Abstract：
:VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibodies confirmed that the secretion of at least four flagellar components, such as FlaA, FlgL, FlgE, and FlgM, was affected by the vcrD1 mutation, which was consistent with decreased motility on soft agar plates and the non-flagellated morphology of the mutant. The ΔexsA mutant, another T3SS1 mutant, did not showed reduced motility, but became non-motile phenotype with the additional ΔvcrD1 mutation. Complementation of wild-type vcrD1 gene into ΔvcrD1 mutant resulted in restored motility. Fractionation of bacterial cytoplasm from the periplasm and membrane revealed lower levels of FlaA and FlgM in the cytoplasm of the ΔvcrD1 mutant, indicating that VcrD1 might regulate the expression of flagellar genes in addition to the secretion of flagellar components in V. parahaemolyticus. Quantitative RT-PCR assays of seven representative flagellar genes in the wild-type and ΔvcrD1 mutant strains demonstrated that transcript levels of two early flagellar genes, flaK and flaL, were not reduced by the vcrD1 mutation, whereas the middle and late flagellar genes were expressed at a lower level in the vcrD1 mutant. This study raises a possibility that VcrD1 plays a role in flagellar morphogenesis in V. parahaemolyticus by regulating the expression and secretion of flagellar components.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Noh HJ,Nagami S,Kim MJ,Kim J,Lee NK,Lee KH,Park SJdoi
10.1007/s00203-014-1069-9subject
Has Abstractpub_date
2015-04-01 00:00:00pages
397-410issue
3eissn
0302-8933issn
1432-072Xjournal_volume
197pub_type
杂志文章abstract：:An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China. The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods. The strain was aerobic with optimal gro...
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-04-01 00:00:00
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