Abstract：
:Two intestinal bacterial strains MT4s-5 and MT42 involved in the degradation of (-)-epigallocatechin (EGC) were isolated from rat feces. Strain MT4s-5 was tentatively identified as Adlercreutzia equolifaciens. This strain converted EGC into not only 1-(3, 4, 5-trihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol (1), but also 1-(3, 5-dihydroxyphenyl)-3-(2, 4, 6-trihydroxyphenyl)propan-2-ol (2), and 4'-dehydroxylated EGC (7). Type strain (JCM 9979) of Eggerthella lenta was also found to convert EGC into 1. Strain MT42 was identified as Flavonifractor plautii and converted 1 into 4-hydroxy-5-(3, 4, 5-trihydroxyphenyl)valeric acid (3) and 5-(3, 4, 5-trihydroxyphenyl)-γ-valerolactone (4) simultaneously. Strain MT42 also converted 2 into 4-hydroxy-5-(3, 5-dihydroxyphenyl)valeric acid (5), and 5-(3, 5-dihydroxyphenyl)-γ-valerolactone (6). Furthermore, F. plautii strains ATCC 29863 and ATCC 49531 were found to catalyze the same reactions as strain MT42. Interestingly, formation of 2 from EGC by strain MT4s-5 occurred rapidly in the presence of hydrogen supplied by syntrophic bacteria. Strain JCM 9979 also formed 2 in the presence of the hydrogen or formate. Strain MT4s-5 converted 1, 3, and 4 to 2, 5, and 6, respectively, and the conversion was stimulated by hydrogen, whereas strain JCM 9979 could catalyze the conversion only in the presence of hydrogen or formate. On the basis of the above results together with previous reports, the principal metabolic pathway of EGC and EGCg by catechin-degrading bacteria in gut tract is proposed.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Takagaki A,Kato Y,Nanjo Fdoi
10.1007/s00203-014-1006-ysubject
Has Abstractpub_date
2014-10-01 00:00:00pages
681-95issue
10eissn
0302-8933issn
1432-072Xjournal_volume
196pub_type
杂志文章abstract：:The coloration of cells of the cyanobacterium Synechococcus sp. PCC 7002 changed from normal blue-green to yellow-green when cells were grown at 15 degrees C in a medium containing nitrate as the sole nitrogen source. This change of coloration was similar to a general response to nutrient deprivation (chlorosis). For ...
journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
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doi：10.1007/BF00402309
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406077
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0446-7
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pub_type： 杂志文章
doi：10.1007/s00203-015-1114-3
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journal_title：Archives of microbiology
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doi：10.1007/BF00245356
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doi：10.1007/s00203-020-02003-w
更新日期：2021-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01652-w
更新日期：2019-09-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050545
更新日期：1998-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454853
更新日期：1976-07-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00403209
更新日期：1980-03-01 00:00:00
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journal_title：Archives of microbiology
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pub_type： 杂志文章
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更新日期：2007-06-01 00:00:00
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更新日期：1994-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
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更新日期：1984-01-01 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447150
更新日期：1975-11-07 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00427847
更新日期：1977-10-24 00:00:00
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journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00415614
更新日期：1983-10-01 00:00:00
