A novel insertion sequence in the cryptic plasmid of Neisseria gonorrhoeae may alter the B protein at the translational level.

abstract：:The nucleotide sequence of a smallest cryptic plasmid pRK10 of Serratia marcescens ACE2 was determined. When compared to the all other plasmids reported so far from S. marcescens in sizes of over 70kb, pRK10 is only 4241bp long with 53% G+C content and has five coding sequences representing a coding percentage of 65.4...

journal_title：Plasmid

authors： Syed Ibrahim K,Bakkiyaraj D,James R,Ganesh Babu T,Karutha Pandian ST

更新日期：2009-11-01 00:00:00

abstract：:Almost 200 scientists from America, Europe, Asia, Australia, and Africa participated in the Plasmid Biology 2004 meeting, which was organized between 15th and 21st September 2004 in Kanoni (Corfu island), Greece. Various aspects of biology of plasmids and other mobile genetic elements were discussed during the meeting...

journal_title：Plasmid

authors： Wegrzyn G

更新日期：2005-01-01 00:00:00

abstract：:A new cryptic plasmid from a multi-resistant, multi-plasmid clinical strain of Escherichia coli has been isolated. The sequence of the 4072-base-pair pIGWZ12 (GenBank Accession No. DQ311641) was determined and analyzed. Two open-reading frames that code for proteins involved in plasmid mobilization and initiation of r...

journal_title：Plasmid

authors： Zaleski P,Wolinowska R,Strzezek K,Lakomy A,Plucienniczak A

更新日期：2006-11-01 00:00:00

abstract：:The nucleotide sequence of a 3955-bp Helicobacter pylori plasmid, pHP51 was determined, and two open reading frames, ORF1 and ORF2, were identified. The deduced amino acid sequence of ORF1 was highly conserved (87-89%) among plasmid replication initiation proteins, RepBs. The function of ORF2 was not assigned because ...

journal_title：Plasmid

authors： Song JY,Choi SH,Byun EY,Lee SG,Park YH,Park SG,Lee SK,Kim KM,Park JU,Kang HL,Baik SC,Lee WK,Cho MJ,Youn HS,Ko GH,Bae DW,Rhee KH

更新日期：2003-09-01 00:00:00

abstract：:The promiscuous IncPα plasmids RK2 and R995 encode a broad-host-range partition system, whose essential components include the incC and korB genes and a DNA site (O(B)) to which the korB product binds. IncC2, the smaller of the two incC products, is sufficient for stabilization of R995ΔincC. It is a member of the type...

journal_title：Plasmid

authors： Siddique A,Figurski DH

更新日期：2012-09-01 00:00:00

abstract：:The region responsible for the maintenance of the prophage of bacteriophage P7 as a stable, unit-copy plasmid was isolated in a lambda att vector which lysogenizes Escherichia coli as a stable unit-copy plasmid under the control of the P7 replication origin. The P7 plasmid-maintenance region was shown to consist of ad...

journal_title：Plasmid

authors： Ludtke DN,Austin SJ

更新日期：1987-07-01 00:00:00

abstract：:Pinene is a monoterpene with wide industrial applications, especially as a promising high energy-density jet fuel. Traditional production of pinene on an industrial scale is material consumptive and has a low yield. As an alternative, microbial organisms have been engineered though advanced synthetic biological techni...

journal_title：Plasmid

authors： Bao SH,Zhang DY,Meng E

更新日期：2019-09-01 00:00:00

abstract：:Results of restriction endonuclease analysis and Southern blot hybridization suggest that the R-plasmids from Vibrio anguillarum strains isolated in Japan can be divided into at least four groups of homology depending on the time of their isolation and geographical source. Molecular cloning experiments allowed identif...

journal_title：Plasmid

authors： Mitoma Y,Aoki T,Crosa JH

更新日期：1984-11-01 00:00:00

abstract：:The construction of a set of new plasmids that are suitable as general cloning vectors in Escherichia coli and Agrobacterium tumefaciens is described. Plasmid pUCD2 is amplifiable in E. coli, replicates in a wide range of gram-negative hosts and contains a number of useful restriction endonuclear cleavage sites and an...

journal_title：Plasmid

authors： Close TJ,Zaitlin D,Kado CI

更新日期：1984-09-01 00:00:00

abstract：:Identification of the Sym plasmid in Rhizobium phaseoli strain RCC3622 is described. Introduction of this plasmid into R. trifolii or Agrobacterium tumefaciens strains resulted in bacteria capable of forming characteristic spherical root nodules on beans. This Sym plasmid, designated pSym9, was characterized as 275 MD...

journal_title：Plasmid

authors： Hooykaas PJ,den Dulk-Ras H,Regensburg-Tuïnk AJ,van Brussel AA,Schilperoort RA

更新日期：1985-07-01 00:00:00

abstract：:Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...

journal_title：Plasmid

authors： Banerjee H,Verma M

更新日期：2000-03-01 00:00:00

abstract：:Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...

journal_title：Plasmid

authors： Bahk JD,Kioka N,Sakai H,Komano T

更新日期：1988-11-01 00:00:00

abstract：:In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restric...

journal_title：Plasmid

authors： Labes G,Simon R

更新日期：1990-11-01 00:00:00

abstract：:Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...

journal_title：Plasmid

authors： Miller CA 3rd,Tan X,Wilson M,Bhattacharyya S,Ludwig S

更新日期：2010-03-01 00:00:00

abstract：:The effects of the single-strand origins (SSOs) of plasmid pMV158 on (i) the conversion of its single-stranded (ss) replication intermediates to double-stranded (ds) plasmid DNA and (ii) its maintenance were analyzed. The rolling-circle plasmid pMV158, which replicates via ssDNA intermediates, contains two single-stra...

journal_title：Plasmid

authors： Meijer WJ,van der Lelie D,Venema G,Bron S

更新日期：1995-03-01 00:00:00

abstract：:Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...

journal_title：Plasmid

authors： D'Haeze W,Verplancke C,Mironov V,Holsters M

更新日期：2002-03-01 00:00:00

abstract：:Isolates of Rhodococcus equi from pneumonic foals possess an 85- or 90-kb virulence-associated plasmid. A prominent, thermoregulated surface antigen, VapA, encoded by these plasmids is thought to be important in virulence. A 135-kb fragment containing the origin of replication of R. equi strain 103 virulence-associate...

journal_title：Plasmid

authors： Zheng H,Tkachuk-Saad O,Prescott JF

更新日期：1997-01-01 00:00:00

abstract：:This review provides a historical perspective of the major findings that contributed to our current understanding of plasmid rolling-circle (RC) replication. Rolling-circle-replicating (RCR) plasmids were discovered approximately 20 years ago. The first of the RCR plasmids to be identified were native to Gram-positive...

journal_title：Plasmid

authors： Khan SA

更新日期：2005-03-01 00:00:00

abstract：:pSK41 is a prototypical 46-kb conjugative multiresistance plasmid of Staphylococcus aureus. The pSK41 replication initiation protein (Rep) is rate-limiting for plasmid replication, and its expression is negatively regulated by a small, non-coding antisense transcript, RNAI, that is complementary to the rep mRNA leader...

journal_title：Plasmid

authors： Kwong SM,Firth N

更新日期：2015-03-01 00:00:00

abstract：:Nucleotide sequence analysis of the Tn4451-deletion derivatives, pJIR47 and pJIR86, which were derived from Escherichia coli and Clostridium perfringens, respectively, showed that the deletion events that led to the formation of these plasmids were identical and precise. The results also showed that the termini of thi...

journal_title：Plasmid

authors： Abraham LJ,Rood JI

更新日期：1988-03-01 00:00:00

abstract：:From Enterococcus faecalis cells containing random chromosomal insertions of Tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. The phage-resistant strains all showed decreased recipient ability (Con-) in broth matings with donors carrying pheromone-inducible plasmids. Thes...

journal_title：Plasmid

authors： Trotter KM,Dunny GM

更新日期：1990-07-01 00:00:00

abstract：:Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...

journal_title：Plasmid

authors： Jo C,Jo SA

更新日期：2001-01-01 00:00:00

abstract：:The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...

journal_title：Plasmid

authors： Shammat IM,Welker DL

更新日期：1999-05-01 00:00:00

abstract：:A number of plasmid vectors were constructed for high-level gene expression in the dimorphic gram-negative bacterium Caulobacter crescentus. These vectors incorporate the transcription and translation initiation regions of the C. crescentus CB15A rsaA gene, which codes for the abundantly synthesized protein comprising...

journal_title：Plasmid

authors： Bingle WH,Smit J

更新日期：1990-09-01 00:00:00

abstract：:A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...

journal_title：Plasmid

authors： Zhong Z,Caspi R,Mincer T,Helinski D,Knauf V,Boardman K,Wilkinson JE,Shea T,DeLoughery C,Toukdarian A

更新日期：2002-01-01 00:00:00

abstract：:Most Vibrio fischeri strains isolated from the Euprymna scolopes light organ carry plasmids, often including both a large (>40kb) plasmid, and one or more small (<12kb) plasmids. The large plasmids share homology with pES100, which is the lone plasmid in V. fischeri type strain ES114. pES100 appears to encode a conjug...

journal_title：Plasmid

authors： Dunn AK,Martin MO,Stabb EV

更新日期：2005-09-01 00:00:00

abstract：:A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...

journal_title：Plasmid

authors： Qiao M,Gao C,Cai B,Zhang X,Wang S

更新日期：1992-05-01 00:00:00

abstract：:Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...

journal_title：Plasmid

authors： Boe L,Rasmussen KV

更新日期：1996-11-01 00:00:00

abstract：:Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...

journal_title：Plasmid

authors： Peijnenburg AC,Breed PV,Bron S,Venema G

更新日期：1989-05-01 00:00:00

abstract：:A new insertion variant of IS231 family, designated ISBth4, was identified from Bacillus thuringiensis MEX312. ISBth4 is 2046bp in length and is delimited by two 17bp inverted repeats (IR) with one mismatch, flanked by two perfect 11bp direct repeats (DR). ISBth4 contains two open reading frames (ORFs), ORF1 and ORF2,...

journal_title：Plasmid

authors： Wang J,Chaudhuri RR,Ellar DJ

更新日期：2010-01-01 00:00:00