Abstract:
:In order to study the in vivo assembly of chromatin on prokaryotic DNA templates, we have transformed yeast cells with plasmids pAJ50 and pRB58, which contain pBR322 sequences. In both cases nucleosomes are assembled in vivo on pBR322 DNA, although the nucleosomes are not homogeneous in size. To explore whether there is any preference for nucleosome assembly along pBR322 sequences, we have used an indirect end labeling method. The results indicate that most nucleosomes are placed at random on pBR322, although the probability for histone octamers to interact with some short regions is somewhat reduced. These regions coincide with sequences in which the frequency distribution of nucleosomes reconstituted in vitro (E. Caffarelli et al. (1988) Eur. J. Biochem. 171, 497-501) is low. Sequence determinants that direct chromatin assembly in vitro seem thereby to act to some extent in vivo.
journal_name
Plasmidjournal_title
Plasmidauthors
Estruch F,Pérez-Ortín JE,Matallana E,Franco Ldoi
10.1016/0147-619x(89)90054-1subject
Has Abstractpub_date
1989-03-01 00:00:00pages
113-9issue
2eissn
0147-619Xissn
1095-9890journal_volume
21pub_type
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