Abstract：
:Pseudomonas cepacia grew rapidly using a mixture of all three branched chain amino acids as carbon source, but failed to use individual branched chain amino acids as sole carbon source. Extracts of bacteria grown on branched chain amino acids had between 2- and 3-fold higher levels of alpha-ketoglutarate-dependent branched chain amino acid aminotransferase activity than extracts of glucose-grown bacteria. The increase in enzyme activity was due to the presence of a second aminotransferase not detected in extracts of glucose-grown bacteria. The enzyme, which presumably plays a role in branched chain amino acid degradation, had an apparent molecular weight (mol.wt.) of 75,000. The other aminotransferase was formed constitutively and apparently functions in synthesis of branched chain amino acids. It was more stable than the 75,000 mol.wt. enzyme, and was purified to homogeneity and found to be a 180,000 mol.wt. oligomer containing 6 subunits of approximately 30,000 mol.wt. Antiserum prepared against the purified enzyme inhibited its activity but failed to influence the activity of the 75,000 mol.wt. aminotransferase, suggesting that the two isoenzymes are encoded by different genes.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Wong HC,Lessie TGdoi
10.1007/BF00423069subject
Has Abstractpub_date
1979-03-12 00:00:00pages
223-9issue
3eissn
0302-8933issn
1432-072Xjournal_volume
120pub_type
杂志文章abstract：:A triazine dye, Cibacron blue 3G-A (CB), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of CB on growth of gram-positive bacteria other than Staphylococcus. CB added to the medium of growing cultures of strains of genus Micrococcus, Streptococcus, Lactobacillus and Bacillus...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00248617
更新日期：1991-01-01 00:00:00
abstract：:A thermostable homodimeric isocitrate dehydrogenase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus was purified and characterized. The mol. mass of the isocitrate dehydrogenase subunit was 42 kDa as determined by SDS-PAGE. Following separation by SDS-PAGE, A. fulgidus isocitrate dehydrogen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050516
更新日期：1997-11-01 00:00:00
abstract：:Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced beta-oxidation pathway showed the presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase. After isopicnic centrifugation in a ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050392
更新日期：1996-11-01 00:00:00
abstract：:Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach. The optimum growth temperature was 37 °C and the optimum pH was 7.5-...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02003-w
更新日期：2021-01-01 00:00:00
abstract：:Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402302
更新日期：1978-05-30 00:00:00
abstract：:We previously constructed an arabinose-inducible recombinant Bifidobacterium longum that could efficiently express secreted IFN-alpha2b in vitro (Deng et al. in Arch Microbiol 191:681-686, 2009). Here, we investigated the influence of oral pBAD-SPIFN-transformed B. longum on immunomodulation and IFN-induced Mx1 gene t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0589-1
更新日期：2010-08-01 00:00:00
abstract：:The Gram-positive bacterium Clavibacter michiganensis subsp. sepedonicus is the causal agent of bacterial wilt and ring rot of potato. So far, only two proteins have been shown to be essential for virulence, namely a plasmid-encoded cellulase CelA and a hypersensitive response-inducing protein. We have examined the re...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0301-2
更新日期：2008-02-01 00:00:00
abstract：:Bacillus thuringiensis (Bt) is efficient, strongly specific, and avirulent to humans, making it one of the most popular biopesticides in the world. Bt LLP29 is a mosquitocidal strain that was first isolated from Magnolia denudata. To understand its molecular mechanism against mosquitoes, the genome of Bt LLP29 was seq...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01875-2
更新日期：2020-09-01 00:00:00
abstract：:Glutamine synthetase (GS) activity of Schizosaccharomyces pombe 972 was high in ammonia-limited cultures, low in phosphate- and sulphate-limited cultures and not detected in glucose-limited cultures. When ammonia was 'pulsed' into an ammonia-limited culture then GS activity decreased at a rate faster than that calcula...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00549365
更新日期：1977-01-11 00:00:00
abstract：:The isolated cell wall of Streptomyces griseus 52-1 strain labelled with fluorescein isothiocyanate (FITC) and containing wall-bound autolytic enzyme was lysed as a function of different cations. The autolysis was accelerated by aminoglycoside antibiotics (streptomycin and the structurally closely related neomycin) wh...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00291282
更新日期：1990-01-01 00:00:00
abstract：:We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0339-1
更新日期：2008-05-01 00:00:00
abstract：:Studies are presented of the biliproteins of Anabaena sp. This filamentous cyanobacterium contains three major biliproteins. Whereas two of these, C-phycocyanin and allophycocyanin, are common to all cyanobacteria, the third, phycoerythrocyanin (gammamax approximately 568 nm) has hitherto not been described and its di...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00416970
更新日期：1976-10-11 00:00:00
abstract：:During a screening program intended to identify genes encoding enzymes typical for secondary metabolism in Sorangium cellulosum So ce90, an aromatic amino acid decarboxylase gene (ddc) was detected. Expression of ddc in Escherichia coli and subsequent enzyme assays with cell-free extracts confirmed the proposed functi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002039900131
更新日期：2000-04-01 00:00:00
abstract：:The control of pyrimidine nucleotide formation in the bacterium Pseudomonas aurantiaca ATCC 33663 by pyrimidines was studied. The activities of the pyrimidine biosynthetic pathway enzymes were investigated in P. aurantiaca ATCC 33663 cells and from cells of an auxotroph lacking orotate phosphoribosyltransferase activi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01842-x
更新日期：2020-08-01 00:00:00
abstract：:The molar percentages of guanine plus cytosine in the DNA of 51 strains of Simonsiellaceae were determined by buoyant density ultracentrifugation of cell lysates in CsCl. The DNA base ratios ranged from 41-55 mole-% guanine plus cytosine. These values fall within the range known for the Order Cytophagales, the non-fru...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492026
更新日期：1977-06-20 00:00:00
abstract：:The role of the genomic bipyrimidine nucleotide frequency in pyrimidine dimer formation caused by germicidal UV radiation was studied in three microbial reference organisms (Escherichia coli K12, Deinococcus radiodurans R1, spores and cells of Bacillus subtilis 168). The sensitive HPLC tandem mass spectrometry assay w...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0579-3
更新日期：2010-07-01 00:00:00
abstract：:Escherichia coli, Salmonella enterica, Klebsiella pneumoniaeand Klebsiella oxytocawere found to contain two D-tagatose 1,6-bisphosphate (TagBP)-specific aldolases involved in catabolism of galactitol (genes gatY gatZ) and of N-acetyl-galactosamine and D-galactosamine (genes kbaY kbaZ,also called agaY agaZ). The two al...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0406-6
更新日期：2002-05-01 00:00:00
abstract：:Endophytic bacteria have been reported to have symbiotic, mutualistic, commensalistic or trophobiotic relationships with various plant parts. As part of its adaptation, many endophytic organisms are known to exhibit properties with multiple beneficial effects to the plant system. Even though many bacterial genera have...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01911-1
更新日期：2020-09-01 00:00:00
abstract：:The synthesis of RNA was studied during the synchronous germination of Blastocladia ramosa zoospores. Comparison of RNA synthesis during germination of B. ramosa and Blastocladiella emersonii zoospores revealed that B. ramosa has a longer lag time before RNA synthesis is initiated and, in addition, the rate of RNA syn...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00446529
更新日期：1975-12-31 00:00:00
abstract：:Xanthobacter tagetidis grew as a chemolithotrophic autotroph on thiosulfate and other inorganic sulfur compounds, as a heterotroph on thiophene-2-carboxylic acid, acetic acid and alpha-ketoglutaric acid, and as a mixotroph on thiosulfate in combination with thiophene-2-carboxylic acid and/or acetic acid. Autotrophic g...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050568
更新日期：1998-03-01 00:00:00
abstract：:Pulicat Lake sediments are often severely polluted with the toxic heavy metal mercury. Several mercury-resistant strains of Bacillus species were isolated from the sediments and all the isolates exhibited broad spectrum resistance (resistance to both organic and inorganic mercuric compounds). Plasmid curing assay show...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0088-6
更新日期：2006-04-01 00:00:00
abstract：:Colistin is a mixture of polymyxin E1 and E2, bactericidal pentacationic lipopeptides used to treat infections caused by Gram-negative pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae. Industrial production of colistin is obtained by a fermentation process of the natural producer Paenibacillus polymy...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1084-5
更新日期：2015-05-01 00:00:00
abstract：:Saccharomyces cerevisiaepresents two alternative vegetative forms of growth, switching between yeast forms to pseudohyphal forms depending on the specific environmental conditions. To identify genes involved in cell wall morphogenesis, a haploid S. cerevisiae monomorphic mutant, W27, which exhibits pseudohyphal growth...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0400-z
更新日期：2002-04-01 00:00:00
abstract：:Serine (Ser)/threonine (Thr) or tyrosine (Tyr) protein kinases in eukaryotes contain RDxKxxN or RDx(A/R)A(A/R)N sequences, respectively, in the catalytic loop. Myxococcus xanthus DspB is a dual-specificity kinase that contains an atypical sequence, RDVAQKN, in the catalytic loop. The DspB mutant (A165K), which contain...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1181-5
更新日期：2016-04-01 00:00:00
abstract：:A highly efficient method of transposon mutagenesis was developed for genetic analysis of Xanthobacter autotrophicus Py2. The method makes use of a transposon delivery vector that encodes a hyperactive Tn 5 transposase that is 1,000-fold more active than the wild-type transposase. In this construct, the transposase is...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0442-2
更新日期：2002-09-01 00:00:00
abstract：:The signal orchestration between legumes and the rhizobia attribute to symbiotic nitrogen fixation through nodule formation. Root nodules serve as a nutrient-rich reservoir and harbor diverse microbial communities. However, the existence of non-rhizobial endophytes (NRE) and their role inside the root nodules are bein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01983-z
更新日期：2020-12-01 00:00:00
abstract：:Anaerobic digestion, a recently hot technology to produce biogases especially methane generation for biofuel from wastewater, is considered an effective explanation for energy crisis and global pollution threat. A complex microbiome population is present in sludge, which plays an important role in the digestion of com...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01716-x
更新日期：2020-01-01 00:00:00
abstract：:The thermotolerant methylotroph Bacillus sp. C1 possesses a novel NAD-dependent methanol dehydrogenase (MDH), with distinct structural and mechanistic properties. During growth on methanol and ethanol, MDH was responsible for the oxidation of both these substrates. MDH activity in cells grown on methanol or glucose wa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245161
更新日期：1992-01-01 00:00:00
abstract：:The family Chlamydiaceae contains nine species pathogenic to humans and animals, but their routine identification is hampered by inadequate detection methods. In an attempt to find a new region for PCR detection and discrimination of the Chlamydiaceae species, the 3' end of the omp2 gene of Chlamydiaceae has been exam...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0757-2
更新日期：2005-03-01 00:00:00
abstract：:Acinetobacter species remain alive in hospitals on various surfaces, both dry and moist, forming an important source of hospital infections. These bacteria are naturally resistant to many antibiotic classes. Although the role of the quorum sensing system in regulating the virulence factors of Acinetobacter species has...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1408-8
更新日期：2017-12-01 00:00:00
