Abstract:
:The biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in Saccharomyces cerevisiae the structural gene coding for beta-glucosidase in Kluyveromyces fragilis. The enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. These features are similar to those observed in K. fragilis. beta-Glucosidase activity in the transformed yeast was much higher than in K. fragilis. We attempted to ferment cellobiose with the transformed yeast: practically no cellobiose was consumed, growth and ethanol production were negligible. Warburg experiments showed that cellobiose fermentation did not occur when the respiratory chain was not functioning.
journal_name
Arch Microbioljournal_title
Archives of microbiologyauthors
Leclerc M,Chemardin P,Arnaud A,Ratomahenina R,Galzy P,Gerbaud C,Raynal Adoi
10.1007/BF00402336subject
Has Abstractpub_date
1986-11-01 00:00:00pages
115-7issue
2eissn
0302-8933issn
1432-072Xjournal_volume
146pub_type
杂志文章abstract::Elevation gradients, often regarded as "natural experiments or laboratories", can be used to study changes in the distribution of microbial diversity related to changes in environmental conditions that typically occur over small geographical scales. We exploited this feature by characterizing fungal composition and di...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01948-2
更新日期:2020-10-01 00:00:00
abstract::The Roseobacter clade, belonging to the family Rhodobacteraceae of the class Alphaproteobacteria, is one of the major bacterial groups in marine environments. A remarkable wealth of diverse large plasmids has been detected in members of this lineage. Here, we analysed the genome structure and extrachromosomal DNA cont...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0535-2
更新日期:2010-02-01 00:00:00
abstract::Catalytic properties and membrane associations of the phosphatidylglycerophosphate (PGP) and phosphatidylserine (PS) synthases of Rhodobacter sphaeroides were examined to further characterize sites of phospholipid biosynthesis. In preparations of cytoplasmic membrane (CM) enriched in these activities, apparent Km valu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00456090
更新日期:1989-01-01 00:00:00
abstract::The main property of an Amt- (ammonium transport negative) mutant of Klebsiella pneumoniae is its inability to accumulate NH4+ intracellularly. When growing on nitrogen sources other than NH4+, the mutant constantly looses NH3 by diffusion. This loss results in poor growth. The NH3 excretion suggests the existence of ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402008
更新日期:1984-10-01 00:00:00
abstract::The regulation of the syntheses of two arabinan-degrading extracellular enzymes and several intracellular L-arabinose catabolic enzymes was examined in wild-type and carbon catabolite derepressed mutants of Aspergillus nidulans. alpha-L-Arabinofuranosidase B, endoarabinase, L-arabinose reductase, L-arabitol dehydrogen...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00282109
更新日期:1994-01-01 00:00:00
abstract::The maleylacetate reductase of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 has been purified 50-fold. The enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleyl...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00276301
更新日期:1992-01-01 00:00:00
abstract::Polyhydroxyalkanoates (PHAs) are biological polyesters, of which, Short-Chain-Length-Medium-Chain-Length (SCL-MCL) PHA copolymers are important because of their wide range of applications. The present study focused on molecular characterization of Pseudomonas sp. LDC-5 that is identified as SCL-MCL producer. Phase con...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-007-0265-2
更新日期:2007-11-01 00:00:00
abstract::The control of pyrimidine nucleotide formation in the bacterium Pseudomonas aurantiaca ATCC 33663 by pyrimidines was studied. The activities of the pyrimidine biosynthetic pathway enzymes were investigated in P. aurantiaca ATCC 33663 cells and from cells of an auxotroph lacking orotate phosphoribosyltransferase activi...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-020-01842-x
更新日期:2020-08-01 00:00:00
abstract::Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00444661
更新日期:1989-01-01 00:00:00
abstract::The chb operon of Escherichia coli is involved in the utilization of chitooligosaccharides. While acquisition of two classes of mutations leading to altered regulation of the chb operon is necessary to confer the ability to utilize the glucose disaccharide cellobiose to wild-type strains of E. coli, in the closely rel...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1299-0
更新日期:2017-03-01 00:00:00
abstract::Milk containing antibiotics is used as cost-effective feed for calves, which may lead to antibiotic residues-associated food safety problems. This study aims to investigate the influence of antibiotics on rumen microbes. Through metagenomic sequencing, the rumen microbial communities of calves fed with pasteurized mil...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1311-8
更新日期:2017-04-01 00:00:00
abstract::CO2 is required continuously during germination of Streptomyces viridochromogenes spores. Spores incubated in a defined germination medium in the absence of CO2 remain phase bright and do not release spore carbon. In the presence of CO2, the spores initiate germination accompanied by loss of refractility and spore car...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00429118
更新日期:1978-09-01 00:00:00
abstract::A Gram-negative, yellow-colored, rod-shaped, strictly aerobic, motile bacterium was isolated from forest soil in Gyeongsang Province, South Korea. The strain was designated as THG-DA2.1(T). Cells of strain THG-DA2.1(T) grew optimally at pH 7.0, at temperature 28 °C and in the presence of 0.5 % NaCl. Based on 16S rRNA ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-015-1114-3
更新日期:2015-08-01 00:00:00
abstract::The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was de...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-009-0533-4
更新日期:2010-02-01 00:00:00
abstract::The carboxysomal polypeptides of Thiobacillus neapolitanus with apparent molecular masses of 85 and 130 kDa were isolated and subjected to N-terminal sequencing. The first 17 amino acids of the two peptides were identical. The sequence perfectly matched the deduced amino acid sequence of an open reading frame in the c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050765
更新日期:1999-10-01 00:00:00
abstract::Oxalobacter formigenes (O. formigenes) is a nonpathogenic, Gram-negative, obligate anaerobic bacterium that commonly inhabits the human gut and degrades oxalate as its major energy and carbon source. Results from a case-controlled study suggested that lack of O. formigenes colonization is a risk factor for recurrent c...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1272-y
更新日期:2016-12-01 00:00:00
abstract::The mutagenic activity of ethyl acetate extracts of culture medium from Cunninghamella elegans incubated 72 h with various polycyclic aromatic hydrocarbons (PAHs) was evaluated in the Salmonella typhimurium reversion assay. All of the PAH extracts were assayed in tester strains TA98 and TA100 both with and without met...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00411031
更新日期:1985-11-01 00:00:00
abstract::The fast-growing Rhizobium sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of Sinorhizobium fredii, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isol...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-003-0559-y
更新日期:2003-07-01 00:00:00
abstract::Plasmid-borne hup-lacZ transcriptional fusion constructs were introduced into three separate mutant strains of Bradyrhizobium japonicum which express hydrogenase constitutively (Hupc strains SR470, SR473 and JH101) in both autotrophic and heterotrophic environments. The lacZ structural gene linked directly to the regu...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00258144
更新日期:1993-01-01 00:00:00
abstract::A 55 kilobase (kb) plasmid (pOZS550) in the non-clumping Lactococcus lactis subsp. lactis strain OZS1 carrying genes for lactose metabolism was characterised. A mobilizable cointegrate plasmid which is formed between pOZS550 and pOZS448 carries the necessary information for conjugation and transfer. Cointegrate format...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00248837
更新日期:1990-01-01 00:00:00
abstract::The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid. ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00423075
更新日期:1979-03-12 00:00:00
abstract::A novel actinomycete, strain PA1-10T, isolated from the leaf of Phyllanthus amarus collected from Bangkok, Thailand, was characterized taxonomically using a polyphasic approach. This strain contained the characteristics consistent with those of members of the genus Nonomuraea. It formed short rugose spore chain on aer...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01717-w
更新日期:2020-01-01 00:00:00
abstract::The mechanism of lysis of Eubacterium alactolyticum cell walls by Streptomyces albus G enzyme was studied. The analysis of the peptide terminal groups and peptide subunits isolated from the cell wall digest, released during solubilization of the cell walls, revealed that lytic action of S. albus G enzyme was mainly du...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00406563
更新日期:1989-01-01 00:00:00
abstract::The antimicrobial properties of copper ions have been known for a long time. However, the exact mechanism of action of the transition metal on microorganisms has long been unclear. X-ray absorption near-edge structure (XANES) spectroscopy at the Cu K edge allows the determination of copper speciation in Staphylococcus...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-017-1454-2
更新日期:2018-04-01 00:00:00
abstract::Bacteroides succinogenes produces acetate and succinate as major products of carbohydrate fermentation. An investigation of the enzymes involved indicated that pyruvate is oxidized by a flavin-dependent pyruvate cleavage enzyme to acetyl-CoA and CO2. Active CO2 exchange is associated with the pyruvate oxidation system...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/BF00402302
更新日期:1978-05-30 00:00:00
abstract::An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex pr...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s002030050745
更新日期:1999-08-01 00:00:00
abstract::This study compared rhizobacterial communities in pepper greenhouses under a paddy-upland (rice-pepper) rotational system (PURS) and a monoculture repeated cropping system (RCS) and examined adverse effects of high salinity on soil properties. The following soil properties were analyzed: electrical conductivity (EC), ...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1304-7
更新日期:2017-03-01 00:00:00
abstract::Rather recently it has become clear that prokaryotes (Archaea and Bacteria) are able to glycosylate proteins. A literature survey revealed the different types of glycoproteins. They include mainly surface layer (S-layer) proteins, flagellins, and polysaccharide-degrading enzymes. Only in a few cases is structural info...
journal_title:Archives of microbiology
pub_type: 杂志文章,评审
doi:10.1007/s002030050484
更新日期:1997-09-01 00:00:00
abstract::Recently, it was proposed that some antibiotics stimulate the production of reactive oxygen species (ROS), which contribute to cell death. Later, other research groups have provided arguments against ROS-mediated killing of bacteria by antibiotics. At present, there remain a number of unanswered questions in understan...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-016-1247-z
更新日期:2016-11-01 00:00:00
abstract::Cryptococcosis is a common opportunistic infection in patients with advanced HIV infection and may also affect immunocompetent patients. The available antifungal agents are few and other options are needed for the cryptococcosis treatment. In this work, we first analyzed the virulence of twelve C. neoformans and C. ga...
journal_title:Archives of microbiology
pub_type: 杂志文章
doi:10.1007/s00203-019-01789-8
更新日期:2020-05-01 00:00:00