Abstract:
:The alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) of Pseudomonas fluorescens W was partially purified by (NH4)2SO4 fractionation, Sephadex G-200 and DEAE-cellulose column chromatography. The enzyme showed great specificity for maltose hydrolysis, with very little action against polymeric forms. Sucrose, isomaltose, alpha-methylglucoside, and maltobionic acid were not hydrolyzed. Turanose was a strong competitive inhibitor, and glucose a weaker one. Tris (2-amino-2-hydroxymethylpropan-1:3-diol) inhibited enzyme activity significantly only at alkaline pH. Mercuric, cupric, and silver cations strongly inhibited, and EDTA (ethylenediaminetetraacetate) weakly inhibited the enzyme. The isolated enzyme was rather unstable even at 4 degrees C, and was destroyed by freezing and lyophilization. Inositol and albumin had a slightly protective effect. Sulfhydryl-binding reagents strongly inhibited the enzyme.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Guffanti AA,Corpe WAdoi
10.1007/BF00425338subject
Has Abstractpub_date
1976-04-01 00:00:00pages
269-76issue
3eissn
0302-8933issn
1432-072Xjournal_volume
107pub_type
杂志文章abstract::The anaerobic fungus Piromyces sp. strain E2 produces extracellular cellulolytic enzymes present both in a high molecular mass (HMM) complex or as individual proteins. Although the HMM complex was present in the culture fluid during all growth stages, the highest amounts of complex were obtained when cultures were har...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::An unique isotope labelling experiment was conducted whereby mixtures of sulfate and sulfite of different isotopic compositions were metabolized by Clostridium pasteurianum. The results showed during reduction of 1 mM SO3= plus 1 mM SO4=, essentially all evolved H2S arose from the sulfite whereas in the case of cellul...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00446644
更新日期:1976-09-01 00:00:00
abstract::Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00414437
更新日期:1989-01-01 00:00:00
abstract::Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01999-5
更新日期:2021-01-01 00:00:00
abstract::Chloroquine inhibited the outgrowth of Bacillus subtilis spores at a 10-fold lower concentration than that required to prevent vegetative growth. Analysis of macromolecular synthesis in outgrowing spores and vegetative cells in the presence of chloroquine indicated that it acted preferentially on transcription. Differ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00409659
更新日期:1989-01-01 00:00:00
abstract::After the addition to soil of large numbers of a cowpea Rhizobium strain, the population declined steadily until the numbers reached about 10(7)/g, and the protozoa rose to about 10(4)/g. When indigenous protozoa were suppressed by the addition of actidione to the soil, the density of the test rhizobium did not fall i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00492022
更新日期:1977-06-20 00:00:00
abstract::The methanotrophic bacterium Methylomicrobium album BG8 uses methane as a sole source of carbon and energy. This bacterium forms an extensive intracytoplasmic membrane. The first enzymes of the methane oxidation pathway are the membrane-bound particulate methane monooxygenase and the periplasmic methanol dehydrogenase...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-002-0426-2
更新日期:2002-07-01 00:00:00
abstract::The gut microbiota inhabits the animal intestinal tract, and dysbiosis of the gut microbiota may result in disease. Senecio scandens has pharmaceutical antibacterial activities and is regarded as a broad-spectrum antibiotic in traditional Chinese medicine. Extracts of S. scandens are reported to show strong antimicrob...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-02144-y
更新日期:2021-01-04 00:00:00
abstract::Small heat-shock proteins are molecular chaperones that bind and prevent aggregation of nonnative proteins. They also associate with membranes. In this study, we show that the small heat-shock protein HspA plays a protective role under oxidative stress in the cyanobacterium Synechococcus elongatus strain ECT16-1, whic...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0457-z
更新日期:2009-04-01 00:00:00
abstract::The glutamate catabolism of three thermophilic syntrophic anaerobes was compared based on the combined use of [(13)C] glutamate NMR measurements and enzyme activity determinations. In some cases the uptake of intermediates from different pathways was studied. The three organisms, Caloramator coolhaasii, Thermanaerovib...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030100286
更新日期:2001-07-01 00:00:00
abstract::1. Culture filtrates of heterotrophic bacteria were tested for their stimulatory effect on nitrification of three strains of Nitrobacter. 2. Yeast extract-peptone solution, in which Pseudomonas fluorescens had grown, after removal of the cells was added to autotrophically growing cultures of Nitrobacter agilis; it cau...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454856
更新日期:1976-07-01 00:00:00
abstract::NAD-linked lactate dehydrogenases specific for the D- and L-lactate have been demonstrated in a number of strains of unicellular cyanobacteria. The D-lactate dehydrogenase of one strain (Synechococcus 6716) was partially purified and its properties were studied. The enzyme has a molecular weight of ca. 115000-120000...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447300
更新日期:1975-06-20 00:00:00
abstract::Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Cal...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00413014
更新日期:1984-06-01 00:00:00
abstract::Nitrosomonas europaea, a Gram-negative obligate chemolithoautotroph, participates in global nitrogen cycling by carrying out nitrification and derives energy for growth through oxidation of ammonia. In this work, the physiological, proteomic, and transcriptional responses of N. europaea to zinc stress were studied. Th...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0341-7
更新日期:2008-06-01 00:00:00
abstract::The attachment of four species of bacteria to solid surfaces was investigated to determine whether the attachment of one species of bacterium could be influenced by the presence of other attaching or attached species. Three types of experiment were done: attachment of bacteria from suspensions containing two species (...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429648
更新日期:1987-06-01 00:00:00
abstract::The main aim of our study was to determine the physiological function of NagA enzyme in the Listeria monocytogenes cell. The primary structure of the murein of L. monocytogenes is very similar to that of Escherichia coli, the main differences being amidation of diaminopimelic acid and partial de-N-acetylation of gluco...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-011-0752-3
更新日期:2012-04-01 00:00:00
abstract::The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00381781
更新日期:1995-02-01 00:00:00
abstract::Chemical mutagenesis of the nucleotide-producing strain Corynebacterium ammoniagenes ATCC 6872 with N-methyl-N-nitro-N-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. A rapid assay for the allosterically regulated Mn-ribonucleotide reductase (RRase) was developed with ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050450
更新日期:1997-05-01 00:00:00
abstract::In studies on the membranlytic action of various saponins on mycelium of Botrytis cinerea and Rhizoctonia solani digitonin, alpha-hederin and tomatin caused considerable leakage of free amino acids, while aescin and theasaponin were less effective. Cyclamin significantly damaged cell membranes of R. solani, but did no...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00447315
更新日期:1975-06-22 00:00:00
abstract::The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1222-8
更新日期:2016-09-01 00:00:00
abstract::Of 147 microorganisms isolated from a loamy sand, 71 showed good growth with lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane) and produced chloride in an aqueous medium. Thirteen soil microorganisms were selected to study the utilization of lindane. Lindane was metabolized by the microbes to gamma-2,3,4,5,6-pentachlo...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00454850
更新日期:1976-07-01 00:00:00
abstract::Multi- and pan-antibiotic-resistant bacteria area major health challenge in hospital settings. Furthermore,when susceptible bacteria establish surface-attached biofilm populations, they become recalcitrant to antimicrobial therapy. Therefore, there is a need for novel antimicrobials that are effective against multi-dr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-012-0793-2
更新日期:2012-07-01 00:00:00
abstract::The symbiotic bacteria Bradyrhizobium japonicum and Sinorhizobium meliloti, and the purple photosynthetic bacteria Rhodobacter capsulatus, Rhodovulum sulfidophilum, Roseobacter denitrificans and Rhodobacter sphaeroides possess homologous two-component regulatory systems, namely RegSR, ActSR, RegBA and PrrBA. The respe...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030000207
更新日期:2000-11-01 00:00:00
abstract::Only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. Little is known about the dietary and physiological factors determining this ecological feature. In this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was i...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-004-0747-4
更新日期:2005-01-01 00:00:00
abstract::The upstream region of the isocitrate lyase gene (UPR-ICL, 1530bp) of an n-alkane-utilizable yeast, Candida tropicalis, induced gene expression in another yeast, Saccharomyces cerevisiae, when the yeasts were grown on acetate. Surprisingly, UPR-ICL displayed the same regulatory function in the bacterium Escherichia co...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00404204
更新日期:1995-05-01 00:00:00
abstract::In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to be competitive inhi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00411361
更新日期:1979-08-06 00:00:00
abstract::The histidine protein kinase SppK is a peptide pheromone-activated kinase that regulates the production of the bacteriocin sakacin P in Lactobacillus sakei. SppK belongs to subfamily 10 of histidine protein kinases (HPKs), which regulate important processes in Gram-positive bacteria, including virulence, competence an...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-005-0049-5
更新日期:2006-01-01 00:00:00
abstract::In view of the functional similarities between subtilisin Carlsberg and the alkaline protease from Conidiobolus coronatus, the biochemical and structural properties of the two enzymes were compared. In spite of their similar biochemical properties, e.g., pH optima, heat stability, molecular mass, pI, esterase activity...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF01682989
更新日期:1996-12-01 00:00:00
abstract::Outer membrane vesicles (OMVs) are released from the outer membrane of Gram-negative bacteria. Moreover, Gram-positive bacteria also produce membrane-derived vesicles. As OMVs transport several bacterial components, especially from the cell envelope, their interaction with the host cell, with other bacteria or as immu...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s00203-014-1042-7
更新日期:2015-01-01 00:00:00
abstract::The bacteriocinogenic strain of Enterococcus faecalis EJ97 has been isolated from municipal waste water. It produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,340 Da) that is very stable under mild heat conditions and is sensitive to proteolytic enzymes. The amino acid sequence of the first 18 N...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050678
更新日期:1998-12-01 00:00:00