Abstract:
:A small cryptic plasmid designated pHW15 was isolated from Rahnella genomospecies 2 WMR15 and its complete nucleotide sequence was determined. The plasmid contained 3002 bp with a G+C content of 47.4%. The origin of replication was identified by deletion analysis as a region of about 600 bp. This region had an identity of 70% to the replication origin of the ColE1 plasmid at the nucleotide level. Sequence analysis revealed the typical elements: RNA I, RNA II and their corresponding promoters, a sequence allowing hybridisation of RNA II to the DNA and favouring processing by RNaseH, a single-strand initiation determinant (ssi) that allows initiation of lagging-strand synthesis, and a terH sequence required for termination of lagging-strand synthesis. The plasmid contained three expressed open reading frames, one of which showed homology to a ColE1 plasmid-encoded protein. Furthermore, a multimer resolution site was identified by sequence analysis. Its deletion resulted in formation of plasmid multimers during growth leading to an increased plasmid loss rate.
journal_name
Plasmidjournal_title
Plasmidauthors
Rozhon WM,Petutschnig EK,Jonak Cdoi
10.1016/j.plasmid.2006.05.007subject
Has Abstractpub_date
2006-11-01 00:00:00pages
202-15issue
3eissn
0147-619Xissn
1095-9890pii
S0147-619X(06)00058-8journal_volume
56pub_type
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