Characterization of the cryptic plasmid pCC1 from Corynebacterium callunae and its use for vector construction.

abstract：:A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...

journal_title：Plasmid

authors： Roy RN,Bigelow N,Dillon JA

更新日期：1988-01-01 00:00:00

abstract：:Mutations that increase the copy number of the pSC101 replicon have been used for construction of new cloning vectors. Replacement of glutamate at position 93 in RepA yields plasmids that replicate at medium (27 copies/cell) and high (approximately 240 copies/cell) copy numbers. Based on the crystal structure of RepE,...

journal_title：Plasmid

authors： Peterson J,Phillips GJ

更新日期：2008-05-01 00:00:00

abstract：:Mobile plasmid-encoded elements are DNA segments that are transferred for horizontal gene transfer and that confer adaptive proprieties, as well as virulence and antibiotic and heavy metal resistance to bacteria. The conjugative plasmid pUM505, isolated from a clinical strain of Pseudomonas aeruginosa, possesses a put...

journal_title：Plasmid

authors： Hernández-Ramírez KC,Reyes-Gallegos RI,Chávez-Jacobo VM,Díaz-Magaña A,Meza-Carmen V,Ramírez-Díaz MI

更新日期：2018-06-01 00:00:00

abstract：:RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...

journal_title：Plasmid

authors： Sotirova VN,Calciano MA,Krueger W,Lalande M

更新日期：2006-05-01 00:00:00

abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...

journal_title：Plasmid

authors： Yamamoto T,Hanawa T,Murayama SY,Ogata S

更新日期：1994-09-01 00:00:00

abstract：:A transient expression system has been developed to compare the relative efficiency of expression of various vaccinia virus DNA sequences containing transcriptional regulatory elements. A plasmid vector was constructed containing both the Escherichia coli galactokinase gene (galK) and the guanine phosphoribosyltransfe...

journal_title：Plasmid

authors： Shepard B,Panicali D,Huang C

更新日期：1987-07-01 00:00:00

abstract：:We describe the construction of a series of shuttle vectors for Staphylococcus aureus. In order to determine transcriptional regulation by essential regulators, we constructed promoterless luxABCDE reporter system using a TetR-regulated antisense RNA expression vector, pJYJ909, which is composed of S. aureus plasmid p...

journal_title：Plasmid

authors： Yan M,Yu C,Yang J,Ji Y

更新日期：2009-05-01 00:00:00

abstract：:Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techni...

journal_title：Plasmid

authors： Bao SH,Zhang DY,Meng E

更新日期：2019-09-01 00:00:00

abstract：:Recently the plasmid RK2 replication initiation protein, TrfA, has been shown to bind to the beta subunit of DNA Polymerase III (DnaN) via a short pentapeptide with the consensus QL[S/D]LF. A second consensus peptide, the hexapeptide QLxLxL, has also been demonstrated to mediate binding to DnaN. Here we describe the r...

journal_title：Plasmid

authors： Dalrymple BP,Kongsuwan K,Wijffels G

更新日期：2007-01-01 00:00:00

abstract：:Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...

journal_title：Plasmid

authors： Allignet J,El Solh N

更新日期：1999-09-01 00:00:00

abstract：:An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...

journal_title：Plasmid

authors： Bradshaw M,Goodnough MC,Johnson EA

更新日期：1998-11-01 00:00:00

abstract：:pSK41 is a prototypical 46-kb conjugative multiresistance plasmid of Staphylococcus aureus. The pSK41 replication initiation protein (Rep) is rate-limiting for plasmid replication, and its expression is negatively regulated by a small, non-coding antisense transcript, RNAI, that is complementary to the rep mRNA leader...

journal_title：Plasmid

authors： Kwong SM,Firth N

更新日期：2015-03-01 00:00:00

abstract：:Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...

journal_title：Plasmid

authors： Yang X,Daniell H,McFadden B

更新日期：1994-09-01 00:00:00

abstract：:Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...

journal_title：Plasmid

authors： Boe L,Rasmussen KV

更新日期：1996-11-01 00:00:00

abstract：:Glycopeptide resistant Enterococcus faecium (GREF) persists on Norwegian poultry farms despite the ban on the growth promoter avoparcin. The biological basis for long-term persistence of avoparcin resistance is not fully understood. This study presents the complete DNA sequence of the E. faecium R-plasmid pVEF3 and fu...

journal_title：Plasmid

authors： Sletvold H,Johnsen PJ,Hamre I,Simonsen GS,Sundsfjord A,Nielsen KM

更新日期：2008-07-01 00:00:00

abstract：:A suppressor of the mutations which inhibit the establishment of lysogeny in Pseudomonas aeruginosa has been found to be encoded by P. aeruginosa plasmids of four separate incompatibility groups. The wide distribution of this phenotype (designated Sly for suppressor of lysogeny establishment deficiency) indicates that...

journal_title：Plasmid

authors： Mondello FJ,Miller RV

更新日期：1984-03-01 00:00:00

abstract：:A combined physical and functional map of plasmid RP4 is presented including the sites for 18 restriction endonucleases. Several cleavage sites of SphI, BalI, and ApaI are suitable for the dissection of the transfer gene regions. Recombinant plasmids containing RP4 SphI fragments were constructed to assist in localizi...

journal_title：Plasmid

authors： Lanka E,Lurz R,Fürste JP

更新日期：1983-11-01 00:00:00

abstract：:Aeromonas hydrophila strain AO1 isolated from an infected fish was found to be resistant to several quinolones. A plasmid isolated from the strain AO1, termed pBRST7.6, was cloned and sequenced and shown to be 7621bp in length with a GC content of 60%. Further analysis confirmed that it contained a gene with 100% iden...

journal_title：Plasmid

authors： Majumdar T,Das B,Bhadra RK,Dam B,Mazumder S

更新日期：2011-07-01 00:00:00

abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

journal_title：Plasmid

authors： Chiang CS,Bremer H

更新日期：1991-11-01 00:00:00

abstract：:Fusobacterium nucleatum is a Gram-negative anaerobic rod found in dental plaque biofilms, and is an opportunistic pathogen implicated in periodontitis as well as a wide range of systemic abscesses and infections. Genomic analyses of F. nucleatum indicate considerable genetic diversity and a prominent role for horizont...

journal_title：Plasmid

authors： Claypool BM,Yoder SC,Citron DM,Finegold SM,Goldstein EJ,Haake SK

更新日期：2010-01-01 00:00:00

abstract：:Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging fro...

journal_title：Plasmid

authors： Fornari CS,Watkins M,Kaplan S

更新日期：1984-01-01 00:00:00

abstract：:We report herein the isolation and molecular characterization of pBGR1, the first native plasmid isolated from the genus Bartonella. Cloning and sequencing revealed a 2725-base pair (bp) cryptic plasmid comprising two open reading frames of considerable length, which were designated rep and mob. The regions containing...

journal_title：Plasmid

authors： Seubert A,Falch C,Birtles RJ,Schulein R,Dehio C

更新日期：2003-01-01 00:00:00

abstract：:The four plasmids in E. coli 39R861 have been used as size standards for closed-circular plasmid DNA since the 1980s. However, their sizes had only been estimated. Here, the complete sequence of the 61.3 kb cryptic FII plasmid p39R861-3 is reported, and the sizes of p39R861-4, pSa and NTP168 were determined to be 155....

journal_title：Plasmid

authors： Moran RA,Anantham S,Hall RM

更新日期：2019-03-01 00:00:00

abstract：:The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative ...

journal_title：Plasmid

authors： Zheng H,Chang XB,Wilson JH

更新日期：1989-09-01 00:00:00

abstract：:pCAMBIA vectors have become popular for their easy handling, stability and the existence of a range of selection and reporter genes. However, these vectors have yet to integrate the Gateway® cloning system, which has enabled site-specific recombination without the need for restriction enzymes and ligases. This paper s...

journal_title：Plasmid

authors： Leclercq J,Szabolcs T,Martin F,Montoro P

更新日期：2015-09-01 00:00:00

abstract：:We report here the molecular characterization of pFNL10, a 3990-bp cryptic plasmid of Francisella novicida-like F6168. The plasmid was maintained in F. novicida Utah 112 and F. tularensis LVS strains. We sequenced the entire plasmid and found six open reading frames (ORFs)-ORF1, ORF2, ORF3, ORF4, ORF5, and ORFm. ORF3,...

journal_title：Plasmid

authors： Pomerantsev AP,Golovliov IR,Ohara Y,Mokrievich AN,Obuchi M,Norqvist A,Kuoppa K,Pavlov VM

更新日期：2001-11-01 00:00:00

abstract：:Functional analysis of cloned genes often makes use of complementation after introducing these genes into cells of a mutant strain. Problems with this self-cloning step in the cyanobacterium Anacystis nidulans R2 have been encountered, which were mainly due to recombinational instability of gene and vector after trans...

journal_title：Plasmid

authors： Kuhlemeier CJ,Hardon EM,van Arkel GA,van de Vate C

更新日期：1985-11-01 00:00:00

abstract：:Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...

journal_title：Plasmid

authors： Banerjee H,Verma M

更新日期：2000-03-01 00:00:00

abstract：:The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826...

journal_title：Plasmid

authors： Dalal J,Yalamanchili R,La Hovary C,Ji M,Rodriguez-Welsh M,Aslett D,Ganapathy S,Grunden A,Sederoff H,Qu R

更新日期：2015-09-01 00:00:00

abstract：:The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...

journal_title：Plasmid

authors： Osbourn SE,Turner AK,Grinsted J

更新日期：1995-01-01 00:00:00