Abstract：
:The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermophila, two thermophilic spirochetes, Treponema bryantii, T. denticola, paragraph signT. pectinovorum, Leptospira biflexa and L. interrogans. All of the Spirochaeta strains, regardless of their phenotype, possessed primarily a pyrophosphate-dependent phosphofructokinase. In contrast, T. bryantii, T. denticola and L. biflexa had predominantly an ATP-dependent activity, whereas no activity was detected in T. pectinovorum or paragraph signL. interrogans. The results suggest that pyrophosphate-dependent phosphofructokinase activity may be a reliable phenotypic marker for the genus Spirochaeta and that there are potentially interesting differences in how the catabolism of saccharides is controlled among members of genera within the Spirochaetales. The pyrophosphate-dependent phosphofructokinase from S. thermophila strain RI 19.B1 was purified (303-fold) to homogeneity and biochemically characterised. The S. thermophila enzyme displayed hyperbolic kinetics with respect to both the forward and reverse cosubstrates and was not significantly affected by traditional activators or inhibitors of phosphofructokinase. The biochemical characterisation represents the first spirochete phosphofructokinase to be described.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Ronimus RS,Morgan HW,Ding YRdoi
10.1007/s002030050777keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
401-6issue
6eissn
0302-8933issn
1432-072Xpii
91720401.203journal_volume
172pub_type
杂志文章abstract：:A novel coccoid-shaped, hyperthermophilic, heterotrophic member of the archaea was isolated from a shallow marine hydrothermal system at Vulcano Island, Italy. The isolate grew between 56 and 90 degrees C with an optimum around 85 degrees C. The pH range for growth was 6.5 to 10.5, with an optimum around 9.0. Polysulf...
journal_title：Archives of microbiology
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journal_title：Archives of microbiology
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doi：10.1007/BF00421893
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journal_title：Archives of microbiology
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journal_title：Archives of microbiology
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doi：10.1007/BF00314469
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doi：10.1007/BF00403209
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journal_title：Archives of microbiology
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doi：10.1007/s00203-014-0992-0
更新日期：2014-08-01 00:00:00
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doi：10.1007/BF00446749
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428588
更新日期：1977-05-13 00:00:00
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journal_title：Archives of microbiology
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doi：10.1007/BF00446549
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journal_title：Archives of microbiology
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doi：10.1007/BF00415280
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050465
更新日期：1997-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-006-0095-7
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00444661
更新日期：1989-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-012-0808-z
更新日期：2012-09-01 00:00:00
abstract：:The radiation resistant bacteria Micrococcus radiophilus and M. radioproteolyticus were studied by thin sectioning and freez-etching techniques and the two species were found to be similar in the fine structure. The only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425346
更新日期：1976-04-01 00:00:00
