Abstract:
:A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.
journal_name
Plasmidjournal_title
Plasmidauthors
Coronado C,Vázquez ME,Cebolla A,Palomares AJdoi
10.1006/plas.1994.1074subject
Has Abstractpub_date
1994-11-01 00:00:00pages
336-41issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(84)71074-2journal_volume
32pub_type
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