Abstract:
:We have constructed a pUC19-based multipurpose ATG vector in Schizosaccharomyces pombe with higher copy number and mitotic stability possible with commonly used vectors. The vector, having an NdeI site in its polylinker to provide ATG site for expression, carries a greatly truncated version of URA3 gene, URA3m, of Saccharomyces cerevisiae as a selection marker. In addition, it contains the mat2P-right flank region (mat2P-RF) of S. pombe as an autonomous replicating sequence (ARS) and a polylinker with wider choice of restriction sites. While URA3m confers an increase in plasmid copy number up to 200 copies/cell, mat2P-RF imparts greater mitotic stability than the standard ars1 element of S. pombe. Finally, the vector also includes the transcription termination signal of the nmt1 gene (Tnmt1). This basic vector should serve as a versatile tool for studies of gene function in S. pombe.
journal_name
Plasmidjournal_title
Plasmidauthors
Verma HK,Singh Jdoi
10.1016/j.plasmid.2012.07.001subject
Has Abstractpub_date
2012-11-01 00:00:00pages
186-94issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(12)00078-9journal_volume
68pub_type
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