Abstract：
:Washed cells prepared from carbon-limited continuous cultures of Alcaligenes eutrophus synthesised poly-3-hydroxybutyrate (PHB) rapidly when supplied with glucose, DL-lactate or L-lactate. Unlike growing cultures, washed cells excreted significant amounts of pyruvate. The combined rates of PHB production (qPHB) and pyruvate excretion (qPyr) were linearly related to the rate of carbon substrate utilisation (qS), showing that washed cells behaved similarly to growing cultures when corrected for the absence of non-PHB biomass production. The addition of formate (as a potential source of NADH and/or ATP) significantly stimulated both qPHB and qPyr, but slightly decreased qS and substantially decreased the flux of carbon through the tricarboxylic acid cycle (qTCA). Citrate synthase activity of broken cells was inhibited by physiological concentrations of NADH, but not of ATP, in a manner that was not reversible by AMP. Citrate synthase was purified and shown to be a "large" form of the enzyme (Mr 227,000), comprising a single type of subunit (Mr 47,000) as found in several other gram-negative aerobes. The potential role of citrate synthase in the regulation of PHB production via its ability to control carbon flux into the tricarboxylic acid cycle is discussed.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Henderson RA,Jones CWdoi
10.1007/s002030050526subject
Has Abstractpub_date
1997-12-01 00:00:00pages
486-92issue
6eissn
0302-8933issn
1432-072Xjournal_volume
168pub_type
杂志文章abstract：:Denitrification by Thiobacillus denitrificans "RT" strain was investigated using manometry and gas chromatography. 1. From nitrate, resting cells produced only nitrogen anaerobically with thiosulfate as the electron donor. The data suggest that nitrate was assimilated and dissimilated by the same nitrate reductase, as...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436326
更新日期：1975-03-12 00:00:00
abstract：:Pure cultures of an aerobically grown Flavobacterium sp. were shown by hydride generation-cold trap-atomic absorption spectrometry to biomethylate inorganic antimony (III) supplied as potassium antimony tartrate. Growth inhibition of the Flavobacterium sp. by antimony (III) over the range 0-30 mg Sb l(-1) was assessed...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0456-9
更新日期：2002-10-01 00:00:00
abstract：:An aerobic, Gram-negative, motile, rod-shaped bacterium designated KMM 9500(T) was isolated from a sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis affiliated strain KMM 9500(T) to the genus Pseudomonas as a distinct subline clustered with Pseudomonas marincola KMM ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1103-6
更新日期：2015-06-01 00:00:00
abstract：:The most important tools in killing and overcoming on the microbes and pathogens that cause diseases in medicine and/or in agriculture are the antibiotics. The discovery and synthesis of the microbial natural products or antibiotics has greatly developed genetically and biotechnologically quickly in the last decades. ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02085-6
更新日期：2020-10-19 00:00:00
abstract：:In the Tat protein export pathway of Gram-negative bacteria, TatA and TatB are homologous proteins that carry out distinct and essential functions in separate sub-complexes. In contrast, Gram-positive Tat systems usually lack TatB and the TatA protein is bifunctional. We have used a mutagenesis approach to delineate T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-011-0699-4
更新日期：2011-08-01 00:00:00
abstract：:Plasmid-borne hup-lacZ transcriptional fusion constructs were introduced into three separate mutant strains of Bradyrhizobium japonicum which express hydrogenase constitutively (Hupc strains SR470, SR473 and JH101) in both autotrophic and heterotrophic environments. The lacZ structural gene linked directly to the regu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00258144
更新日期：1993-01-01 00:00:00
abstract：:Many bacterial cells are known to regulate their cooperative behaviors and physiological processes through a molecular mechanism called quorum sensing. Quorum sensing in Serratia marcescens JG is mediated by the synthesis of autoinducer 2 (AI-2) which is a furanosyl borate diester. In this study, the response of quoru...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-016-1213-9
更新日期：2016-08-01 00:00:00
abstract：:AMP-degrading pathways in Azotobacter vinelandii cells were investigated. AMP nucleosidase (EC 3.2.2.4) was rapidly synthesized and reached a maximum at 24 h, while the activity of 5'-nucleotidase (EC 3.1.3.5) specific for AMP, which was negligible during the logarithmic phase of the growth, first appeared in 24 h-cul...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406162
更新日期：1980-12-01 00:00:00
abstract：:Cyanothece sp. strain ATCC 51142 is an aerobic, unicellular, diazotrophic cyanobacterium that temporally separates O2-sensitive N2 fixation from oxygenic photosynthesis. The energy and reducing power needed for N2 fixation appears to be generated by an active respiratory apparatus that utilizes the contents of large i...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:The natural role of antibiotics in the ecology of Streptomyces is debated and still largely unknown. The predatory myxobacteria and many other genera of prokaryotic epibiotic and wolfpack predators across different taxa possess secondary metabolites with antimicrobial action, and these compounds have a role in predati...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-0961-7
更新日期：2014-04-01 00:00:00
abstract：:Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0848-4
更新日期：2013-01-01 00:00:00
abstract：:VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1069-9
更新日期：2015-04-01 00:00:00
abstract：:Chlorobenzoates (CBA) arise as intermediates during the degradation of polychlorinated biphenyls (PCBs) and some chlorinated herbicides. Since PCBs were produced as complex mixtures, a range of mono-, di-, and possibly trichloro-substituted benzoates would be formed. Chlorobenzoate degradation has been proposed to be ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF01692864
更新日期：1996-03-01 00:00:00
abstract：:Recently, we reported the synthesis of five bacteriocin-like inhibitor substances (Bt-BLIS: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by Mexican strains of Bacillus thuringiensis. Here we show that, collectively, these Bt-BLIS have a moderate to broad spectrum of antibacterial acti...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0414-2
更新日期：2008-12-01 00:00:00
abstract：:Mannoproteins from cell walls of Saccharomyces cerevisiae synthesized at successive stages of the population growth cycle have been solubilized with Zymolyase and subsequently analyzed. The major change along the population cycle concerned a large size mannoprotein material; the size of the newly-synthesized molecules...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425354
更新日期：1987-07-01 00:00:00
abstract：:The distribution of sex pheromone induced aggregation substance was studied on the cell surface of various Enterococcus faecalis strains. In the accompanying paper we have shown that the aggregation substance appears as a layer of hairlike structures. Using direct and indirect immunogold technique, transmission electr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454864
更新日期：1989-01-01 00:00:00
abstract：:During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed functi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002039900131
更新日期：2000-04-01 00:00:00
abstract：:In yeasts, several sensing systems localized to the plasma membrane which transduce information regarding the availability and quality of nitrogen and carbon sources and work in parallel with the intracellular nutrient-sensing systems, regulate the expression and activity of proteins involved in nutrient uptake and ut...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0022-3
更新日期：2005-11-01 00:00:00
abstract：:Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00444661
更新日期：1989-01-01 00:00:00
abstract：:Acinetobacter calcoaceticus LMD 79.41 produced significant amounts of pyrrolo-quinoline quinone (PQQ) in its culture medium when grown on quinic acid or shikimic acid. Studies with LMD 79.41 and PQQ- -mutants of this strain demonstrated that this organism contains an NAD(P)-independent quinate dehydrogenase (QDH) (EC ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409714
更新日期：1988-05-01 00:00:00
abstract：:Gas chromatography-mass spectrometry studies of the nitrogen isotopic composition of the N-trifluoroacetyl n-butyl ester derivatives of the amino acids from isolated hydrolyzed cyanophycin from 15N-enriched cells led to two major findings: (1) the amino acid composition of this granular polypeptide, isolated using pro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00314469
更新日期：1994-01-01 00:00:00
abstract：:Studying the effects of microbial volatile organic compounds (VOCs) on plant growth is challenging because the production of volatiles depends on many environmental factors. Adding to this complexity, the method of volatile exposure itself can lead to different responses in plants and may account for some of the contr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1104-5
更新日期：2015-06-01 00:00:00
abstract：:Two bacterial isolates from Great Bay Estuary, New Hampshire, in co-culture carried out anaerobic dissimilation of citric acid with Fe(III) as the terminal electron acceptor. Neither isolate oxidized citrate with Fe(III) anaerobically in axenic culture. The Fe(III) reducer, Shewanella alga strain BrY, did not grow ana...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050383
更新日期：1996-10-17 00:00:00
abstract：:L-(+)-lactate dehydrogenase (LDH) from Staphylococcus epidermidis ATCC 14990 was purified by affinity chromatography. The purified enzyme was specifically activated by fructose-1,6-diphosphate (FDP). The concentration of FDP required for 50% maximal activity was about 0.15 mM. The enzyme activity was inhibited by aden...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447150
更新日期：1975-11-07 00:00:00
abstract：:The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was de...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0533-4
更新日期：2010-02-01 00:00:00
abstract：:Microbial changes in vaginal ecosystem may accelerate the process of cervical carcinogenesis. The developed cervical cancer can lead to changes in the vaginal microbiota. The aim of our study is to determine the vaginal microbiota changes at women with FIGO I stage cervical cancer. We conducted an open, single-site su...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01747-4
更新日期：2020-03-01 00:00:00
abstract：:Edwardsiella tarda (E. tarda) is distributed widely in a variety of hosts including humans, other mammals and fish, and it is worthwhile to notice that E. tarda -caused fish infections lead to the most important bacterial disease in fish. Considering Eha acting as a transcriptional regulator in E. tarda strain ET13 ha...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01794-x
更新日期：2020-05-01 00:00:00
abstract：:1. The daily progress of NADP-linked GPD-activity rise in synchronous culture of Ankistrodesmus braunii was investigated in respect to short time increase of activity by light. After various exposure times cells were temporarily deprived of light and subsequently the so-called dark value as well as the light value (da...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：
更新日期：1975-06-22 00:00:00
abstract：:Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01618-y
更新日期：2019-03-01 00:00:00
abstract：:The mechanism of the massive extracellular production of citric and isocitric acids by Saccharomycopsis lipolytica grown on n-paraffins has been studied. When growth stops, because of nitrogen limitation, the intracellular concentration of ATP sharply rises whereas that of AMP and ADP decreases to a low level. At the ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428588
更新日期：1977-05-13 00:00:00
