Abstract：
:Multi- and pan-antibiotic-resistant bacteria area major health challenge in hospital settings. Furthermore,when susceptible bacteria establish surface-attached biofilm populations, they become recalcitrant to antimicrobial therapy. Therefore, there is a need for novel antimicrobials that are effective against multi-drug-resistant and surface-attached bacteria. A screen to identify prokaryote-derived antimicrobials from a panel of over 100 bacterial strains was performed. One compound isolated from Citrobacter freundii exhibited antimicrobial activity against a wide range of Gram-negative bacteria and was effective against biofilms. Random transposon mutagenesis was performed to find mutants unable to produce the antimicrobial compound.Transposons mapped to a bacteriocin gene located on a small plasmid capable of replication in Escherichia coli. The plasmid was sequenced and found to be highly similar to a previously described colicinogenic plasmid.Expression of the predicted bacteriocin immunity gene conferred bacteriocin immunity to E. coli. The predicted bacteriocin gene, colA-43864, expressed in E. coli was sufficient to generate anti-microbial activity, and purified recombinant ColA-43864 was highly effective in killing E. coli, Citrobacter species, and Klebsiella pneumoniae cells in a planktonic and biofilm state. This study suggests that bacteriocins can be an effective way to control surface-attached pathogenic bacteria.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Shanks RM,Dashiff A,Alster JS,Kadouri DEdoi
10.1007/s00203-012-0793-2subject
Has Abstractpub_date
2012-07-01 00:00:00pages
575-87issue
7eissn
0302-8933issn
1432-072Xjournal_volume
194pub_type
杂志文章abstract：:Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0238-5
更新日期：2007-08-01 00:00:00
abstract：:The coloration of cells of the cyanobacterium Synechococcus sp. PCC 7002 changed from normal blue-green to yellow-green when cells were grown at 15 degrees C in a medium containing nitrate as the sole nitrogen source. This change of coloration was similar to a general response to nutrient deprivation (chlorosis). For ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050535
更新日期：1998-01-01 00:00:00
abstract：:The ADPglucose pyrophosphorylases from Rhodopseudomonas sphaeroides and Rhodopseudomonas gelatinosa are activated by fructose-6-phosphate, pyruvate and fructose-1,6 biophosphate-P2. The effects of the activators are to increase significantly the Vmax of ADPglucose synthesis and to lower the S0.5 values (concentration ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00421887
更新日期：1980-05-01 00:00:00
abstract：:During the development of fruit bodies of the basidiomycete Schizophyllum commune, the alkali-insoluble (R glucan) and alkali-soluble (S glucan) cell wall fractions are synthesized during the entire course of morphogenesis. The water soluble glucan (WSG) is not synthesized after an early stage. There is also a relativ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429623
更新日期：1977-07-26 00:00:00
abstract：:To identify virulence-associated genes of a fish pathogen Yersinia ruckeri, we screened a total of 1056 mini-Tn5-Km2 signature-tagged mutants in rainbow trout by immersion challenge. Of 1056, 25 mutants were found survival-defective as they could not be re-isolated from fish kidney 7 days after infection. Mutated gene...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0582-8
更新日期：2010-07-01 00:00:00
abstract：:Saccharomyces cerevisiaepresents two alternative vegetative forms of growth, switching between yeast forms to pseudohyphal forms depending on the specific environmental conditions. To identify genes involved in cell wall morphogenesis, a haploid S. cerevisiae monomorphic mutant, W27, which exhibits pseudohyphal growth...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0400-z
更新日期：2002-04-01 00:00:00
abstract：:Klebsiella pneumoniae NCTC 418 was cultured aerobically in chemostat cultures (D = 0.3 h-1; 35 degrees C) under respectively carbon-, phosphate-, potassium-, sulphate-, and ammonia-limited conditions with glucose as the sole carbon and energy source. The effect of the external pH value on glucose metabolism and on the...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413140
更新日期：1989-01-01 00:00:00
abstract：:VcrD1 protein is a component of type III secretion system (T3SS) 1 in Vibrio parahaemolyticus. A comparative analysis of secretomes of wild-type and ΔvcrD1 strains revealed that the mutant was defective in secretion of diverse proteins including several flagellar components. Western blot analyses using specific antibo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-014-1069-9
更新日期：2015-04-01 00:00:00
abstract：:Microorganisms have evolved themselves to thrive under various extreme environmental conditions such as extremely high or low temperature, alkalinity, and salinity. These microorganisms adapted several metabolic processes to survive and reproduce efficiently under such extreme environments. As the major proportion of ...
journal_title：Archives of microbiology
pub_type： 杂志文章,评审
doi：10.1007/s00203-018-1602-3
更新日期：2019-01-01 00:00:00
abstract：:Anguibactin, an important virulent factor in Vibrio anguillarum serotype O1, is synthesized by a nonribosomal peptide synthetases (NRPS) system encoded on a 65-kb virulence plasmid pJM1. angE, as one of the NRPS genes, is responsible for selecting and activating 2,3-dihydroxybenzoic acid (2,3-DHBA), an important precu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0652-x
更新日期：2004-04-01 00:00:00
abstract：:Photosynthetic, nitrogen-fixing Anabaena strains play an important role in the carbon and nitrogen cycles in tropical paddy fields although they are salt sensitive. Improvement in salt tolerance of Anabaena cells by expressing glycine betaine-synthesizing genes is an interesting subject. Due to the absence of choline ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0824-z
更新日期：2012-11-01 00:00:00
abstract：:The biological effects of UV radiation of different wavelengths (UVA, UVB and UVC) were assessed in nine bacterial isolates displaying different UV sensitivities. Biological effects (survival and activity) and molecular markers of oxidative stress [DNA strand breakage (DSB), generation of reactive oxygen species (ROS)...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0847-5
更新日期：2013-01-01 00:00:00
abstract：:The production of secondary metabolites with antibiotic properties is a common characteristic to Bacillus spp. These metabolites not only have diverse chemical structures but also have a wide range of bioactivities with medicinal and agricultural interests such as antibiotic. Bacillus sp. fmbJ has been found to produc...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0939-x
更新日期：2014-02-01 00:00:00
abstract：:Curcumin is a yellow-orange compound derived from the root of Curcuma longa (Zingiberaceae family), that has been used as a medicine, spice and coloring agent. Curcumin has proved nontoxic in a number of cell culture and whole animal studies. Curcumin has, however, been reported to have bactericidal effects at very hi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00414437
更新日期：1989-01-01 00:00:00
abstract：:Ever since the development of the first antibiotic compound with anticancer potential, researchers focused on isolation and characterization of prospective microbial natural products with potential anti-infective and anticancer activities. The present work describes the production of bioactive metabolites by heterotro...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02106-4
更新日期：2020-11-02 00:00:00
abstract：:Organic acid production including lactate and acetate is an economically attractive technology that has gained momentum worldwide over the past years. These series of action need to be performed by an esoteric and complex microbial community, in which different members have distinct roles in the establishment of a col...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01676-2
更新日期：2019-11-01 00:00:00
abstract：:Of the seven amino acids required by Clostridium botulinum type E, tryptophan is the most essential and may provide the cell with nitrogen. The addition of excess tryptophan (10-20 mM) or other nitrogenous nutrients to minimal growth medium markedly decreased toxin formation but did not affect growth in C. botulinum t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245225
更新日期：1990-01-01 00:00:00
abstract：:A new method is described for the quantitative microbiological analysis of the thiamine analog pyrithiamine present in biological material. The method uses a mutant of Lactobacillus fermenti (ATCC 9338) stimulated by pyrithiamine. By this specific method it is found that Serratia marcescens is able to consume and to p...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00436334
更新日期：1975-03-12 00:00:00
abstract：:To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0406-2
更新日期：2008-11-01 00:00:00
abstract：:A Gram-negative, yellow-colored, rod-shaped, strictly aerobic, motile bacterium was isolated from forest soil in Gyeongsang Province, South Korea. The strain was designated as THG-DA2.1(T). Cells of strain THG-DA2.1(T) grew optimally at pH 7.0, at temperature 28 °C and in the presence of 0.5 % NaCl. Based on 16S rRNA ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-015-1114-3
更新日期：2015-08-01 00:00:00
abstract：:Mangrove sediment-associated bacteria are of significantly important in the field of medicine and pharmaceuticals as new promising sources of biologically active pharmacophores due to the extreme conditions, such as high salt concentration and soil anoxia. The sediment bacteria associated with Acanthus ilicifolius and...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-01999-5
更新日期：2021-01-01 00:00:00
abstract：:We have investigated the endogenous phosphorylation patterns of phosphorylated proteins of Xanthomonas campestris pv. oryzae induced by its bacteriophages. For bacteriophage Xp12-infected cells, at least three phosphoproteins with apparent molecular weights of 28, 28.5 and 45 kDa were detected by in vitro labeling wit...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00303581
更新日期：1994-01-01 00:00:00
abstract：:When Clostridium formicoaceticum was grown on fumarate or L-malate crude cell extracts contained a high fumarate reductase activity. Using reduced methyl viologen as electron donor the specific activity amounted to 2-3.5 U per mg of protein. Reduced benzyl viologen, FMNH2 and NADH could also serve as electron donors b...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407920
更新日期：1978-10-04 00:00:00
abstract：:The clones generated in a sequencing project represent a resource for subsequent analysis of the organism whose genome has been sequenced. We describe an interrelated group of cloning vectors that either integrate into the genome or replicate, and that enhance the utility, for developmental and other studies, of the c...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-007-0276-z
更新日期：2007-12-01 00:00:00
abstract：:Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mas...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00262202
更新日期：1995-01-01 00:00:00
abstract：:A bacterium capable of using the carbamate insecticide carbofuran as a sole source of carbon and energy, was isolated from soil. The ability to catabolise carbofuran phenol, produced by cleavage of the carbamate ester linkage of the insecticide, was lost at very high frequency when the bacterium was grown in the absen...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00245249
更新日期：1992-01-01 00:00:00
abstract：:The sulfate-reducing highly enriched culture N47 is capable to anaerobically degrade naphthalene, 2-methylnaphthalene, and 2-naphthoic acid. A proteogenomic investigation was performed to elucidate the initial activation reaction of anaerobic naphthalene degradation. This lead to the identification of an alpha-subunit...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0667-4
更新日期：2011-04-01 00:00:00
abstract：:Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant ΔpknE. The survival of ΔpknE was assessed in the presence of stress (pH, surfa...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-012-0848-4
更新日期：2013-01-01 00:00:00
abstract：:Two ornithine carbamoyltransferases (OCT 1 and OCT 2) were isolated from Pseudomonas syringae pv. phaseolicola and purified by precipitation with ammonium sulfate, heat denaturation, chromatography on DEAE-Sephadex A-50 and Sephadex G-200. Molecular weights of both enzymes: 110,000; optimal activity: pH 8.5 to 9.5 (OC...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00415280
更新日期：1987-03-01 00:00:00
abstract：:The subtype of phosphofructokinase activity, either ATP-, ADP- or pyrophosphate-dependent, present in members of three genera from the Spirochaetales was investigated. The individual species/strains examined included Spirochaeta alkalica, S. asiatica, S. halophila, S. isovalerica, S. litoralis, S. zuelzerae, S. thermo...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050777
更新日期：1999-12-01 00:00:00
