Abstract:
:The prpC and prkC genes encode two proteins with opposing activities, eukaryotic-like protein phosphatase and protein kinase involved in the sporulation and biofilm formation processes. The prpC gene overlaps 3 bp of the prkC gene; prkC is followed by the essential gene, yloQ. The organisation of the prpC, prkC and yloQ genes is conserved among several Gram-positive bacteria. In this work, we have found two promoters which function within the region of these genes and we also described conditions in which these promoters become activated. One promoter appears to be located upstream of prpC resulting in transcription of both prpC and prkC, as well as the yloQ gene. A second promoter is located 7 bp upstream of the start codon of yloQ. The yloQ promoter was activated during ethanol stress, but was sigmaB-independent. We also showed that prpC, prkC and yloQ genes form an operon.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Iwanicki A,Hinc K,Seror S,Wegrzyn G,Obuchowski Mdoi
10.1007/s00203-005-0015-2keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
421-30issue
6eissn
0302-8933issn
1432-072Xjournal_volume
183pub_type
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