Probiotic properties of Oxalobacter formigenes: an in vitro examination.

Abstract:

:Oxalobacter formigenes (O. formigenes) is a nonpathogenic, Gram-negative, obligate anaerobic bacterium that commonly inhabits the human gut and degrades oxalate as its major energy and carbon source. Results from a case-controlled study suggested that lack of O. formigenes colonization is a risk factor for recurrent calcium oxalate stone formation. Hence, O. formigenes colonization may prove to be an efficacious method for limiting calcium oxalate stone risk. However, challenges exist in the preparation of O. formigenes as a successful probiotic due to it being an anaerobe with fastidious growth requirements. Here we examine in vitro properties expected of a successful probiotic strain. The data show that the Group 1 O. formigenes strain OxCC13 is sensitive to pH < 5.0, persists in the absence of oxalate, is aerotolerant, and survives for long periods when freeze-dried or mixed with yogurt. These findings highlight the resilience of this O. formigenes strain to some processes and conditions associated with the manufacture, storage and distribution of probiotic strains.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Ellis ML,Dowell AE,Li X,Knight J

doi

10.1007/s00203-016-1272-y

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

1019-1026

issue

10

eissn

0302-8933

issn

1432-072X

pii

10.1007/s00203-016-1272-y

journal_volume

198

pub_type

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