Abstract:
:The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 microM). The maximal uptake rates for all three substrates were very similar (0.94 - 1.30 nmol/mg dw.min). The optimum of amino acid uptake was at pH 8.5 and the activation energy was determined to be 80 kJ/mol. The transport activity showed a marked dependence on the presence of Na+ ions and on the membrane potential, but was independent of an existing proton gradient. It is concluded, that uptake of branched-chain amino acid transport proceeds via a secondary active Na+-coupled symport mechanism.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Ebbighausen H,Weil B,Krämer Rdoi
10.1007/BF00413136subject
Has Abstractpub_date
1989-01-01 00:00:00pages
238-44issue
3eissn
0302-8933issn
1432-072Xjournal_volume
151pub_type
杂志文章abstract::An Arthrobacter sp. growing on 4-Chlorobenzoic acid as its sole source of carbon excretes 4-hydroxygenzoic acid and protocatechuic acid into the culture medium. Protocatechuic acid is further attacked by "meta"-cleavage. During growth of the Arthrobacter sp. on benzoic acid cis-cis muconic acid can be isolated from th...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00690235
更新日期:1976-11-02 00:00:00
abstract::Studies are presented of the biliproteins of Anabaena sp. This filamentous cyanobacterium contains three major biliproteins. Whereas two of these, C-phycocyanin and allophycocyanin, are common to all cyanobacteria, the third, phycoerythrocyanin (gammamax approximately 568 nm) has hitherto not been described and its di...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00416970
更新日期:1976-10-11 00:00:00
abstract::Cells of the yeast Saccharomycopsis capsularis fused in pairs after disolving of part of the cross wall between them near the lateral wall. After nuclear migrations through the opening, the cross wall was closed again and the cells at both sides became asci. The wall of the ascospores developed from a prospore wall. B...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447335
更新日期:1975-08-28 00:00:00
abstract::An isoeugenol-degrading enzyme was purified to homogeneity from Pseudomonas putida IE27, an isoeugenol-assimilating bacterium. The purified enzyme was a 55 kDa monomer and catalyzed the initial step of isoeugenol degradation, the oxidative cleavage of the side chain double-bond of isoeugenol, to form vanillin. Another...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0218-9
更新日期:2007-06-01 00:00:00
abstract::YfkE, a protein from Bacillus subtilis, exhibits homology to the Ca(2+):Cation Antiporter (CaCA) Family. In a fluorescence-based assay of everted membrane vesicles prepared from Na(+)(Ca(2+))/H(+) antiporter-defective mutant Escherichia coli KNabc, YfkE exhibited robust Ca(2+)/H(+) antiport activity, with a K (m) for ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0494-7
更新日期:2009-08-01 00:00:00
abstract::A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were reco...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00410734
更新日期:1984-04-01 00:00:00
abstract::A Gram-staining-negative, non-spore-forming, non-flagellated, rod-shaped, catalase- and oxidase-negative bacterium, designated as Gsoil 193(T), was isolated from the soil of ginseng field in Pocheon province, South Korea. 16S rRNA gene sequence analysis showed that strain Gsoil 193(T) belonged to family Xanthomonadace...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1214-8
更新日期:2016-08-01 00:00:00
abstract::In this study, Sorangium cellulosum So ce56 was phenotypically and genotypically analysed in order to evaluate whether this strain can be used in a comprehensive genome project as a representative of the secondary metabolite-producing myxobacteria. In contrast to many other strains of S. cellulosum, strain So ce56 was...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0479-2
更新日期:2002-12-01 00:00:00
abstract::We report on the genome-wide response, based on DNA microarrays, of the cyanobacterium Synechocystis sp. PCC 6803 wild type and DeltasigB to a 15 min heat shock. Approximately 9% of the genes in wild type and DeltasigB were significantly regulated (P < 0.001) following this treatment, with chaperones induced the most....
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-006-0138-0
更新日期:2006-10-01 00:00:00
abstract::We cloned the Kluyveromyces lactis KlNTH1 gene, which encodes neutral trehalase. It showed 65.2% and 68.5% identity at nucleotide and amino acid sequence level, respectively, with the Saccharomyces cerevisiae NTH1 gene. Multiple alignment of the predicted trehalase protein sequences from yeasts, bacteria, insects, and...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050436
更新日期:1997-04-01 00:00:00
abstract::A novel gram-negative, aerobic, non-spore-forming, rod-shaped and non-nitrogen fixing bacterium named strain 24NRT was isolated from wild Lilium pumilum bulbs in Fuping, Baoding City, Hebei province, PR China. The 16S rRNA gene sequences of strains 24NRT showed the highest similarity to Neorhizobium alkalisoli DSM 218...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01774-1
更新日期:2020-04-01 00:00:00
abstract::Twelve genes involved in thiamin biosynthesis in prokaryotes have been identified and overexpressed. Of these, six are required for the thiazole biosynthesis (thiFSGH, thil, and dxs), one is involved in the pyrimidine biosynthesis (thiC), one is required for the linking of the thiazole and the pyrimidine (thiE), and f...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030050713
更新日期:1999-04-01 00:00:00
abstract::The proteins in the culture supernatant (exoproteins) from Streptococcus pyogenes serotype M1 were separated by two-dimensional gel electrophoresis, and their N-terminal amino acid sequences were determined. The amino acid sequences were compared to sequences in the S. pyogenes genome database. The coding sequence sho...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0412-8
更新日期:2002-06-01 00:00:00
abstract::A Gram reaction-negative, strictly aerobic, non-motile, translucent and rod-shaped bacterium (designated W1-2-4(T)) isolated from tap water was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-4(T) was observed to grow optimally at 25-30 °C and at pH 6.5 on nutrient agar. Phylogene...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1237-1
更新日期:2016-10-01 00:00:00
abstract::Periodontal diseases are infectious polymicrobial inflammatory diseases that lead to destruction of the periodontal ligament, gingiva, and alveolar bone. Sequential colonization of a broad range of bacteria, including Fusobacterium nucleatum and Porphyromonas gingivalis, is an important phenomenon in this disease mode...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1223-7
更新日期:2016-08-01 00:00:00
abstract::The molar percentages of guanine plus cytosine in the DNA of 51 strains of Simonsiellaceae were determined by buoyant density ultracentrifugation of cell lysates in CsCl. The DNA base ratios ranged from 41-55 mole-% guanine plus cytosine. These values fall within the range known for the Order Cytophagales, the non-fru...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492026
更新日期:1977-06-20 00:00:00
abstract::1. An already published test method for detecting bactericidal substances in paper chromatograms was further improved. 2. In cultures of Cosmarium impressulum free from bacteria, two bactericidal substances were found in the ether extracts from the algae and two others in the extracts of the culture medium. The are ac...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00428945
更新日期:1976-06-01 00:00:00
abstract::The effects of the Ca(2+)/H(+) exchanger A23187 and the K(+)/H(+) exchanger nigericin, the electrogenic membrane-potential depleters valinomycin and CCCP, and the calcium channel blockers ruthenium red, nifedipine, and nitrendipine on the apical growth of Phycomyces blakesleeanus were analyzed. While all of the compou...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0609-5
更新日期:2003-12-01 00:00:00
abstract::Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00245225
更新日期:1990-01-01 00:00:00
abstract::After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492022
更新日期:1977-06-20 00:00:00
abstract::Bacillus cereus F4430/73 produced the highest levels of hemolysin BL (HBL) when grown under anaerobiosis in MOD medium. Anaerobic cells grown in a chemostat at low specific growth rate (0.1-0.2 h(-1)) expressed up to sevenfold more HBL than did cells held at a faster growth rate. At 0.2 h(-1), the presence of 90 mM gl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0688-y
更新日期:2004-09-01 00:00:00
abstract::Organic acid production including lactate and acetate is an economically attractive technology that has gained momentum worldwide over the past years. These series of action need to be performed by an esoteric and complex microbial community, in which different members have distinct roles in the establishment of a col...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01676-2
更新日期:2019-11-01 00:00:00
abstract::The chb operon of Escherichia coli is involved in the utilization of chitooligosaccharides. While acquisition of two classes of mutations leading to altered regulation of the chb operon is necessary to confer the ability to utilize the glucose disaccharide cellobiose to wild-type strains of E. coli, in the closely rel...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1299-0
更新日期:2017-03-01 00:00:00
abstract::Flow cytometry (FCM) in conjunction with immunocytochemical-labeling was used to analyze and screen a population of Escherichia coli clones containing a genomic library from the oil-degrading microorganism Acinetobacter calcoaceticus RAG-1 surface antigens. Reconstruction experiments using mixed populations indicated ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00422282
更新日期:1988-01-01 00:00:00
abstract::Recently, a unique archaeal/bacterial community that grows in a macroscopically visible string-of-pearls-like structure in cold (~10 degrees C), sulfurous marsh water was discovered. Here, a new technique is described that allows the fast and reliable growth of these string-of-pearls-like microbial communities in larg...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0580-1
更新日期:2003-09-01 00:00:00
abstract::Methanobacterium thermoautotrophicum growing on H2 plus CO2 as sole carbon and energy source was found to contain acetate thiokinase (Acetyl CoA synthetase; EC 6.2.1.1); Acetate + ATP + CoA leads to Acetyl CoA + AMP + PPi. The apparent Km value for acetate was 40 microM. Acetate kinase (EC 2.7.2.1) and phosphotransace...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406167
更新日期:1980-12-01 00:00:00
abstract::In the Tat protein export pathway of Gram-negative bacteria, TatA and TatB are homologous proteins that carry out distinct and essential functions in separate sub-complexes. In contrast, Gram-positive Tat systems usually lack TatB and the TatA protein is bifunctional. We have used a mutagenesis approach to delineate T...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-011-0699-4
更新日期:2011-08-01 00:00:00
abstract::RpoS, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. Many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. To determine the presence of this gene in enteric bacte...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100277
更新日期:2001-06-01 00:00:00
abstract::Beta-Lactamase (penicillinase) activity was found in a number of strains of blue-green algea. In some cases, this enzyme permitted algae to overcome the inhibitory effects of penicillin. Production and localization of beta-lactamase were studied in a unicellular species, Coccochloris elabens (strain 7003), and in a fi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429338
更新日期:1977-03-01 00:00:00
abstract::Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02133-1
更新日期:2020-12-15 00:00:00