Mutational and physiological analyses of plasmid pT181 functions expressing incompatibility.

abstract：:Homology was previously detected between the DNA restriction fragments containing Rhizobium meliloti nodulation genes and the 90-MDa plasmid, p90, of Azospirillum brasilense Sp7. Two DNA loci from Sp7 genome that complement mutations in the exopolysaccharide synthesis genes, exoB and exoC, of R. meliloti were also sho...

journal_title：Plasmid

authors： Onyeocha I,Vieille C,Zimmer W,Baca BE,Flores M,Palacios R,Elmerich C

更新日期：1990-05-01 00:00:00

abstract：:Taking advantage of the plaque morphology method, we detected two single-stranded initiation (ssi) signals in the plasmid pSY343; one was in the 170-nucleotide (nt) EcoRV-ThaI segment (170P), and the other was in the 93-nt DraI-FnuDII segment (93F), which were designated as ssiA and ssiB, respectively. We cloned the t...

journal_title：Plasmid

authors： Bahk JD,Kioka N,Sakai H,Komano T

更新日期：1988-11-01 00:00:00

abstract：:CspA has been identified as a major cold-shock protein in Escherichia coli. CspA binds to RNAs which are abnormally folded at low temperature and then acts as an RNA chaperone unfolding those RNAs. The dramatic expression of cspA at low temperature is contributed by posttranscriptional stability and robust translatabi...

journal_title：Plasmid

authors： Kang N,Choi E,Kim SG,Hwang J

更新日期：2015-09-01 00:00:00

abstract：:With the mutagenesis of specific, virulence-associated genes of Legionella pneumophila as the eventual goal, methods for gene transfer to these bacteria were developed. Following the observations of others that conjugative, broad-host-range plasmids could be transferred from Escherichia coli to L. pneumophila at low f...

journal_title：Plasmid

authors： Engleberg NC,Cianciotto N,Smith J,Eisenstein BI

更新日期：1988-07-01 00:00:00

abstract：:Previous explanations of why bacterial genes for certain "optional" traits tend to occur on plasmids rather than chromosomes are based on an outdated misunderstanding of natural selection. They also fail to explain why certain characters that are ubiquitous in some bacterial species tend to occur on plasmids. This pap...

journal_title：Plasmid

authors： Eberhard WG

更新日期：1989-05-01 00:00:00

abstract：:In the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pD2RT of Pseudomonas migulae strain D2RT isolated from Baltic Sea water. The pD2RT is 129,894 base pairs in size with an average G+C content of 53.75%. A total of 135 open reading frames (ORFs) were predicted to encode pro...

journal_title：Plasmid

authors： Jutkina J,Hansen LH,Li L,Heinaru E,Vedler E,Jõesaar M,Heinaru A

更新日期：2013-11-01 00:00:00

abstract：:Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...

journal_title：Plasmid

authors： Jo C,Jo SA

更新日期：2001-01-01 00:00:00

abstract：:A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...

journal_title：Plasmid

authors： David M,Lubinsky-Mink S,Ben-Zvi A,Ulitzur S,Kuhn J,Suissa M

更新日期：1992-11-01 00:00:00

abstract：:Tn4555 is a 12.1-kb Bacteroides antibiotic resistance transposon representative of a novel class of transmissible genetic elements that can be transferred by resident conjugative tetracycline resistance transposons (Tc(r)-elements) but are not capable of self-transfer. Previously it was shown that Tn4555 transposes by...

journal_title：Plasmid

authors： Tribble GD,Parker AC,Smith CJ

更新日期：1999-07-01 00:00:00

abstract：:The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae confers resistance to kanamycin (aphA-3), erythromycin (ermAM), and tetracycline (tetM). The 25.3-kb element is self-transferable to various gram-positive bacterial genera where it transposes. Tn1545 is also capable of transposition, but not of co...

journal_title：Plasmid

authors： Caillaud F,Carlier C,Courvalin P

更新日期：1987-01-01 00:00:00

abstract：:We have evaluated the ability of immortalized human fibroblasts to recombine transfected plasmid DNA. A number of cell lines from normal individuals and from patients with DNA damage-processing defects were examined. Two plasmid recombination substrates were derived from pSV2neo and contained nonoverlapping deletions ...

journal_title：Plasmid

authors： Timme TL,Wood CM,Moses RE

更新日期：1989-07-01 00:00:00

abstract：:The stable maintenance of certain plasmids in bacterial populations has contributed significantly to the current worldwide antibiotic resistance (AbR) emergency. IncX plasmids, long underestimated in this regard, have achieved recent notoriety for their roles in transmission of resistance to carbapenem and colistin, t...

journal_title：Plasmid

authors： Bustamante P,Iredell JR

更新日期：2017-05-01 00:00:00

abstract：:Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vec...

journal_title：Plasmid

authors： Scott HN,Laible PD,Hanson DK

更新日期：2003-07-01 00:00:00

abstract：:The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the genetically more studied linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the help of an ordered co...

journal_title：Plasmid

authors： Redenbach M,Bibb M,Gust B,Seitz B,Spychaj A

更新日期：1999-11-01 00:00:00

abstract：:A number of plasmid vectors were constructed for high-level gene expression in the dimorphic gram-negative bacterium Caulobacter crescentus. These vectors incorporate the transcription and translation initiation regions of the C. crescentus CB15A rsaA gene, which codes for the abundantly synthesized protein comprising...

journal_title：Plasmid

authors： Bingle WH,Smit J

更新日期：1990-09-01 00:00:00

abstract：:Mobile genetic elements (MGEs) are the genetic material often involved in the interspecies and intraspecies genetic transduction in bacteria. However, little is known about MGEs in the Anginosus group of streptococci (AGS), one of the streptococcal groups found in the oral cavity of humans. We looked for the presence ...

journal_title：Plasmid

authors： Tabata A,Deutsch D,Otsuka S,Verratti K,Tomoyasu T,Nagamune H,Fischetti VA

更新日期：2018-01-01 00:00:00

abstract：:Mobile plasmid-encoded elements are DNA segments that are transferred for horizontal gene transfer and that confer adaptive proprieties, as well as virulence and antibiotic and heavy metal resistance to bacteria. The conjugative plasmid pUM505, isolated from a clinical strain of Pseudomonas aeruginosa, possesses a put...

journal_title：Plasmid

authors： Hernández-Ramírez KC,Reyes-Gallegos RI,Chávez-Jacobo VM,Díaz-Magaña A,Meza-Carmen V,Ramírez-Díaz MI

更新日期：2018-06-01 00:00:00

abstract：:A transient expression system has been developed to compare the relative efficiency of expression of various vaccinia virus DNA sequences containing transcriptional regulatory elements. A plasmid vector was constructed containing both the Escherichia coli galactokinase gene (galK) and the guanine phosphoribosyltransfe...

journal_title：Plasmid

authors： Shepard B,Panicali D,Huang C

更新日期：1987-07-01 00:00:00

abstract：:The effects of the single-strand origins (SSOs) of plasmid pMV158 on (i) the conversion of its single-stranded (ss) replication intermediates to double-stranded (ds) plasmid DNA and (ii) its maintenance were analyzed. The rolling-circle plasmid pMV158, which replicates via ssDNA intermediates, contains two single-stra...

journal_title：Plasmid

authors： Meijer WJ,van der Lelie D,Venema G,Bron S

更新日期：1995-03-01 00:00:00

abstract：:pSAM2 is an 11-kb plasmid integrated in the Streptomyces ambofaciens ATCC23877 and ATCC15154 genomes and found additionally as a free replicon in an uv derivative. After transfer into S. ambofaciens DSM40697 (devoid of pSAM2) or into Streptomyces lividans, specific integration of pSAM2 occurred very efficiently. A 58-...

journal_title：Plasmid

authors： Boccard F,Smokvina T,Pernodet JL,Friedmann A,Guérineau M

更新日期：1989-01-01 00:00:00

abstract：:A novel type I restriction-modification specificity subunit, S. LlaW12I, has been identified on the naturally occurring 8.0-kb plasmid pAW122 in the lactic acid bacterium Lactococcus lactis subsp. cremoris W12. Presence of the HsdS protein together with a complete type I restriction-modification system conferred incre...

journal_title：Plasmid

authors： Madsen A,Westphal C,Josephsen J

更新日期：2000-09-01 00:00:00

abstract：:Transformation of Escherichia coli with Dictyostelium discoideum genomic DNA containing integrated shuttle vectors in multicopy, tandemly duplicated format resulted in the establishment of the linear plasmid molecules as circular monomeric replicons. The transformation efficiencies were comparable to those obtained wi...

journal_title：Plasmid

authors： Barth C,Wilczynska Z,Pontes L,Fraser DJ,Fisher PR

更新日期：1996-09-01 00:00:00

abstract：:A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restrictio...

journal_title：Plasmid

authors： Phillips GJ

更新日期：1999-01-01 00:00:00

abstract：:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...

journal_title：Plasmid

authors： Wabiko H,Kagaya M,Sano H

更新日期：1991-01-01 00:00:00

abstract：:Plasmid deletion formation was compared in wild-type, recE4, and addB72 strains of Bacillus subtilis. Deletion frequencies in plasmid pGP1, as monitored with a penP-lacZ fusion, were low in recE4 and high in addB72, in comparison to the wild-type strain. In the wild-type and recE4 strains, deletions between directly r...

journal_title：Plasmid

authors： Peijnenburg AC,Breed PV,Bron S,Venema G

更新日期：1989-05-01 00:00:00

abstract：:A procedure for the isolation of closed circular mitochondrial DNA (mtDNA) from cells permeabilized with digitonin is described. Compared to the standard procedure in which cells are broken after osmotic swelling, the digitonin-based procedure is more consistent and results in higher mtDNA yields. ...

journal_title：Plasmid

authors： Howell N,Nalty MS,Appel J

更新日期：1986-07-01 00:00:00

abstract：:The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...

journal_title：Plasmid

authors： Osbourn SE,Turner AK,Grinsted J

更新日期：1995-01-01 00:00:00

abstract：:An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...

journal_title：Plasmid

authors： Bradshaw M,Goodnough MC,Johnson EA

更新日期：1998-11-01 00:00:00

abstract：:A circular map of the IncHI plasmid R27 corresponding to a genome size of 182 kb was established using the restriction endonucleases ApaI, XbaI, and PstI. The map was derived from the results obtained by hybridizing individual ApaI and XbaI fragments to blotted digests of the plasmid, as well as from complete and part...

journal_title：Plasmid

authors： Taylor DE,Brose EC

更新日期：1985-01-01 00:00:00

abstract：:Recently an insertional mutagenesis procedure has been developed to permit cloning of genes affected in developmental mutants of Dictyostelium discoideum (Kuspa and Loomis, Proc. Natl. Acad. Sci. USA 89, 8803-8807, 1992). In this procedure a plasmid bearing the URA (pyr5-6) gene is linearized with a restriction enzyme...

journal_title：Plasmid

authors： Chang WT,Gross JD,Newell PC

更新日期：1995-11-01 00:00:00