Transport of branched-chain amino acids in Corynebacterium glutamicum.

Abstract:

:The transport of branched-chain amino acids was characterized in intact cells of Corynebacterium glutamicum ATCC 13032. Uptake and accumulation of these amino acids occur via a common specific carrier with slightly different affinities for each substrate (Km[Ile] = 5.4 microM, Km[Leu] = 9.0 microM, Km[Val] = 9.5 microM). The maximal uptake rates for all three substrates were very similar (0.94 - 1.30 nmol/mg dw.min). The optimum of amino acid uptake was at pH 8.5 and the activation energy was determined to be 80 kJ/mol. The transport activity showed a marked dependence on the presence of Na+ ions and on the membrane potential, but was independent of an existing proton gradient. It is concluded, that uptake of branched-chain amino acid transport proceeds via a secondary active Na+-coupled symport mechanism.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Ebbighausen H,Weil B,Krämer R

doi

10.1007/BF00413136

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

238-44

issue

3

eissn

0302-8933

issn

1432-072X

journal_volume

151

pub_type

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