Abstract:
:Bacillus coagulans is a sporogenic lactic acid bacterium that ferments glucose and xylose, major components of plant biomass, a potential feedstock for cellulosic ethanol. The temperature and pH for optimum rate of growth of B. coagulans (50 to 55 degrees C, pH 5.0) are very similar to that of commercially developed fungal cellulases (50 degrees C; pH 4.8). Due to this match, simultaneous saccharification and fermentation (SSF) of cellulose to products by B. coagulans is expected to require less cellulase than needed if the SSF is conducted at a sub-optimal temperature, such as 30 degrees C, the optimum for yeast, the main biocatalyst used by the ethanol industry. To fully exploit B. coagulans as a platform organism, we have developed an electroporation method to transfer plasmid DNA into this genetically recalcitrant bacterium. We also constructed a B. coagulans/E. coli shuttle vector, plasmid pMSR10 that contains the rep region from a native plasmid (pMSR0) present in B. coagulans strain P4-102B. The native plasmid, pMSR0 (6823bp), has 9 ORFs, and replicates by rolling-circle mode of replication. Plasmid pNW33N, developed for Geobacillus stearothermophilus, was also transformed into this host and stably maintained while several other Bacillus/Escherichia coli shuttle vector plasmids were not transformed into B. coagulans. The transformation efficiency of B. coagulans strain P4-102B using the plasmids pNW33N or pMSR10 was about 1.5x10(16) per mole of DNA. The availability of shuttle vectors and an electroporation method is expected to aid in genetic and metabolic engineering of B. coagulans.
journal_name
Plasmidjournal_title
Plasmidauthors
Rhee MS,Kim JW,Qian Y,Ingram LO,Shanmugam KTdoi
10.1016/j.plasmid.2006.11.006subject
Has Abstractpub_date
2007-07-01 00:00:00pages
13-22issue
1eissn
0147-619Xissn
1095-9890pii
S0147-619X(06)00113-2journal_volume
58pub_type
杂志文章相关文献
PLASMID文献大全abstract::In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restric...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90007-y
更新日期:1990-11-01 00:00:00
abstract::A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.1272
更新日期:1997-01-01 00:00:00
abstract::Rolling circle-replicating plasmids constitute a vast family that is particularly abundant in, but not exclusive of, Gram-positive bacteria. These plasmids are constructed as cassettes that harbor genes involved in replication and its control, mobilization, resistance determinants and one or two origins of lagging str...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2014.05.004
更新日期:2014-07-01 00:00:00
abstract::The ability of some bacteria to grow in the presence of high concentrations of tellurium compounds has been recognized for almost 100 years. Since then, interest in this phenomenon has generated a slow but steady trickle of literature. In the past few years, the use of modern techniques in molecular biology has led to...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/0147-619x(92)90006-v
更新日期:1992-01-01 00:00:00
abstract::DNA sequence polymorphism was determined for the microcin V gene cluster encoded on the microcin V plasmids of 12 natural isolates of Escherichia coli. These microcin V gene clusters are similar in DNA sequence, with only 10 of the 683 bp polymorphic. Further, the levels and patterns of microcin V gene cluster polymor...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2001.1525
更新日期:2001-07-01 00:00:00
abstract::Nonviral gene delivery has gained a lot of interest as a promising approach for gene therapy. Despite intensive studies and much progress the outcome of nonviral vectors has remained significantly weaker than that of viral vectors. A weak transfection efficiency of nonviral gene transfection is still limiting their in...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2009.09.003
更新日期:2010-01-01 00:00:00
abstract::The Lactococcus lactis group II intron (Ll.ltrB) retrohomes into the ltrB gene at high efficiency. To date, the critical DNA bases recognized in vivo by the Ll.ltrB ribonucleoprotein (RNP) have been exclusively elucidated in Escherichia coli. However, recent evidence indicates host-dependant differences in Ll.ltrB mob...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2007.02.003
更新日期:2007-09-01 00:00:00
abstract::The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1008
更新日期:1995-01-01 00:00:00
abstract::Pseudomonas syringae pv. phaseolicola (Pph) strain 1302A, a causative agent of halo blight in the common bean Phaseolus vulgaris, contains four native plasmids designated pAV505 (150 kb), pAV506 (50 kb), pAV507 (47 kb) and pAV508 (42 kb). Pph 1302A also contains a 106 kb genomic island PPHGI-1 which shares features wi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2013.07.002
更新日期:2013-11-01 00:00:00
abstract::CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.06.002
更新日期:2015-09-01 00:00:00
abstract::Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1289
更新日期:1997-01-01 00:00:00
abstract::The mercury(II)-resistance transposons Tn502 and Tn512 were sequenced and shown to be members of the Tn5053 family. They are currently the sole representatives from the clinical setting and were obtained from geographically disparate Pseudomonas aeruginosa strains. The family is comprised of six novel transposons that...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2010.08.003
更新日期:2011-01-01 00:00:00
abstract::The construction of a set of new plasmids that are suitable as general cloning vectors in Escherichia coli and Agrobacterium tumefaciens is described. Plasmid pUCD2 is amplifiable in E. coli, replicates in a wide range of gram-negative hosts and contains a number of useful restriction endonuclear cleavage sites and an...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(84)90057-x
更新日期:1984-09-01 00:00:00
abstract::The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1993.1045
更新日期:1993-09-01 00:00:00
abstract::Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(90)90035-b
更新日期:1990-03-01 00:00:00
abstract::This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2004.12.008
更新日期:2005-03-01 00:00:00
abstract::A collection of 111 commensal Escherichia coli isolated from 84 faecal samples from healthy Australian adults were screened using PCR-based replicon typing. Each isolate represented a distinct strain found in a particular faecal sample. Fifty-one isolates were resistant to one or more of 12 antibiotics tested. FII and...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2015.03.005
更新日期:2015-07-01 00:00:00
abstract::Tn4555 is a 12.1-kb Bacteroides antibiotic resistance transposon representative of a novel class of transmissible genetic elements that can be transferred by resident conjugative tetracycline resistance transposons (Tc(r)-elements) but are not capable of self-transfer. Previously it was shown that Tn4555 transposes by...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1401
更新日期:1999-07-01 00:00:00
abstract::The replication (rep) regions of small plasmids from three Sinorhizobium meliloti strains were cloned by marker rescue. Two unique replication regions were identified, one of which was common to two different strains. Plasmid pBB83 carried a 7.2 kbp rep region from a 42 kbp plasmid, and pBB84 carried a 4.5 kbp rep reg...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2005.08.003
更新日期:2006-03-01 00:00:00
abstract::The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2003.09.002
更新日期:2004-01-01 00:00:00
abstract::Sphingomonas sp. A1 (strain A1) is capable of directly incorporating macromolecules (e.g., alginate) through the specialized import system--"super-channel." Here, we report the complete DNA sequence and genetic organization of plasmid pA1 from strain A1. Nucleotide sequence analysis revealed that pA1 comprises 46,557 ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2005.12.002
更新日期:2006-07-01 00:00:00
abstract::To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/j.plasmid.2008.09.006
更新日期:2009-01-01 00:00:00
abstract::The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(89)90007-3
更新日期:1989-11-01 00:00:00
abstract::Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(03)00011-8
更新日期:2003-03-01 00:00:00
abstract::The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1399
更新日期:1999-05-01 00:00:00
abstract::Tn1935, a 23.5-kb transposon mediating resistance to ampicillin, kanamycin, mercury, spectinomycin, and sulfonamide was isolated from pZM3, an IncFIme virulence plasmid from Salmonella wien. Tn1935 possesses the entire sequence of Tn21 and contains two additional DNA segments of 0.95 and 2.7 kb carrying the ampicillin...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90028-5
更新日期:1988-11-01 00:00:00
abstract::Borrelia species comprise a unique genus of bacterial pathogens. These organisms contain a segmented genome with up to two dozen plasmids ranging in size from 5 kb up to about 200 kb. The plasmids have also been referred to as mini-chromosomes or essential genetic elements, as some of them carry information important ...
journal_title:Plasmid
pub_type: 杂志文章,评审
doi:10.1016/j.plasmid.2013.05.002
更新日期:2013-09-01 00:00:00
abstract::A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(88)90061-3
更新日期:1988-01-01 00:00:00
abstract::Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1500
更新日期:2001-01-01 00:00:00
abstract::A phagemid vector library for cloning DNA with four nucleotide 5' or 3' overhangs has been constructed. This library is based on the pT7T3 vector (Pharmacia) which is a modification of the phagemid pTZ18U vector. We have chosen pT7T3 as the parent vector because it can be used for Sanger's dideoxy sequencing and for t...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/0147-619x(91)90038-x
更新日期:1991-07-01 00:00:00