Influence of sugar source (lactose, glucose, galactose) on 2,3-butanediol production by Klebsiella oxytoca NRRL-B199.

Abstract:

:The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentation broths with lactose, while it was the main product from glucose + galactose with final concentrations of up to 18.8g/l in media at pH 6.0. Under conditions optimal for 2,3-butanediol synthesis, when aeration limited growth, the rate of biomass growth was more tightly related to the aeration rate in lactose medium than in glucose + galactose medium. These relations suggest that the growth rate is very low on lactose but still considerable on glucose + galactose when aeration rate tends toward zero. Corresponding, the metabolism is more oxidative in the former medium, yielding mainly acetate as product.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Champluvier B,Decallonne J,Rouxhet PG

doi

10.1007/BF00446920

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

411-4

issue

5

eissn

0302-8933

issn

1432-072X

journal_volume

152

pub_type

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