Restriction map of virulence plasmid in Yersinia enterocolitica O:3.


:Restriction map of the 72-kb virulence plasmid isolated from Yersinia enterocolitica O:3 was generated using EcoRI, BamHI, HindIII, and XbaI restriction enzymes. The mapping was done after cloning all of the 13 BamHI fragments of the plasmid in Escherichia coli. In addition, the restriction enzyme analysis revealed two types of virulence plasmids (types I and II) in Y. enterocolitica O:3. No functional differences between the strains bearing type I or type II plasmid were observed.






Pulkkinen L,Granberg I,Granfors K,Toivanen A




Has Abstract


1986-11-01 00:00:00














  • Tn4527, a Tp Sp/Sm transposon related to Tn7 and flanked by IS1.

    abstract::Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....


    pub_type: 杂志文章


    authors: Dumans AT,Carvalho MF,Coelho A

    更新日期:1989-11-01 00:00:00

  • A physical and genetic map of the IncFI plasmid ColV2-K94.

    abstract::A restriction enzyme map of the IncFI plasmid ColV2-K94 was generated using EcoRI, BamHI, HindIII, and XhoI; the genetic features of this element were then mapped from previous heteroduplex studies. ...


    pub_type: 杂志文章


    authors: Weber PC,Palchaudhuri S

    更新日期:1985-05-01 00:00:00

  • A novel plasmid, pSAA0430-08, from Streptococcus anginosus subsp. anginosus strain 0430-08.

    abstract::Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence ...


    pub_type: 杂志文章


    authors: Tabata A,Deutsch D,Otsuka S,Verratti K,Tomoyasu T,Nagamune H,Fischetti VA

    更新日期:2018-01-01 00:00:00

  • Comparative characterization of repABC-type replicons of Paracoccus pantotrophus composite plasmids.

    abstract::The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...


    pub_type: 杂志文章


    authors: Bartosik D,Baj J,Piechucka E,Waker E,Wlodarczyk M

    更新日期:2002-09-01 00:00:00

  • The use of mini-Gal plasmids for rapid incompatibility grouping of conjugative R plasmids.

    abstract::The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their ...


    pub_type: 杂志文章


    authors: Davey RB,Bird PI,Nikoletti SM,Praszkier J,Pittard J

    更新日期:1984-05-01 00:00:00

  • Resistance to imidazoles and triazoles in Saccharomyces cerevisiae as a new dominant marker.

    abstract::The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...


    pub_type: 杂志文章


    authors: Doignon F,Aigle M,Ribereau-Gayon P

    更新日期:1993-11-01 00:00:00

  • Eukaryotic expression vectors that replicate to low copy number in bacteria: transient expression of the Menkes protein.

    abstract::A set of low copy number plasmid vectors for mammalian gene expression has been constructed. These vectors are derived from the previously described bacterial low copy number expression vectors, pWSK29 and pWKS30, which are present at six to eight copies per cell. The new plasmids also have the following useful proper...


    pub_type: 杂志文章


    authors: Fontaine SL,Firth SD,Lockhart PJ,Paynter JA,Mercer JF

    更新日期:1998-01-01 00:00:00

  • Coupling sequences flanking Tn916 do not determine the affinity of binding of integrase to the transposon ends and adjacent bacterial DNA.

    abstract::Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integra...


    pub_type: 杂志文章


    authors: Pethel B,Churchward G

    更新日期:2000-03-01 00:00:00

  • Sample purification improves the analysis of nonviral in vivo gene transfection.

    abstract::Nonviral gene delivery has gained a lot of interest as a promising approach for gene therapy. Despite intensive studies and much progress the outcome of nonviral vectors has remained significantly weaker than that of viral vectors. A weak transfection efficiency of nonviral gene transfection is still limiting their in...


    pub_type: 杂志文章


    authors: Soininen P,Hanzlíková M,Paukkunen M,Lecklin A,Männistö PT,Raasmaja A

    更新日期:2010-01-01 00:00:00

  • New plasmid vector and host system for genetic engineering in Bacillus sphaericus.

    abstract::A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...


    pub_type: 杂志文章


    authors: Qiao M,Gao C,Cai B,Zhang X,Wang S

    更新日期:1992-05-01 00:00:00

  • Evidence that mutacin II production is not mediated by a 5.6-kb plasmid in Streptococcus mutans.

    abstract::Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...


    pub_type: 杂志文章


    authors: Caufield PW,Shah G,Hollingshead SK,Parrot M,Lavoie MC

    更新日期:1990-09-01 00:00:00

  • Isolation of thermosensitive mutants of Yersinia enterocolitica by transposon insertion.

    abstract::During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...


    pub_type: 杂志文章


    authors: Yamamoto T,Hanawa T,Murayama SY,Ogata S

    更新日期:1994-09-01 00:00:00

  • Suggestions as to quantitative measurements of plasmid loss.

    abstract::Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...


    pub_type: 杂志文章


    authors: Boe L,Rasmussen KV

    更新日期:1996-11-01 00:00:00

  • Construction of pOGOduet - An inducible, bicistronic vector for synthesis of recombinant proteins in Corynebacterium glutamicum.

    abstract::The Gram-positive Corynebacterium glutamicum is widely known for its application in the industrial production of amino acids and as a non-pathogenic model organism for cell wall biosynthesis in the group of CMN bacteria. For biotechnological and physiological studies often co-expression of recombinant genes is require...


    pub_type: 杂志文章


    authors: Goldbeck O,Seibold GM

    更新日期:2018-01-01 00:00:00

  • Inhibitory effect of UvrD and DinG on the replication of ColE1-derived plasmids in Escherichia coli.

    abstract::CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...


    pub_type: 杂志文章


    authors: Kang N,Choi E,Kim SG,Hwang J

    更新日期:2015-09-01 00:00:00

  • A novel insertion sequence in the cryptic plasmid of Neisseria gonorrhoeae may alter the B protein at the translational level.

    abstract::A variant of the cryptic plasmid of Neisseria gonorrhoeae, 4.4 kb in size, was isolated and characterized at the molecular level. This variant harbored a 156-bp insertion which was located between coordinates 3134 and 3135 within the putative cppB gene using the 4.2-kb cryptic plasmid, pJD1, as a reference. The insert...


    pub_type: 杂志文章


    authors: Roy RN,Bigelow N,Dillon JA

    更新日期:1988-01-01 00:00:00

  • Isolation and characterization of an extrachromosomal element from Nocardia mediterranei.

    abstract::Strain LBG A3136 of Nocardia mediterranei (ETH Collection) was found to contain a low-copy-number covalently closed circular extrachromosomal element, pMEA 100, which could only be isolated from mycelium grown on agar plates. pMEA 100 could not be isolated from the closely related strain ATCC 13685. Hybridization expe...


    pub_type: 杂志文章


    authors: Moretti P,Hintermann G,Hütter R

    更新日期:1985-09-01 00:00:00

  • The use of the replication region of plasmid pRS7 from Oenococcus oeni as a putative tool to generate cloning vectors for lactic acid bacteria.

    abstract::A chimeric plasmid, pRS7Rep (6.1 kb), was constructed using the replication region of pRS7, a large plasmid from Oenococcus oeni, and pEM64, a plasmid derived from pIJ2925 and containing a gene for resistance to chloramphenicol. pRS7Rep is a shuttle vector that replicates in Escherichia coli using its pIJ2925 componen...


    pub_type: 杂志文章


    authors: Rodríguez MC,Alegre MT,Martín MC,Mesas JM

    更新日期:2015-01-01 00:00:00

  • Nucleotide sequence and analysis of pBL1, a bacteriocin-producing plasmid from Lactococcus lactis IPLA 972.

    abstract::The complete sequence of the 10.9-kbp bacteriocinogenic plasmid pBL1 from Lactococcus lactis subsp. lactis IPLA 972 has been determined. Thirteen ORFs were encountered, of which 5 were incomplete. pBL1 proved to be a narrow-host-range plasmid which replicates neither in Bacilus subtilis nor in Lactobacillus spp. The s...


    pub_type: 杂志文章


    authors: Sánchez C,Hernández de Rojas A,Martínez B,Argüelles ME,Suárez JE,Rodríguez A,Mayo B

    更新日期:2000-11-01 00:00:00

  • Plasmid deletion formation in recE4 and addB72 mutants of Bacillus subtilis.

    abstract::Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...


    pub_type: 杂志文章


    authors: Peijnenburg AC,Breed PV,Bron S,Venema G

    更新日期:1989-05-01 00:00:00

  • Inclusion of a matrix-attached region in a 7SK pol III vector increases the efficiency of shRNA-mediated gene silencing in embryonic carcinoma cells.

    abstract::RNA interference is a widely used tool for analysis of gene function in mammalian cells. Stable knockdown of specific target genes can be maintained in cell lines and live organisms using vector-based delivery of short hairpins (shRNAs) driven by RNA polymerase III promoters. Here we describe a vector incorporating th...


    pub_type: 杂志文章


    authors: Sotirova VN,Calciano MA,Krueger W,Lalande M

    更新日期:2006-05-01 00:00:00

  • Construction of a new polycistronic vector for over-expression and rapid purification of human hemoglobin.

    abstract::To facilitate the study of the structure-function relationship of human hemoglobin (Hb A), we have developed a new hemoglobin expression vector, pGEX6P-alpha-[SD]-beta. This vector allows the co-expression of alpha-Hb as a fusion protein with Glutathione S-Transferase (GST-alpha-Hb) and beta-Hb with an additional meth...


    pub_type: 杂志文章


    authors: Domingues E,Brillet T,Vasseur C,Agier V,Marden MC,Baudin-Creuza V

    更新日期:2009-01-01 00:00:00

  • Widespread occurrence of the tfd-II genes in soil bacteria revealed by nucleotide sequence analysis of 2,4-dichlorophenoxyacetic acid degradative plasmids pDB1 and p712.

    abstract::Variovorax sp. strain DB1 and Pseudomonas pickettii strain 712 are 2,4-dicholorophenoxy-acetic acid (2,4-D)-degrading bacteria, which were isolated from agricultural soils in Republic of Korea and USA, respectively. Each strain harbors a 2,4-D degradative plasmid and is able to utilize 2,4-D as the sole source of carb...


    pub_type: 杂志文章


    authors: Kim DU,Kim MS,Lim JS,Ka JO

    更新日期:2013-05-01 00:00:00

  • Genetic structure and transcriptional analysis of a mobilizable, antibiotic resistance transposon from Bacteroides.

    abstract::Tn4555 is a 12.1-kb Bacteroides antibiotic resistance transposon representative of a novel class of transmissible genetic elements that can be transferred by resident conjugative tetracycline resistance transposons (Tc(r)-elements) but are not capable of self-transfer. Previously it was shown that Tn4555 transposes by...


    pub_type: 杂志文章


    authors: Tribble GD,Parker AC,Smith CJ

    更新日期:1999-07-01 00:00:00

  • Characterization of the cryptic plasmid pCC1 from Corynebacterium callunae and its use for vector construction.

    abstract::The complete nucleotide sequence of the cryptic plasmid pCC1 from Corynebacterium callunae (4109 bp) was determined. DNA sequence analysis revealed five open reading frames longer than 200 bp. One of the deduced polypeptides showed homology with the Rep proteins encoded by plasmids of the pIJ101/pJV1 family of plasmid...


    pub_type: 杂志文章


    authors: Venkova-Canova T,Pátek M,Nesvera J

    更新日期:2004-01-01 00:00:00

  • Pheromone-inducible expression vectors for fission yeast Schizosaccharomyces pombe.

    abstract::The fission yeast Schizosaccharomyces pombe is an attractive host for heterologous gene expression. However, expression systems for industrially viable large-scale fermentations are scarce. Several inducible expression vectors for S. pombe have been reported, with the strong thiamine-repressible nmt1+ promoter or deri...


    pub_type: 杂志文章


    authors: Hennig S,Hornauer N,Rödel G,Ostermann K

    更新日期:2018-01-01 00:00:00

  • Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces.

    abstract::pSAM2 is an 11-kb plasmid integrated in the Streptomyces ambofaciens ATCC23877 and ATCC15154 genomes and found additionally as a free replicon in an uv derivative. After transfer into S. ambofaciens DSM40697 (devoid of pSAM2) or into Streptomyces lividans, specific integration of pSAM2 occurred very efficiently. A 58-...


    pub_type: 杂志文章


    authors: Boccard F,Smokvina T,Pernodet JL,Friedmann A,Guérineau M

    更新日期:1989-01-01 00:00:00

  • Interaction between mitochondrial genes in yeast: evidence for novel box effect(s).

    abstract::The effects of mutations have been studied within the apocytochrome b gene on the processing of transcripts from the gene for subunit 1 of cytochrome c oxidase (coxI). Most mutations which affect the expression of the reading frame encoded by the fourth intron of the apocytochrome b gene (bI4) result in a failure to r...


    pub_type: 杂志文章


    authors: Hensgens LA,Van der Horst G,Grivell LA

    更新日期:1984-07-01 00:00:00

  • In vitro replication of cyanobacterial plasmids from Synechocystis PCC 6803.

    abstract::Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...


    pub_type: 杂志文章


    authors: Yang X,Daniell H,McFadden B

    更新日期:1994-09-01 00:00:00

  • IS1473, a putative insertion sequence identified in the plasmid pAO1 from Arthrobacter nicotinovorans: isolation, characterization, and distribution among Arthrobacter species.

    abstract::A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...


    pub_type: 杂志文章


    authors: Menéndez C,Igloi GL,Brandsch R

    更新日期:1997-01-01 00:00:00