Biosynthesis regulation of the beta-glucosidase produced by a yeast strain transformed by genetic engineering.

Abstract:

:The biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in Saccharomyces cerevisiae the structural gene coding for beta-glucosidase in Kluyveromyces fragilis. The enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. These features are similar to those observed in K. fragilis. beta-Glucosidase activity in the transformed yeast was much higher than in K. fragilis. We attempted to ferment cellobiose with the transformed yeast: practically no cellobiose was consumed, growth and ethanol production were negligible. Warburg experiments showed that cellobiose fermentation did not occur when the respiratory chain was not functioning.

journal_name

Arch Microbiol

journal_title

Archives of microbiology

authors

Leclerc M,Chemardin P,Arnaud A,Ratomahenina R,Galzy P,Gerbaud C,Raynal A

doi

10.1007/BF00402336

subject

Has Abstract

pub_date

1986-11-01 00:00:00

pages

115-7

issue

2

eissn

0302-8933

issn

1432-072X

journal_volume

146

pub_type

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