Abstract:
:The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharide (KPS) biosynthesis, is constituted by the rkpU, rkpAGHIJ, and kpsF3 genes. Two mutants in this region affecting the rkpA (SVQ536) and rkpI (SVQ538) genes were constructed. Polyacrylamide gel electrophoresis and (1)H-NMR analyses did not detect KPS in these mutants. RT-PCR experiments indicated that, most probably, the rkpAGHI genes are cotranscribed. Glycine max cultivars (cvs.) Williams and Peking inoculated with mutants SVQ536 and SVQ538 showed reduced nodulation and symptoms of nitrogen starvation. Many pseudonodules were also formed on the American cv. Williams but not on the Asiatic cv. Peking, suggesting that in the determinate nodule-forming S. fredii-soybean symbiosis, bacterial KPS might be involved in determining cultivar-strain specificity. S. fredii HH103 mutants unable to produce KPS or exopolysaccharide (EPS) also showed reduced symbiotic capacity with Glycyrrhiza uralensis, an indeterminate nodule-forming legume. A HH103 exoA-rkpH double mutant unable to produce KPS and EPS was still able to form some nitrogen-fixing nodules on G. uralensis. Thus, here we describe for the first time a Sinorhizobium mutant strain, which produces neither KPS nor EPS is able to induce the formation of functional nodules in an indeterminate nodule-forming legume.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Margaret-Oliver I,Lei W,Parada M,Rodríguez-Carvajal MA,Crespo-Rivas JC,Hidalgo Á,Gil-Serrano A,Moreno J,Rodríguez-Navarro DN,Buendía-Clavería A,Ollero J,Ruiz-Sainz JE,Vinardell JMdoi
10.1007/s00203-011-0729-2subject
Has Abstractpub_date
2012-02-01 00:00:00pages
87-102issue
2eissn
0302-8933issn
1432-072Xjournal_volume
194pub_type
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