Abstract：
:YfkE, a protein from Bacillus subtilis, exhibits homology to the Ca(2+):Cation Antiporter (CaCA) Family. In a fluorescence-based assay of everted membrane vesicles prepared from Na(+)(Ca(2+))/H(+) antiporter-defective mutant Escherichia coli KNabc, YfkE exhibited robust Ca(2+)/H(+) antiport activity, with a K (m) for Ca(2+) estimated at 12.5 muM at pH 8.5 and 113 muM at pH 7.5. Neither Na(+) nor K(+) served as a substrate. Mg(2+) also did not serve as a substrate, but inhibited the Ca(2+)/H(+) antiporter activity. The Ca(2+) transport capability of YfkE was also observed directly by transport assays in everted membrane vesicles using radiolabeled (45)Ca(2+). Transcriptional analysis from the putative yfkED operon using beta-garactosidase activity as a reporter revealed that both of the yfkE and yfkD genes are regulated by forespore-specific sigma factor, SigG, and the general stress response regulator, SigB. These results suggest that YfkE may be needed for Ca(2+) signaling in the sporulation or germination process in B. subtilis. ChaA is proposed as the designation for YfkE of B. subtilis.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Fujisawa M,Wada Y,Tsuchiya T,Ito Mdoi
10.1007/s00203-009-0494-7subject
Has Abstractpub_date
2009-08-01 00:00:00pages
649-57issue
8eissn
0302-8933issn
1432-072Xjournal_volume
191pub_type
杂志文章abstract：:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...
journal_title：Archives of microbiology
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journal_title：Archives of microbiology
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doi：10.1007/s00203-007-0265-2
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doi：10.1007/BF00425346
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-018-1602-3
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01789-8
更新日期：2020-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-020-02073-w
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pub_type： 杂志文章
doi：10.1007/BF00409846
更新日期：1983-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-003-0554-3
更新日期：2003-06-01 00:00:00
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更新日期：2018-03-01 00:00:00
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doi：10.1007/s00203-009-0535-2
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pub_type： 杂志文章
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更新日期：1989-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章,评审
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更新日期：2011-02-01 00:00:00
