Abstract:
:The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number plasmid (pFD 383) encodes cytochrome P450 C14 alpha lanosterol demethylase under the control of phospho-glycerate-kinase promoter. S. cerevisiae transformed by the pFD 383 plasmid are resistant to imidazoles and triazoles. Moreover, this transformed strain shows increased levels of P45014DM mRNA and of cytochrome P450. A second low copy number plasmid (pFD 384) carries a mutant cytochrome P450 14 alpha lanosterol demethylase gene, which increases imidazole and triazole resistance. These constructions can be used on a dominant selection marker to transform wild-type yeasts and to confer imidazole and triazole resistance in industrial fermentation.
journal_name
Plasmidjournal_title
Plasmidauthors
Doignon F,Aigle M,Ribereau-Gayon Pdoi
10.1006/plas.1993.1054subject
Has Abstractpub_date
1993-11-01 00:00:00pages
224-33issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(83)71054-1journal_volume
30pub_type
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