Abstract：
:The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotrophs (type I and type II MOB, respectively) as well as methanotrophs from the phylum Verrucomicrobia. We updated and established the weighted mean pmoA gene cutoff values on the nucleotide level at 86, 82, and 71 % corresponding to the 97, 95, and 90 % similarity of the 16S rRNA gene. Based on these cutoffs, the functional gene fragments can be entirely processed at the nucleotide level throughout software platforms such as Mothur or QIIME which provide a user-friendly and command-based alternative to amino acid-based pipelines. Type II methanotrophs are less divergent than type I both with regard to ribosomal and functional gene sequence similarity and GC content. We suggest that this agrees with the theory of different life strategies proposed for type I and type II MOB.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Wen X,Yang S,Liebner Sdoi
10.1007/s00203-016-1222-8subject
Has Abstractpub_date
2016-09-01 00:00:00pages
629-36issue
7eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-016-1222-8journal_volume
198pub_type
杂志文章abstract：:The ADPglucose pyrophosphorylases from Rhodopseudomonas sphaeroides and Rhodopseudomonas gelatinosa are activated by fructose-6-phosphate, pyruvate and fructose-1,6 biophosphate-P2. The effects of the activators are to increase significantly the Vmax of ADPglucose synthesis and to lower the S0.5 values (concentration ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421887
更新日期：1980-05-01 00:00:00
abstract：:Mycothiol (MSH) plays important roles in maintaining cytosolic redox homeostasis and in adapting to reactive oxygen species in the high-(G + C)-content Gram-positive Actinobacteria. However, its physiological roles are ill defined compared to glutathione, the functional analog of MSH in Gram-negative bacteria and most...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0889-3
更新日期：2013-06-01 00:00:00
abstract：:An anaerobic, mesophilic, syntrophic benzoate-degrading bacterium, designated strain FB(T), was isolated from methanogenic sludge which had been used to treat wastewater from the manufacture of terephthalic acid. Cells were non-motile gram-positive rods that formed spores. The optimum temperature for growth was 35-40 ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-003-0521-z
更新日期：2003-04-01 00:00:00
abstract：:The bacteriocinogenic strain of Enterococcus faecalis EJ97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050678
更新日期：1998-12-01 00:00:00
abstract：:Video-enhanced light microscopy of the apical and subapical regions of growing hyphae of several fungal species revealed the existence of momentary synchronized motions of subcellular organelles. First discovered in a temperature-sensitive morphological mutant (ramosa-1) of Aspergillus niger, these seemingly spontaneo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0771-z
更新日期：2005-05-01 00:00:00
abstract：:The diversity of eukaryotic populations, in particular protozoa, in the water supplies of intensively reared broilers has not been previously studied. This important food-rearing environment was screened for the molecular diversity of eukaryotes by the analysis of PCR-amplified 18S rRNA. DNA was extracted from filtere...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-006-0119-3
更新日期：2006-07-01 00:00:00
abstract：:Differences in the rumen bacterial community have been previously reported for Soay sheep housed under different day length conditions. This study extends this previous investigation to other organs of the digestive tract, as well as the analysis of ciliated protozoa and anaerobic fungi. The detectable concentrations ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01652-w
更新日期：2019-09-01 00:00:00
abstract：:The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0276-z
更新日期：2007-12-01 00:00:00
abstract：:The cold shock response in bacteria involves the expression of low-molecular weight cold shock proteins (CSPs) containing a nucleic acid-binding cold shock domain (CSD), which are known to destabilize secondary structures on mRNAs, facilitating translation at low temperatures. Caulobacter crescentus cspA and cspB are ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0602-8
更新日期：2010-09-01 00:00:00
abstract：:The haloalkaliphilic archaeon Natronobacterium vacuolatum forms cylinder-shaped gas vesicles throughout the growth cycle when grown in media containing 15-25% NaCl. Cells cultivated in media containing 13% NaCl are, however, gas-vesicle-free. The major gas vesicle structural protein, nv-GvpA, was detected by an antise...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050465
更新日期：1997-07-01 00:00:00
abstract：:A strain of Pseudomonas fluorescens was capable of co-metabolizing 3-chlorobenzoic acid with the production of a chlorinated catechol black pigment. A peroxidase and another enzymatic activity referred to as a polyphenol oxidase were found to be involved in the oxidation of 4-chlorocatechol to 4-chloro-1,2-benzoquinon...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00252220
更新日期：1993-01-01 00:00:00
abstract：:Many pathogenic bacteria express filamentous appendages, termed pili, on their surface. These organelles function in several important bacterial processes, including mediating bacterial interaction with, and colonization of the host, signalling events, locomotion, DNA uptake, electric conductance, and biofilm formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-015-1117-0
更新日期：2015-08-01 00:00:00
abstract：:The presence of one periplasmic [NiFe] hydrogenase, one periplasmic [Fe] hydrogenase, and one cytoplasmic NADP-reducing hydrogenase has been previously established in Desulfovibrio fructosovorans. In the present work, marker-exchange mutagenesis was performed to determine the function of the tetrameric NADP-reducing h...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050414
更新日期：1997-01-01 00:00:00
abstract：:The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 micro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413136
更新日期：1989-01-01 00:00:00
abstract：:The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050505
更新日期：1997-10-01 00:00:00
abstract：:The radiation resistant bacteria Micrococcus radiophilus and M. radioproteolyticus were studied by thin sectioning and freez-etching techniques and the two species were found to be similar in the fine structure. The only significant difference was in the appearance of the surfaces of the cell walls in freeze-etched pr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425346
更新日期：1976-04-01 00:00:00
abstract：:The signal orchestration between legumes and the rhizobia attribute to symbiotic nitrogen fixation through nodule formation. Root nodules serve as a nutrient-rich reservoir and harbor diverse microbial communities. However, the existence of non-rhizobial endophytes (NRE) and their role inside the root nodules are bein...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01983-z
更新日期：2020-12-01 00:00:00
abstract：:Carotenogenic mutants of Corynebacterium glutamicum were analyzed for their carotenoid content. Mutant MV10 accumulated the same carotenoids as the wild-type, decaprenoxanthin, decaprenoxanthin monoglucoside, and (2R,6R,2'R,6'R)-decaprenoxanthin di-(beta-D)-glucoside, but in three-fold higher amounts. In addition, dec...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030100315
更新日期：2001-09-01 00:00:00
abstract：:The nitrogen-fixing bacterial strain UFLA 01-1174T was isolated from nodules of Campsiandra laurilifolia Benth. originating from the Amazon region, Brazil. Its taxonomic position was defined using a polyphasic approach. Analysis of the 16S rRNA gene placed the strain in the Bradyrhizobium genus, the closest species be...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02022-7
更新日期：2021-01-01 00:00:00
abstract：:To analyze if chemical cell wall alterations contribute to penicillin-induced bacteriolysis, changes in the amount, stability, and chemical composition of staphylococcal cell walls were investigated. All analyses were performed before onset of bacteriolysis i.e. during the first 60 min following addition of different ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00249181
更新日期：1990-01-01 00:00:00
abstract：:Acanthamoeba castellanii is a free-living amoeba found mainly in humid environments and Arcobacter butzleri is an emerging zoonotic pathogen, both can establish in vitro endosymbiotic relationships in the absence of bacterial replication. We analyzed the localization of A. butzleri within A. castellanii establishing t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01699-9
更新日期：2019-12-01 00:00:00
abstract：:In this study, chromium (Cr)-tolerant bacteria were test for their efficiency in alleviating Cr stress in Cicer arietinum plants. On the basis of 16S rRNA gene analysis, the isolates were identified belonging to genus Stenotrophomonas maltophilia, Bacillus thuringiensis B. cereus, and B. subtilis. The strains produced...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01801-1
更新日期：2020-05-01 00:00:00
abstract：:The Roseobacter clade, belonging to the family Rhodobacteraceae of the class Alphaproteobacteria, is one of the major bacterial groups in marine environments. A remarkable wealth of diverse large plasmids has been detected in members of this lineage. Here, we analysed the genome structure and extrachromosomal DNA cont...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0535-2
更新日期：2010-02-01 00:00:00
abstract：:Some unicellular N(2)-fixing cyanobacteria have recently been found to lack a functional photosystem II of photosynthesis. Such organisms, provisionally termed UCYN-A, of the oceanic picoplanktion are major contributors to the global marine N-input by N(2)-fixation. Since their photosystem II is inactive, they can per...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-010-0621-5
更新日期：2010-10-01 00:00:00
abstract：:The fine structure of the Gram-negative filamentous gliding bacterium, Herpetosiphon is described. The outer membrane of the cell envelope could not be resolved as a separate structure, probably because it is fused with the underlying dense (peptidoglycan) layer. There was an additional wall layer outside this membran...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00428379
更新日期：1975-03-10 00:00:00
abstract：:The growth, duplication and fate of multikaryotic hyphae bearing true clamp connections, as derived from compatible matings of Schizophyllum commune, were studied by phase contrast microscopy. The nuclei (N) of multikaryotic apices maintained a near central position during hyphal growth. True clamp connection formatio...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406155
更新日期：1980-12-01 00:00:00
abstract：:The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH4+ intracellularly. When growing on nitrogen sources other than NH4+, the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402008
更新日期：1984-10-01 00:00:00
abstract：:The ability of Methylosinus trichosporium OB3b, expressing soluble methane monooxygenase, to oxidize a range of ortho-substituted biphenyls was examined to better understand how substituents affect both the rate and products of oxidation in comparison to biphenyl. Inhibition of oxidation was observed over the tested s...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030000170
更新日期：2000-07-01 00:00:00
abstract：:The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406170
更新日期：1980-12-01 00:00:00
abstract：:A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245249
更新日期：1992-01-01 00:00:00
