Abstract:
:Nocardia brasiliensis, is a bacteria that lives as saprophyte in soil and causes a disease called actinomycetoma in both human and animals. Nocardia brasiliensis is an intracellular, facultative bacterium that replicates and survives within host macrophages. The mechanisms involved in the evasion of the microbicidal actions of macrophages remain unclear. The filamentous growth of N. brasiliensis is resistant to unicellular preparations, leading to inaccurate quantification of bacterial numbers by means of colony forming units (CFU). As successful survival studies with green fluorescent protein (GFP)-expressing bacterial strains have been reported, we constructed a recombinant GFP-expressing strain of N. brasiliensis. The virulence of the modified strain is maintained because it induces mycetoma in BALB/c mice. This new strain can be used for bacterial survival assays using cytometry and to elucidate the pathogenicity mechanisms in Actinomycetoma infection.
journal_name
Plasmidjournal_title
Plasmidauthors
Salinas-Carmona MC,Rocha-Pizaña MRdoi
10.1016/j.plasmid.2010.09.005subject
Has Abstractpub_date
2011-01-01 00:00:00pages
25-31issue
1eissn
0147-619Xissn
1095-9890pii
S0147-619X(10)00089-2journal_volume
65pub_type
杂志文章相关文献
PLASMID文献大全abstract::Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1029
更新日期:1994-05-01 00:00:00
abstract::Two distinct families of repetitive DNA elements (1.4 and 1.2 kb) were identified from S1 nuclease-treated genomic DNA of four strains of Thiobacillus ferrooxidans. The 1.4-kb fragment hybridized with IST2, an insertion sequence of T. ferrooxidans. The 1.2-kb fragment was cloned and sequenced. The sequence (IST445), 1...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1306
更新日期:1997-01-01 00:00:00
abstract::Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90033-9
更新日期:1988-11-01 00:00:00
abstract::As multidrug resistant bacteria pose one of the greatest risks to human health new alternative antibacterial agents are urgently needed. One possible mechanism that can be used as an alternative to traditional antibiotic therapy is transfer of killing agents via conjugation. Our work was aimed at providing a proof of ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.10.001
更新日期:2015-11-01 00:00:00
abstract::A preliminary screening study of six Moraxella catarrhalis isolates from primary school children in the Netherlands identified a small 3.5 kb plasmid (pEMCJH03), containing four open reading frames, which encoded three mobilizing and one replicase protein. Insertion of a kanamycin containing transposon (yielding pEMCJ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2004.11.003
更新日期:2005-05-01 00:00:00
abstract::Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a low-copy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization expe...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(85)90072-1
更新日期:1985-09-01 00:00:00
abstract::During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1062
更新日期:1994-09-01 00:00:00
abstract::To study the transcriptional activity of the HIV-1 LTR, we constructed a vector containing Renilla and Firefly luciferase genes under the control of the LTR (wild-type or mutated version) and oriented in a manner that allowed them to be transcribed in opposite directions. We found that the HIV-1 LTR acted as a bidirec...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2014.06.001
更新日期:2014-07-01 00:00:00
abstract::A chimeric plasmid, pRS7Rep (6.1 kb), was constructed using the replication region of pRS7, a large plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli using its pIJ2925 componen...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2014.11.004
更新日期:2015-01-01 00:00:00
abstract::The replication (rep) regions of small plasmids from three Sinorhizobium meliloti strains were cloned by marker rescue. Two unique replication regions were identified, one of which was common to two different strains. Plasmid pBB83 carried a 7.2 kbp rep region from a 42 kbp plasmid, and pBB84 carried a 4.5 kbp rep reg...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2005.08.003
更新日期:2006-03-01 00:00:00
abstract::A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90059-j
更新日期:1992-11-01 00:00:00
abstract::It is now known that conjugative pili are determined by representative plasmids for all incompatibility groups in Escherichia coli K-12. They fall into three basic morphological groups, which are described: thin flexible, thick flexible, and rigid filaments or rods. The main thrust of this study, however, has been the...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(80)90005-0
更新日期:1980-09-01 00:00:00
abstract::Identification of the Sym plasmid in Rhizobium phaseoli strain RCC3622 is described. Introduction of this plasmid into R. trifolii or Agrobacterium tumefaciens strains resulted in bacteria capable of forming characteristic spherical root nodules on beans. This Sym plasmid, designated pSym9, was characterized as 275 MD...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(85)90031-9
更新日期:1985-07-01 00:00:00
abstract::This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2004.12.008
更新日期:2005-03-01 00:00:00
abstract::The fission yeast Schizosaccharomyces pombe is an attractive host for heterologous gene expression. However, expression systems for industrially viable large-scale fermentations are scarce. Several inducible expression vectors for S. pombe have been reported, with the strong thiamine-repressible nmt1+ promoter or deri...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2017.11.002
更新日期:2018-01-01 00:00:00
abstract::A suppressor of the mutations which inhibit the establishment of lysogeny in Pseudomonas aeruginosa has been found to be encoded by P. aeruginosa plasmids of four separate incompatibility groups. The wide distribution of this phenotype (designated Sly for suppressor of lysogeny establishment deficiency) indicates that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90024-6
更新日期:1984-03-01 00:00:00
abstract::The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1008
更新日期:1995-01-01 00:00:00
abstract::The chimeric plasmid pBN183 was first constructed in Escherichia coli by ligating the BamHI-digested E. coli plasmid pBR322 and a Bg/II-linearized streptococcal plasmid, pNZ18. The pBN183 transformed E. coli to ApR at a frequency of (8.2 +/- 1.2) x 10(5) colony forming units (CFU)/microgram DNA. Electrotransformation ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(92)90033-7
更新日期:1992-07-01 00:00:00
abstract::The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00100-2
更新日期:2002-09-01 00:00:00
abstract::Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1994.1041
更新日期:1994-07-01 00:00:00
abstract::Expression vectors for industrial production should be stable and allow tight control of protein synthesis. This is necessary to ensure plasmid transmission to daughter cells in order to achieve a stable population capable of synthesizing high amounts of the target protein. A high-copy-number plasmid, pAE, was previou...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2018.08.004
更新日期:2018-06-01 00:00:00
abstract::Variovorax sp. strain DB1 and Pseudomonas pickettii strain 712 are 2,4-dicholorophenoxy-acetic acid (2,4-D)-degrading bacteria, which were isolated from agricultural soils in Republic of Korea and USA, respectively. Each strain harbors a 2,4-D degradative plasmid and is able to utilize 2,4-D as the sole source of carb...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2013.01.003
更新日期:2013-05-01 00:00:00
abstract::In the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pD2RT of Pseudomonas migulae strain D2RT isolated from Baltic Sea water. The pD2RT is 129,894 base pairs in size with an average G+C content of 53.75%. A total of 135 open reading frames (ORFs) were predicted to encode pro...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2013.09.003
更新日期:2013-11-01 00:00:00
abstract::A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy Australian adults were screened using PCR-based replicon typing. Each isolate represented a distinct strain found in a particular faecal sample. Fifty-one isolates were resistant to one or more of 12 antibiotics tested. FII and...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.03.005
更新日期:2015-07-01 00:00:00
abstract::Recently the plasmid RK2 replication initiation protein, TrfA, has been shown to bind to the beta subunit of DNA Polymerase III (DnaN) via a short pentapeptide with the consensus QL[S/D]LF. A second consensus peptide, the hexapeptide QLxLxL, has also been demonstrated to mediate binding to DnaN. Here we describe the r...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.07.005
更新日期:2007-01-01 00:00:00
abstract::Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1452
更新日期:2000-03-01 00:00:00
abstract:UNLABELLED:This work describes a novel plasmid pSP187 (5550 bp) carrying the small multidrug resistance determinant smr encoding resistance to quaternary ammonium compounds (QACs). pSP187 was identified in a Staphylococcus pasteuri isolate recovered from bulk milk in a dairy cattle herd in Norway. Sequence analysis rev...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2006.08.004
更新日期:2007-03-01 00:00:00
abstract::A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/ Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0011
更新日期:1996-03-01 00:00:00
abstract::Previous explanations of why bacterial genes for certain "optional" traits tend to occur on plasmids rather than chromosomes are based on an outdated misunderstanding of natural selection. They also fail to explain why certain characters that are ubiquitous in some bacterial species tend to occur on plasmids. This pap...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/0147-619x(89)90040-1
更新日期:1989-05-01 00:00:00
abstract::A combined physical and functional map of plasmid RP4 is presented including the sites for 18 restriction endonucleases. Several cleavage sites of SphI, BalI, and ApaI are suitable for the dissection of the transfer gene regions. Recombinant plasmids containing RP4 SphI fragments were constructed to assist in localizi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(83)90047-1
更新日期:1983-11-01 00:00:00