Restriction map of virulence plasmid in Yersinia enterocolitica O:3.


:Restriction map of the 72-kb virulence plasmid isolated from Yersinia enterocolitica O:3 was generated using EcoRI, BamHI, HindIII, and XbaI restriction enzymes. The mapping was done after cloning all of the 13 BamHI fragments of the plasmid in Escherichia coli. In addition, the restriction enzyme analysis revealed two types of virulence plasmids (types I and II) in Y. enterocolitica O:3. No functional differences between the strains bearing type I or type II plasmid were observed.






Pulkkinen L,Granberg I,Granfors K,Toivanen A




Has Abstract


1986-11-01 00:00:00














  • A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184.

    abstract::The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that...


    pub_type: 杂志文章


    authors: Chakravartty V,Cronan JE

    更新日期:2015-09-01 00:00:00

  • Stacking of three different restriction and modification systems in Lactococcus lactis by cotransformation.

    abstract::Four plasmids encoding restriction and modification (R/M) systems are described that are different in the specificity of their restrictive activity toward the small isometric phage p2 and prolate phage c2. The R/M plasmids were cotransformed into Lactococcus lactis MG1363 with pVS2, encoding resistance to chlorampheni...


    pub_type: 杂志文章


    authors: Josephsen J,Klaenhammer T

    更新日期:1990-01-01 00:00:00

  • Cloning of Bacteroides fragilis plasmid genes affecting metronidazole resistance and ultraviolet survival in Escherichia coli.

    abstract::Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and...


    pub_type: 杂志文章


    authors: Wehnert GU,Abratt VR,Goodman HJ,Woods DR

    更新日期:1990-03-01 00:00:00

  • Peripheral sequences of the Serratia entomophila pADAP virulence-associated region.

    abstract::Some strains of the Enterobacteriaceae Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The genes responsible for this disease reside on a large, 155-kb plasmid designated amber disease-as...


    pub_type: 杂志文章


    authors: Hurst MR,O'Callaghan M,Glare TR

    更新日期:2003-11-01 00:00:00

  • Cloning and sequence analysis of a novel insertion element from plasmids harbored by the carbofuran-degrading bacterium, Sphingomonas sp. CFO6.

    abstract::Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...


    pub_type: 杂志文章


    authors: Feng X,Ou LT,Ogram A

    更新日期:1997-01-01 00:00:00

  • Compatible Dictyostelium mucoroides nuclear plasmids Dmp1 and Dmp2 both belong to the Ddp1 plasmid family.

    abstract::Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...


    pub_type: 杂志文章


    authors: Kiyosawa H,Hughes JE,Welker DL

    更新日期:1994-03-01 00:00:00

  • Comparative characterization of repABC-type replicons of Paracoccus pantotrophus composite plasmids.

    abstract::The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...


    pub_type: 杂志文章


    authors: Bartosik D,Baj J,Piechucka E,Waker E,Wlodarczyk M

    更新日期:2002-09-01 00:00:00

  • Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays.

    abstract::Single plasmids designed to express the six human type I steroid hormone receptors and detect signaling activity are described in this report. These stably replicating plasmids reported ligand-induced transcriptional activation via lacZ assays in Baker's yeast (Saccharomyces cerevisiae). The ligand concentrations need...


    pub_type: 杂志文章


    authors: Miller CA 3rd,Tan X,Wilson M,Bhattacharyya S,Ludwig S

    更新日期:2010-03-01 00:00:00

  • A physical and genetic map of the IncFI plasmid ColV2-K94.

    abstract::A restriction enzyme map of the IncFI plasmid ColV2-K94 was generated using EcoRI, BamHI, HindIII, and XhoI; the genetic features of this element were then mapped from previous heteroduplex studies. ...


    pub_type: 杂志文章


    authors: Weber PC,Palchaudhuri S

    更新日期:1985-05-01 00:00:00

  • Molecular relationships between pseudomonas INC P-9 degradative plasmids TOL, NAH, and SAL.

    abstract::We have examined the extent to which the degradative plasmids SAL, NAH, and TOL of the Inc P-9 incompatibility group share common DNA sequences. The homology we observe using 32P-labeled SAL and NAH DNA probes can be assigned to six regions of the TOL (pWWO) restriction endonuclease cleavage map. At least three of the...


    pub_type: 杂志文章


    authors: Lehrbach PR,McGregor I,Ward JM,Broda P

    更新日期:1983-09-01 00:00:00

  • Search for a novel killer toxin in yeast Pichia pastoris.

    abstract::Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...


    pub_type: 杂志文章


    authors: Banerjee H,Verma M

    更新日期:2000-03-01 00:00:00

  • Characterization of ISBth4, a functional new IS231 variant from Bacillus thuringiensis MEX312.

    abstract::A new insertion variant of IS231 family, designated ISBth4, was identified from Bacillus thuringiensis MEX312. ISBth4 is 2046bp in length and is delimited by two 17bp inverted repeats (IR) with one mismatch, flanked by two perfect 11bp direct repeats (DR). ISBth4 contains two open reading frames (ORFs), ORF1 and ORF2,...


    pub_type: 杂志文章


    authors: Wang J,Chaudhuri RR,Ellar DJ

    更新日期:2010-01-01 00:00:00

  • The bacteriophage resistance plasmid pTR2030 forms high-molecular-weight multimers in lactococci.

    abstract::Lactococcus lactis ME2 can transfer a 46-kb plasmid, pTR2030, which encodes abortive phage infection (Hsp) and restriction/modification (R/M) activities. pTR2030 can be detected as a monomeric plasmid in transconjugants at low copy number, but not in ME2. pTR2030-specific probes were cloned and used to determine the l...


    pub_type: 杂志文章


    authors: Hill C,Miller LA,Klaenhammer TR

    更新日期:1991-03-01 00:00:00

  • Organization of the horizontally transferred pheBA operon and its adjacent genes in the genomes of eight indigenous Pseudomonas strains.

    abstract::Horizontal transfer of genes encoding phenol degradation (pheBA) in the environment has been previously described. Complete or partial phe-operon was redetected in plasmids of several indigenous Pseudomonas strains isolated from the river water. The sequences of up- and downstream regions of the acquired phe-DNA in ei...


    pub_type: 杂志文章


    authors: Peters M,Tomikas A,Nurk A

    更新日期:2004-11-01 00:00:00

  • Transcription start sites in the promoter region of the Escherichia coli pcnB (plasmid copy number) gene coding for poly(A) polymerase I.

    abstract::Escherichia coli pcnB gene was discovered as a regulator of ColE1 plasmid copy number about 20 years ago. Further studies indicated that this gene codes for poly(A) polymerase I (PAP I). However, although importance of RNA polyadenylation has been demonstrated and biochemical properties of PAP I have been studied exte...


    pub_type: 杂志文章


    authors: Jasiecki J,Wegrzyn G

    更新日期:2006-03-01 00:00:00

  • CoIV plasmids pCoIV-B188 and pCoIV-K30: genetic maps according to restriction enzyme sites and landmark phenotypic characteristics.

    abstract::The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...


    pub_type: 杂志文章


    authors: Waters VL,Perez-Casal JF,Crosa JH

    更新日期:1989-11-01 00:00:00

  • Plasmid distribution and analyses in Rhodopseudomonas sphaeroides.

    abstract::Ten strains of Rhodopseudomonas sphaeroides were analyzed by agarose gel electrophoresis for plasmid DNA content and, by filter-hybridizations, for their molecular relationships. All strains examined contained at least one plasmid. Several strains carried as many as six different plasmid species with sizes ranging fro...


    pub_type: 杂志文章


    authors: Fornari CS,Watkins M,Kaplan S

    更新日期:1984-01-01 00:00:00

  • New pSC101-derivative cloning vectors with elevated copy numbers.

    abstract::Mutations that increase the copy number of the pSC101 replicon have been used for construction of new cloning vectors. Replacement of glutamate at position 93 in RepA yields plasmids that replicate at medium (27 copies/cell) and high (approximately 240 copies/cell) copy numbers. Based on the crystal structure of RepE,...


    pub_type: 杂志文章


    authors: Peterson J,Phillips GJ

    更新日期:2008-05-01 00:00:00

  • Restriction endonuclease mapping of R27 (TP117), an incompatibility group HI subgroup 1 plasmid from Salmonella typhimurium.

    abstract::A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...


    pub_type: 杂志文章


    authors: Taylor DE,Brose EC

    更新日期:1985-01-01 00:00:00

  • Characterization of the cryptic plasmid pCC1 from Corynebacterium callunae and its use for vector construction.

    abstract::The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...


    pub_type: 杂志文章


    authors: Venkova-Canova T,Pátek M,Nesvera J

    更新日期:2004-01-01 00:00:00

  • Trapping developmental promoters in Dictyostelium.

    abstract::Recently an insertional mutagenesis procedure has been developed to permit cloning of genes affected in developmental mutants of Dictyostelium discoideum (Kuspa and Loomis, Proc. Natl. Acad. Sci. USA 89, 8803-8807, 1992). In this procedure a plasmid bearing the URA (pyr5-6) gene is linearized with a restriction enzyme...


    pub_type: 杂志文章


    authors: Chang WT,Gross JD,Newell PC

    更新日期:1995-11-01 00:00:00

  • Sequence analysis of two cryptic plasmids from an agricultural isolate of Campylobacter coli.

    abstract::As part of a study identifying plasmids in Campylobacter, we isolated and sequenced two novel cryptic plasmids from an agricultural isolate of Campylobacter coli. The larger of the two plasmids, p3384, is 3316 bp in length and has a G+C content of 31.18%. A typical origin of replication consisting of five iterons was ...


    pub_type: 杂志文章


    authors: Jesse TW,Pittenger-Alley LG,Englen MD

    更新日期:2006-01-01 00:00:00

  • Tailor-made sRNAs: a plasmid tool to control the expression of target mRNAs in Pseudomonas putida.

    abstract::Pseudomonas putida is a highly attractive production system for industrial needs. However, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a plasmid expressing a small RNA-based system with the potential to be used for di...


    pub_type: 杂志文章


    authors: Apura P,Saramago M,Peregrina A,Viegas SC,Carvalho SM,Saraiva LM,Arraiano CM,Domingues S

    更新日期:2020-05-01 00:00:00

  • Characterization of pPT23B, the plasmid involved in syringolide production by Pseudomonas syringae pv. tomato PT23.

    abstract::Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...


    pub_type: 杂志文章


    authors: Murillo J,Shen H,Gerhold D,Sharma A,Cooksey DA,Keen NT

    更新日期:1994-05-01 00:00:00

  • The linear plasmid SCP1 of Streptomyces coelicolor A3(2) possesses a centrally located replication origin and shows significant homology to the transposon Tn4811.

    abstract::The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the genetically more studied linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the help of an ordered co...


    pub_type: 杂志文章


    authors: Redenbach M,Bibb M,Gust B,Seitz B,Spychaj A

    更新日期:1999-11-01 00:00:00

  • High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors.

    abstract::We describe here the construction of Gateway-compatible vectors, pBGP1-DEST and pPICZα-DEST, for rapid and convenient preparation of expression plasmids for production of secretory proteins in Pichia pastoris. Both vectors direct the synthesis of fusion proteins consisting of the N-terminal signal and pro-sequences of...


    pub_type: 杂志文章


    authors: Sasagawa T,Matsui M,Kobayashi Y,Otagiri M,Moriya S,Sakamoto Y,Ito Y,Lee CC,Kitamoto K,Arioka M

    更新日期:2011-01-01 00:00:00

  • Integration-proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains.

    abstract::An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...


    pub_type: 杂志文章


    authors: Hoang TT,Kutchma AJ,Becher A,Schweizer HP

    更新日期:2000-01-01 00:00:00

  • Complete nucleotide sequence of the self-transmissible TOL plasmid pD2RT provides new insight into arrangement of toluene catabolic plasmids.

    abstract::In the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pD2RT of Pseudomonas migulae strain D2RT isolated from Baltic Sea water. The pD2RT is 129,894 base pairs in size with an average G+C content of 53.75%. A total of 135 open reading frames (ORFs) were predicted to encode pro...


    pub_type: 杂志文章


    authors: Jutkina J,Hansen LH,Li L,Heinaru E,Vedler E,Jõesaar M,Heinaru A

    更新日期:2013-11-01 00:00:00

  • Carriage of type II toxin-antitoxin systems by the growing group of IncX plasmids.

    abstract::The stable maintenance of certain plasmids in bacterial populations has contributed significantly to the current worldwide antibiotic resistance (AbR) emergency. IncX plasmids, long underestimated in this regard, have achieved recent notoriety for their roles in transmission of resistance to carbapenem and colistin, t...


    pub_type: 杂志文章


    authors: Bustamante P,Iredell JR

    更新日期:2017-05-01 00:00:00

  • A stable Escherichia coli-Mycobacterium smegmatis plasmid shuttle vector containing the mycobacteriophage D29 origin.

    abstract::A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...


    pub_type: 杂志文章


    authors: David M,Lubinsky-Mink S,Ben-Zvi A,Ulitzur S,Kuhn J,Suissa M

    更新日期:1992-11-01 00:00:00