Abstract：
:Pseudomonas maltophilia CSV89, a bacterium isolated from soil in our laboratory, grows on 1-naphthoic acid as the sole source of carbon and energy. To elucidate the pathway for degradation of 1-naphthoic acid, the metabolites were isolated from spent medium, purified by TLC, and characterized by gas chromatography-mass spectrometry. The involvement of various metabolites as intermediates in the pathway was established by demonstrating relevant enzyme activities in cell-free extracts, oxygen uptake and transformation of metabolites by the whole cells. The results obtained from such studies suggest that the degradation of 1-naphthoic acid is initiated by double hydroxylation of the aromatic ring adjacent to the one bearing the carboxyl group, resulting in the formation of 1,2-dihydroxy-8-carboxynaphthalene. The resultant diol was oxidized via 3-formyl salicylate, 2-hydroxyisophthalate, salicylate and catechol to TCA cycle intermediates.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Phale PS,Mahajan MC,Vaidyanathan CSdoi
10.1007/BF00262202subject
Has Abstractpub_date
1995-01-01 00:00:00pages
42-7issue
1eissn
0302-8933issn
1432-072Xjournal_volume
163pub_type
杂志文章abstract：:The time dependent assembly of the photosynthetic apparatus was studied in Rhodospirillum rubrum after transfer of cells growing aerobically in the dark to low aeration. While bacteriochlorophyll (Bchl) cellular levels increase continuously levels of soluble cytochrome C2 do not change significantly. Absorption spectr...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00454853
更新日期：1976-07-01 00:00:00
abstract：:Synechococcus elongatus strain PCC7942 cells were grown in high or low environmental concentrations of inorganic C (high-C(i), low-C(i)) and subjected to a light shift from 50 micromol m(-2) s(-1) to 500 micromol m(-2) s(-1). We quantified photosynthetic reductant (O(2) evolution) and molar cellular contents of phycob...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-005-0761-1
更新日期：2005-03-01 00:00:00
abstract：:Chemical mutagenesis of the nucleotide-producing strain Corynebacterium ammoniagenes ATCC 6872 with N-methyl-N-nitro-N-nitrosoguanidine followed by an enrichment protocol yielded 46 temperature-sensitive (ts) clones. A rapid assay for the allosterically regulated Mn-ribonucleotide reductase (RRase) was developed with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050450
更新日期：1997-05-01 00:00:00
abstract：:L-(+)-lactate dehydrogenase (LDH) from Staphylococcus epidermidis ATCC 14990 was purified by affinity chromatography. The purified enzyme was specifically activated by fructose-1,6-diphosphate (FDP). The concentration of FDP required for 50% maximal activity was about 0.15 mM. The enzyme activity was inhibited by aden...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447150
更新日期：1975-11-07 00:00:00
abstract：:Plasmid pJP4 encoding the ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (Tfd+) was transferred by conjugation from Escherichia coli JMP397 to various lithoautotrophic strains of Alcaligenes eutrophus and to the autotrophic bacterium Pseudomonas oxalaticus. The herbicide-degrading function of the plas...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00407938
更新日期：1983-02-01 00:00:00
abstract：:The entomopathogenic mushroom Cordyceps militaris is a storehouse of various medicinal compounds and pharmacological effects. However, the high frequency of strain degeneration during subculture and preservation severely limits the large-scale production of C. militaris. DNA methylation is an important epigenomic modi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01621-3
更新日期：2019-04-01 00:00:00
abstract：:Coenzyme F420 is a 8-hydroxy-5-deazaflavin present in methanogenic bacteria. We have investigated whether the pyrimidine ring of the deazaflavin originates from guanine as in flavin biosynthesis, in which the pyrimidine ring of guanine is conserved. For this purpose the incorporation of [2-14C]guanine and of [8-14C]gu...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00410735
更新日期：1984-04-01 00:00:00
abstract：:Upland rice is an ecotype crop resulting from the long-term domestication and evolution of rice in dry land without a water layer. Generally, the stems and leaves are thick and luxuriant, while the leaves also typically broad and light. The root system is developed with abundant root hair, and the osmotic pressure of ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02058-9
更新日期：2020-09-29 00:00:00
abstract：:A gram-negative bacterium was isolated from activated sewage sludge with thiophene-2-carboxylate as the sole source of carbon and with nitrate as an electron acceptor. The isolate, strain NKK, was a motile, oxidase- and catalase-positive, rod-like bacterium with a G+C content of 61.7 mol%. Besides nitrate, oxygen coul...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050575
更新日期：1998-04-01 00:00:00
abstract：:Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titrat...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02154-w
更新日期：2021-01-05 00:00:00
abstract：:The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-beta-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-beta-glucanase, alpha-amylase and beta-glucosidase activities....
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00425066
更新日期：1979-06-01 00:00:00
abstract：:In order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (FadBA) in the provision of (R)-3-hydroxyacyl-CoA thioesters for medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis, the fadBA(Po) operon of Pseudomonas oleovorans was cloned and characterized. T...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-002-0444-0
更新日期：2002-08-01 00:00:00
abstract：:To elucidate the physiological adaptation of Escherichia coli due to cra gene knockout, a total of 3,911 gene expressions were investigated by DNA microarray for continuous culture. About 50 genes were differentially regulated for the cra mutant. TCA cycle and glyoxylate shunt were down-regulated, while pentose phosph...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-008-0406-2
更新日期：2008-11-01 00:00:00
abstract：:Comparative 16S rRNA gene sequence and genomic DNA reassociation analyses were used to assess the phylogenetic relationships of Methanobrevibacter fecal isolates. The 16S rRNA gene sequences of Methanobrevibacter smithii strain PS and the human fecal isolates B181 and ALI were essentially identical, and their genomic ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s002030050589
更新日期：1998-05-01 00:00:00
abstract：:A lack on the association between acid tolerance response (ATR) and osmotolerance response (OTR) among Listeria monocytogenes dairy isolates was found. In order to evaluate how wild L. monocytogenes isolates mount tolerance responses under a sub-lethal pH and a low sodium chloride concentration (pH 5.5 and 3.5 % [w/v]...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-013-0878-6
更新日期：2013-05-01 00:00:00
abstract：:Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-017-1415-9
更新日期：2018-01-01 00:00:00
abstract：:The biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in Saccharomyces cerevisiae the structural gene coding for beta-glucosidase in Kluyveromyces fragilis. The enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. These features are similar to t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00402336
更新日期：1986-11-01 00:00:00
abstract：:Methanobacterium thermoautotrophicum growing on H2 plus CO2 as sole carbon and energy source was found to contain acetate thiokinase (Acetyl CoA synthetase; EC 6.2.1.1); Acetate + ATP + CoA leads to Acetyl CoA + AMP + PPi. The apparent Km value for acetate was 40 microM. Acetate kinase (EC 2.7.2.1) and phosphotransace...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406167
更新日期：1980-12-01 00:00:00
abstract：:Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-019-01618-y
更新日期：2019-03-01 00:00:00
abstract：:CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28 degrees C; but at 4 degrees C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was tra...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-010-0591-7
更新日期：2010-08-01 00:00:00
abstract：:Both the changes in the activities of nitrogenase, glutamine synthetase and glutamate dehydrogenase and in the extracellular and intracellular NH4+ concentrations were investigated during the transition from an NH4+ free medium to one containing NH4+ ions for a continuous culture of Azotobacter vinelandii. If added in...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00447319
更新日期：1975-06-22 00:00:00
abstract：:Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-020-02133-1
更新日期：2020-12-15 00:00:00
abstract：:It has been shown previously that externally added glycine betaine is accumulated in Escherichia coli in response to the external osmotic strength. Here we have shown, by using nuclear magnetic resonance spectroscopy and radiochemical methods, that E. coli growing in a glucose-mineral medium of elevated osmotic streng...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00492896
更新日期：1987-02-01 00:00:00
abstract：:Polysomes were isolated both from growing gametophytes of Allomyces arbuscula and from gametangia prepared from mycelia at different periods during gametogenesis. Analysis of polysomes by sucrose gradients showed that ribosomes present in the gametangia monosome pool were shifted into polysomes. This shift was found t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00413089
更新日期：1977-04-01 00:00:00
abstract：:The rubredoxin content of Acinetobacter calcoaceticus in dependence on the carbon source (acetate, n-alkanes, succinate, L-malate) and on the growth phase was studied by means of a radioimmunoassay. The method used was specific for rubredoxin and Acinetobacter calcoaceticus. The formation of rubredoxin increased with ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00406170
更新日期：1980-12-01 00:00:00
abstract：:DNA sequence analysis of the structural urease genes from Staphylococcus xylosus revealed that three enzyme subunits are encoded in the order of 11,000, 15,400 and 61,000 (mol. mass), which correspond to the single polypeptide chain of jack bean urease (90,800). Comparing the deduced amino acid sequence of S. xylosus ...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00288947
更新日期：1994-01-01 00:00:00
abstract：:Aerobic anoxygenic phototrophs (AAPs) are prokaryotic microorganisms capable of harvesting light using bacteriochlorophyll-based reaction centres. Marine AAP communities are generally dominated by species belonging to the Roseobacter clade. For this reason, we used marine Roseobacter-related strain COL2P as a model or...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-009-0529-0
更新日期：2010-01-01 00:00:00
abstract：:The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was t...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00381781
更新日期：1995-02-01 00:00:00
abstract：:Beta-Lactamase (penicillinase) activity was found in a number of strains of blue-green algea. In some cases, this enzyme permitted algae to overcome the inhibitory effects of penicillin. Production and localization of beta-lactamase were studied in a unicellular species, Coccochloris elabens (strain 7003), and in a fi...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/BF00429338
更新日期：1977-03-01 00:00:00
abstract：:A moderately psychrophilic purple non-sulfur bacterium, Rhodoferax antarcticus strain Fryx1, is described. Strain Fryx1 was isolated from the water column under the ice of the permanently frozen Lake Fryxell, Antarctica. Cells of Fryx1 are long thin rods and contain gas vesicles, the first report of such structures in...
journal_title：Archives of microbiology
pub_type： 杂志文章
doi：10.1007/s00203-004-0719-8
更新日期：2004-10-01 00:00:00
