tumefaciens. Transconjugants carrying this plasmid did not form root nodules on beans. In contrast to other Rhizobium plasmids, pRph3622a was unstable in A. tumefaciens.
journal_name
Plasmidjournal_title
Plasmidauthors
Hooykaas PJ,den Dulk-Ras H,Regensburg-Tuïnk AJ,van Brussel AA,Schilperoort RAdoi
10.1016/0147-619x(85)90031-9subject
Has Abstractpub_date
1985-07-01 00:00:00pages
47-52issue
1eissn
0147-619Xissn
1095-9890pii
0147-619X(85)90031-9journal_volume
14pub_type
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