Abstract：
:Methanobacterium thermoautotrophicum growing on H2 plus CO2 as sole carbon and energy source was found to contain acetate thiokinase (Acetyl CoA synthetase; EC 6.2.1.1); Acetate + ATP + CoA leads to Acetyl CoA + AMP + PPi. The apparent Km value for acetate was 40 microM. Acetate kinase (EC 2.7.2.1) and phosphotransacetylase (EC 2.3.1.8) could not be detected. The specific activity of acetate thiokinase was high in cells grown with limited H2 and CO2 supply (approximately 100 nmol/min . mg protein), it was low in exponentially grown cells (2 nmol/min . mg protein). This corresponded with the finding that cells growing linearly in the presence of acetate assimilated the monocarboxylic acid in high amounts (greater than 10% of the cell carbon was derived from acetate), whereas exponentially growing cells did not (less than 1% of cell carbon was derived from acetate). These latter observations indicated that acetate thiokinase and free acetate are not involved in autotrophic CO2 fixation in M. thermoautotrophicum. The presence and some kinetic properties of succinate thiokinase (EC 6.2.1.5), adenylate kinase (EC 2.7.4.3), and inorganic pyrophosphatase (EC 3.6.1.1) are also described.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Oberlies G,Fuchs G,Thauer RKdoi
10.1007/BF00406167subject
Has Abstractpub_date
1980-12-01 00:00:00pages
248-52issue
2eissn
0302-8933issn
1432-072Xjournal_volume
128pub_type
杂志文章abstract：:Saccharomyces cerevisiae mutants unable to grow at ethanol concentrations at which the wild type strain S288C does grow, have been isolated. Some of them show additional phenotypic alterations in colony size, temperature sensitivity and viability in ethanol, which cosegregate with the growth sensitivity in ethanol. 21...
journal_title：Archives of microbiology
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245367
更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-008-0445-8
更新日期：2009-03-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050308
更新日期：1996-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s00203-007-0341-7
更新日期：2008-06-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2020-10-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245305
更新日期：1993-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436326
更新日期：1975-03-12 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00409714
更新日期：1988-05-01 00:00:00
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journal_title：Archives of microbiology
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更新日期：1998-08-01 00:00:00
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更新日期：2013-01-01 00:00:00
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更新日期：1988-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-05-01 00:00:00
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更新日期：2020-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1982-12-03 00:00:00
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pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00411031
更新日期：1985-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436334
更新日期：1975-03-12 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1997-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-07-01 00:00:00
