Abstract:
:The Tra1 region of RP1 from a derivative with Tn7 inserted into the kanamycin resistance determinant was cloned, using EcoRI, into the multicopy vector plasmid pBR325. For one orientation of the cloned fragment the resultant chimeric plasmid was very frequently lost from the cell, but in the other orientation it was much more stable and also compatible with RP1. Complementation by the stable chimeric plasmid, pED800, of a series of RP1 tra mutants showed that the mutations of all those retaining sensitivity to the P-specific phages PRR1, Pf3, and PR4, or only to PR4, mapped in the Tra1 region, while only 2 out of 20 amber mutations leading to full P-specific phage-resistance did so.
journal_name
Plasmidjournal_title
Plasmidauthors
Watson J,Schmidt L,Willetts Ndoi
10.1016/0147-619x(80)90007-4subject
Has Abstractpub_date
1980-09-01 00:00:00pages
175-83issue
2eissn
0147-619Xissn
1095-9890pii
0147-619X(80)90007-4journal_volume
4pub_type
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