Abstract：
:Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The isolated bacterium was identified as Exiguobaterium sp. strain AMBL-20T on the basis of morphological, biochemical, and phylogenetic analysis of 16S rRNA sequences with GenBank accession number MW229267. The bacterial strain was further screened for its lipolytic activity, biosynthesis, and characterization by different parameters with the aim of maximizing lipase activity. Results showed that 2% Olive oil, 0.2% peptone at 25 °C, pH 8, and 24 h of incubation time found optimal for maximum lipase production. The lipase enzyme was partially purified by ammonium sulphate precipitation and its activity was standardized at pH 8 under 30 °C temperature. The enzyme showed functional stability over a range of temperature and pH. Hence, extracellular alkaliphilic lipase from Exiguobacterium sp. is a potential candidate with extraordinary industrial applications, particularly in bio-detergent formulations.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Yasin MT,Ali Y,Ahmad K,Ghani A,Amanat K,Basheir MM,Faheem M,Hussain S,Ahmad B,Hussain A,Bokhari SAIdoi
10.1007/s00203-020-02133-1subject
Has Abstractpub_date
2020-12-15 00:00:00eissn
0302-8933issn
1432-072Xpii
10.1007/s00203-020-02133-1pub_type
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